Mouse Anti-Il2 Recombinant Antibody (clone S4B6) (CAT#: PABL-610)
Recombinant Mouse Antibody (S4B6) is capable of binding to IL-2, expressed in Chinese Hamster Ovary cells (CHO). This antibody sterically blocked the IL-2:IL-2Rα interaction, while also conformationally stabilizing the IL-2:IL-2Rβ interaction, thus stimulating all IL-2-responsive immune cells, particularly IL-2Rβ(hi) effector cells.
Specific Inquiry
ELISA
Figure 1 Anti-IL-2 antibodies and IL-2 receptor compete for cytokine binding.
Yeast surface mIL-2 competition studies between anti-mIL-2 antibodies (Abs) and saturating concentrations of mIL-2Rα are shown (error bars, SD). Data are representative of three independent experiments.
Spangler, J. B., Tomala, J., Luca, V. C., Jude, K. M., Dong, S., Ring, A. M., ... & Garcia, K. C. (2015). Antibodies to interleukin-2 elicit selective T cell subset potentiation through distinct conformational mechanisms. Immunity, 42(5), 815-825.
FC
Figure 2 IL-2Rα expression histograms for Treg cells isolated from treated mouse spleens.
Representative plots from one mouse per cohort are presented.
Spangler, J. B., Tomala, J., Luca, V. C., Jude, K. M., Dong, S., Ring, A. M., ... & Garcia, K. C. (2015). Antibodies to interleukin-2 elicit selective T cell subset potentiation through distinct conformational mechanisms. Immunity, 42(5), 815-825.
FuncS
Figure 3 Anti-IL-2 antibodies bias cytokine signaling and functional outcomes.
C57BL/6 mouse spleen effector cell: Treg cell ratios after immunocomplex treatment. Data are representative of two independent experiments.
Spangler, J. B., Tomala, J., Luca, V. C., Jude, K. M., Dong, S., Ring, A. M., ... & Garcia, K. C. (2015). Antibodies to interleukin-2 elicit selective T cell subset potentiation through distinct conformational mechanisms. Immunity, 42(5), 815-825.
Specifications
- Immunogen
- Mouse interleukin 2
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Mouse Il2
- Species Reactivity
- Mouse
- Clone
- S4B6
- Applications
- ELISA, FC, FuncS
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been reported for use in Flow Cytometry, Function Assay and Enzyme-Linked Immunosorbent Assay.
Target
- Alternative Names
- IL2; interleukin 2; Il-2; interleukin-2; TCGF; T-cell growth factor
- Gene ID
- 16183
- UniProt ID
- P04351
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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Flow Cytometry (FC) related Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NABG-093 | Recombinant Anti-Mouse Il2 VHH Single Domain Antibody | ELISA, IHC, FC, FuncS | Llama VHH |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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