Human Anti-FAP Recombinant Antibody (clone ESC14) (CAT#: HPAB-0175-CN)
Antibody ESC14 is a fully human, mouse/human cross-reactive, high affinity anti-FAP antibody that induces relevant regulatory activities on target cells pointing out to their therapeutic impact.
Specific Inquiry
SDS-PAGE
Figure 1 Human Anti-FAP Recombinant Antibody (HPAB-0175-CN) in SDS-PAGE
SDS-PAGE analysis of HPAB-0175-CN in β-mercaptoethanol-reduced (Lane 1) and non-reduced (Lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively. And non-reduced protein migrates as about 135-245 kDa.
SEC-HPLC
Figure 2 Human Anti-FAP Recombinant Antibody (HPAB-0175-CN) in SEC-HPLC
The purity of HPAB-0175-CN was greater than 95% as determined by SEC-HPLC.
Column: 3 µm, 7.8 x 300 nm
Mobile phase: 150 mM Sodium Phosphate Buffer, pH 7.0
Detection: UV 280 nm
Injection: 10 µl
DB
Figure 3 Human Anti-FAP Recombinant Antibody (HPAB-0175-CN) in DB
Dot blot analysis of HPAB-0175-CN was performed by loading human FAP protein active dimer (His Tag).
HPAB-0175-CN incubation concentration: 2 ng/μL.
The secondary antibody: HRP-Anti-Human IgG (H+L)
WB
Figure 4 Human Anti-FAP Recombinant Antibody (HPAB-0175-CN) in WB
Western blot analysis of HPAB-0175-CN was performed by loading human FAP protein active dimer (His Tag).
HPAB-0175-CN incubation concentration: 2 ng/μL.
The secondary antibody: HRP-Anti-Human IgG (H+L)
Lane 1: Reducing antigen (0.1 μg)
Lane 2: Reducing antigen (0.3 μg)
Lane 3: Reducing antigen (0.6 μg)
ELISA
Figure 5 Human Anti-FAP Recombinant Antibody (HPAB-0175-CN) in ELISA
ELISA analysis of HPAB-0175-CN was performed by coating with human FAP protein active dimer (His Tag).
The secondary antibody: HRP-Anti-Human IgG (H+L)
Specifications
- Immunogen
- Recombinant human FAP
- Host Species
- Human
- Type
- Human IgG1
- Specificity
- Human FAP
- Species Reactivity
- Human, Mouse
- Clone
- ESC14
- Applications
- ELISA, WB, FC, IHC, IP
- Related Disease
- Cancer
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- FAP; fibroblast activation protein, alpha; FAPA; SIMP; DPPIV; prolyl endopeptidase FAP; seprase; FAPalpha; dipeptidyl peptidase FAP; surface-expressed protease; post-proline cleaving enzyme; gelatine degradation protease FAP; integral membrane serine protease; serine integral membrane protease; 170 kDa melanoma membrane-bound gelatinase;
- Gene ID
- 2191
- UniProt ID
- B4DLR2
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Antibody Magnetic Beads
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2277z | Mouse Anti-FAP Recombinant Antibody (clone 36B12) | FC, IF, IP | Mouse IgG1 |
| HPAB-0172-CN | Human Anti-FAP Recombinant Antibody (HPAB-0172-CN) | ELISA, IHC | Chimeric (mouse/human) IgG1, κ |
| HPAB-0176-CN | Human Anti-FAP Recombinant Antibody (clone cF19) | ELISA, FC, IHC, IP, IF | Chimeric (mouse/human) IgG1 |
| HPAB-2061-FY | Human Anti-FAP Recombinant Antibody (HPAB-2061-FY) | SPR, IHC | Human IgG1 |
| HPAB-2062-FY | Human Anti-FAP Recombinant Antibody (HPAB-2062-FY) | FC | Human IgG1 |
Customer Reviews and Q&As
Customer Review
Q&As
-
Consistent Quality
Kept the same level of quality over various batches, which is essential for further research. -
Worked in Difficult Samples
Exhibited good handling of challenging samples, yielding dependable results in the absence of other antibodies. -
Versatile Use
This antibody was used in several applications, and it worked incredibly well in each and every one of them.
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# HPAB-0175-CN, RRID: AB_3111365)
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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