Mouse Anti-IL2 Recombinant Antibody (TAB-559LC) (CAT#: TAB-559LC)

This product is a recombinant mouse antibody that recognizes IL2. The antibody was purified by affinity chromatography.

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ELISA

Figure 1 Anti-human IL2 Recombinant antibody (TAB-559LC) in ELISA

Figure 1 Anti-human IL2 Recombinant antibody (TAB-559LC) in ELISA

ELISA analysis of TAB-559LC was performed by coating with human IL2 protein (His tag). Then blocked with BSA and incubated with TAB-559LC. The HRP-conjugated goat anti-IgG was used as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.

WB

Figure 2 Anti-human IL2 Recombinant antibody (TAB-559LC) in WB

Figure 2 Anti-human IL2 Recombinant antibody (TAB-559LC) in WB

Western blot analysis of TAB-559LC was performed by loading human IL2 protein onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with TAB-559LC and a HRP goat anti-IgG (H+L) was used as the secondary antibody (1: 3000). Chemiluminescent detection was performed.

DB

Figure 3 Anti-human IL2 Recombinant antibody (TAB-559LC) in DB

Figure 3 Anti-human IL2 Recombinant antibody (TAB-559LC) in DB

Dot Blot analysis of TAB-559LC was performed by coating with human IL2 protein (His tag).
TAB-559LC incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-IgG (H+L) secondary antibody (1: 3000)
NC: Negative control, PC: Positive control


Specifications

  • Host Species
  • Mouse
  • Type
  • Mouse IgG
  • Specificity
  • Human IL-2
  • Species Reactivity
  • Human
  • Applications
  • ELISA, FC, FuncS
  • Related Disease
  • Cancer, Viral infection

Product Property

  • Purity
  • >95% as determined by SDS-PAGE
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Buffer
  • PBS
  • Preservative
  • No preservatives
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

  • Alternative Names
  • IL2; Interleukin 2; IL-2; TCGF; lymphokine

Related Resources

  • Related Diseases

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

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CAT Product Name Application Type
NABG-093 Recombinant Anti-Mouse Il2 VHH Single Domain Antibody ELISA, IHC, FC, FuncS Llama VHH

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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