Human Anti-FAP Recombinant Antibody (clone ESC14) (CAT#: HPAB-0175-CN)

Antibody ESC14 is a fully human, mouse/human cross-reactive, high affinity anti-FAP antibody that induces relevant regulatory activities on target cells pointing out to their therapeutic impact.

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SDS-PAGE

Figure 1 Human Anti-FAP Recombinant Antibody (HPAB-0175-CN) in SDS-PAGE

Figure 1 Human Anti-FAP Recombinant Antibody (HPAB-0175-CN) in SDS-PAGE

SDS-PAGE analysis of HPAB-0175-CN in β-mercaptoethanol-reduced (Lane 1) and non-reduced (Lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively. And non-reduced protein migrates as about 135-245 kDa.

SEC-HPLC

Figure 2 Human Anti-FAP Recombinant Antibody (HPAB-0175-CN) in SEC-HPLC

Figure 2 Human Anti-FAP Recombinant Antibody (HPAB-0175-CN) in SEC-HPLC

The purity of HPAB-0175-CN was greater than 95% as determined by SEC-HPLC.

Column: 3 µm, 7.8 x 300 nm
Mobile phase: 150 mM Sodium Phosphate Buffer, pH 7.0
Detection: UV 280 nm
Injection: 10 µl

DB

Figure 3 Human Anti-FAP Recombinant Antibody (HPAB-0175-CN) in DB

Figure 3 Human Anti-FAP Recombinant Antibody (HPAB-0175-CN) in DB

Dot blot analysis of HPAB-0175-CN was performed by loading human FAP protein active dimer (His Tag).

HPAB-0175-CN incubation concentration: 2 ng/μL.
The secondary antibody: HRP-Anti-Human IgG (H+L)

WB

Figure 4 Human Anti-FAP Recombinant Antibody (HPAB-0175-CN) in WB

Figure 4 Human Anti-FAP Recombinant Antibody (HPAB-0175-CN) in WB

Western blot analysis of HPAB-0175-CN was performed by loading human FAP protein active dimer (His Tag).

HPAB-0175-CN incubation concentration: 2 ng/μL.
The secondary antibody: HRP-Anti-Human IgG (H+L)
Lane 1: Reducing antigen (0.1 μg)
Lane 2: Reducing antigen (0.3 μg)
Lane 3: Reducing antigen (0.6 μg)

ELISA

Figure 5 Human Anti-FAP Recombinant Antibody (HPAB-0175-CN) in ELISA

Figure 5 Human Anti-FAP Recombinant Antibody (HPAB-0175-CN) in ELISA

ELISA analysis of HPAB-0175-CN was performed by coating with human FAP protein active dimer (His Tag).
The secondary antibody: HRP-Anti-Human IgG (H+L)


Specifications

  • Immunogen
  • Recombinant human FAP
  • Host Species
  • Human
  • Type
  • Human IgG1
  • Specificity
  • Human FAP
  • Species Reactivity
  • Human, Mouse
  • Clone
  • ESC14
  • Applications
  • ELISA, WB, FC, IHC, IP
  • Related Disease
  • Cancer

Product Property

  • Purity
  • >95% as determined by SDS-PAGE and HPLC analysis
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Buffer
  • PBS
  • Preservative
  • No preservatives
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

  • Alternative Names
  • FAP; fibroblast activation protein, alpha; FAPA; SIMP; DPPIV; prolyl endopeptidase FAP; seprase; FAPalpha; dipeptidyl peptidase FAP; surface-expressed protease; post-proline cleaving enzyme; gelatine degradation protease FAP; integral membrane serine protease; serine integral membrane protease; 170 kDa melanoma membrane-bound gelatinase;

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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