Mouse Anti-Il2 Recombinant Antibody (PABL-609) (CAT#: PABL-609)
Recombinant Mouse Antibody is capable of binding to IL-2, expressed in Chinese Hamster Ovary cells (CHO).
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Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
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ELISA
Figure 1 Mouse Anti-Il2 Recombinant Antibody (PABL-609) in ELISA
ELISA analysis of PABL-609 was performed by coating with mouse il2 protein (His tag). Then blocked with BSA and incubated with PABL-609. The HRP-conjugated goat anti-IgG was used as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
WB
Figure 2 Mouse Anti-Il2 Recombinant Antibody (PABL-609) in WB
Western blot analysis of PABL-609 was performed by loading mouse il2 protein onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with PABL-609 and a HRP goat anti-IgG (H+L) was used as the secondary antibody (1: 3000). Chemiluminescent detection was performed.
DB
Figure 3 Mouse Anti-Il2 Recombinant Antibody (PABL-609) in DB
Dot Blot analysis of PABL-609 was performed by coating with mouse il2 protein (His tag).
PABL-609 incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-IgG (H+L) secondary antibody (1: 3000)
NC: Negative control, PC: Positive control
Specifications
- Immunogen
- Mouse interleukin 2
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Mouse Il2
- Species Reactivity
- Mouse
- Applications
- FC, FuncS, IHC, ELISA
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been reported for use in Flow Cytometry, Function Assay, Immunohistochemistry and Enzyme-Linked Immunosorbent Assay.
Target
- Alternative Names
- IL2; interleukin 2; Il-2; interleukin-2; TCGF; T-cell growth factor
- Gene ID
- 16183
- UniProt ID
- P04351
Related Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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ScFv-Fc Chimera Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NABG-093 | Recombinant Anti-Mouse Il2 VHH Single Domain Antibody | ELISA, IHC, FC, FuncS | Llama VHH |
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To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# PABL-609, RRID: AB_3111664)
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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