Mouse Anti-IL2 Recombinant Antibody (TAB-559LC) (CAT#: TAB-559LC)
This product is a recombinant mouse antibody that recognizes IL2. The antibody was purified by affinity chromatography.
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
Figure 1 Anti-human IL2 Recombinant antibody (TAB-559LC) in ELISA
ELISA analysis of TAB-559LC was performed by coating with human IL2 protein (His tag). Then blocked with BSA and incubated with TAB-559LC. The HRP-conjugated goat anti-IgG was used as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Figure 2 Anti-human IL2 Recombinant antibody (TAB-559LC) in WB
Western blot analysis of TAB-559LC was performed by loading human IL2 protein onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with TAB-559LC and a HRP goat anti-IgG (H+L) was used as the secondary antibody (1: 3000). Chemiluminescent detection was performed.
Figure 3 Anti-human IL2 Recombinant antibody (TAB-559LC) in DB
Dot Blot analysis of TAB-559LC was performed by coating with human IL2 protein (His tag).
TAB-559LC incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-IgG (H+L) secondary antibody (1: 3000)
NC: Negative control, PC: Positive control
Specifications
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human IL-2
- Species Reactivity
- Human
- Applications
- ELISA, FC, FuncS
- Related Disease
- Cancer, Viral infection
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
Related Resources
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "Il2"
Select a product category from the dropdown menu below to view related products.
CAT | Product Name | Application | Type |
---|---|---|---|
NABG-093 | Recombinant Anti-Mouse Il2 VHH Single Domain Antibody | ELISA, IHC, FC, FuncS | Llama VHH |
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For Research Use Only. Not For Clinical Use.
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