What is the immunogen?

The antibody reacts with a recombinant fragment corresponding to amino acids 525-625 of Human Fibroblast activation protein, alpha (FAP).

What species does it react with?

This recombinant mouse monoclonal antibody specifically reacts with Human FAP. It is validated for use in Western Blot applications.

Recombinant Mouse Anti-Human FAP Antibody

CAT#: MOB-1515MZ

This antibody is a recombinant mouse monoclonal antibody which specifically reacts with human FAP.

Gene Expression

Normal Tissue Figure 1 Colon

Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/59739/134103_A_9_3.jpg

Normal Tissue Figure 2 Liver

Cholangiocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/59739/134103_A_8_4.jpg

Normal Tissue Figure 3 Kidney

Cells in tubules
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/59739/134103_A_7_5.jpg

Normal Tissue Figure 4 Testis

Cells in seminiferous ducts
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/59739/134103_A_4_6.jpg

Normal Tissue Figure 5 Smooth muscle

Smooth muscle cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/59739/134103_B_6_7.jpg

RNA Expression Figure 6 RNA cell line category: Cell line enhanced (ASC diff, ASC TERT1, BJ hTERT+, hTERT-RPE1, U-138 MG)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000078098-FAP

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Specifications

Immunogen
Recombinant fragment: PPQFDRSKKY PLLIQVYGGP CSQSVRSVFA VNWISYLASK EGMVIALVDG RGTAFQGDKL LYAVYRKLGV YEVEDQITAV RKFIEMGFID EKRIAIWGWS , corresponding to amino acids 525-625 of Human Fibroblast activation protein, alpha

Host Species
Mouse

Type
Mouse antibody

Specificity
Human FAP

Species Reactivity
Human

Applications
WB

Target

Alternative Names
FAP; Human FAP

Gene ID
2191

UniProt ID
Q12884

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Breast cancer biomarkers at key points during disease progression

Specific For FAP Alpha

We used this recombinant antibody to detect Fibroblast Activation Protein in tumor stromal cells. It worked well in Western Blot, showing a specific band. The antibody quality from CHO cells is high.

Targets Amino Acids 525-625

This antibody was raised against a specific fragment of human FAP. We found it to be very specific for the target protein in our lysates. It is a reliable monoclonal antibody.

Q&As

What is the immunogen?

A: The antibody reacts with a recombinant fragment corresponding to amino acids 525-625 of Human Fibroblast activation protein, alpha (FAP).
What species does it react with?

A: This recombinant mouse monoclonal antibody specifically reacts with Human FAP. It is validated for use in Western Blot applications.

View the frequently asked questions answered by Creative Biolabs Support.

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Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - MOB-1515MZ.pdf

MSDS

MSDS - MOB-1515MZ.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

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