Mouse Anti-Il2 Recombinant Antibody (clone S4B6) (CAT#: PABL-610)

Recombinant Mouse Antibody (S4B6) is capable of binding to IL-2, expressed in Chinese Hamster Ovary cells (CHO). This antibody sterically blocked the IL-2:IL-2Rα interaction, while also conformationally stabilizing the IL-2:IL-2Rβ interaction, thus stimulating all IL-2-responsive immune cells, particularly IL-2Rβ(hi) effector cells.

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ELISA

Figure 1 Anti-IL-2 antibodies and IL-2 receptor compete for cytokine binding.

Yeast surface mIL-2 competition studies between anti-mIL-2 antibodies (Abs) and saturating concentrations of mIL-2Rα are shown (error bars, SD). Data are representative of three independent experiments.

Spangler, J. B., Tomala, J., Luca, V. C., Jude, K. M., Dong, S., Ring, A. M., ... & Garcia, K. C. (2015). Antibodies to interleukin-2 elicit selective T cell subset potentiation through distinct conformational mechanisms. Immunity, 42(5), 815-825.

FC

Figure 2 IL-2Rα expression histograms for Treg cells isolated from treated mouse spleens.

Representative plots from one mouse per cohort are presented.

Spangler, J. B., Tomala, J., Luca, V. C., Jude, K. M., Dong, S., Ring, A. M., ... & Garcia, K. C. (2015). Antibodies to interleukin-2 elicit selective T cell subset potentiation through distinct conformational mechanisms. Immunity, 42(5), 815-825.

FuncS

Figure 3 Anti-IL-2 antibodies bias cytokine signaling and functional outcomes.

C57BL/6 mouse spleen effector cell: Treg cell ratios after immunocomplex treatment. Data are representative of two independent experiments.

Spangler, J. B., Tomala, J., Luca, V. C., Jude, K. M., Dong, S., Ring, A. M., ... & Garcia, K. C. (2015). Antibodies to interleukin-2 elicit selective T cell subset potentiation through distinct conformational mechanisms. Immunity, 42(5), 815-825.


Specifications

  • Immunogen
  • Mouse interleukin 2
  • Host Species
  • Mouse
  • Type
  • Mouse IgG
  • Specificity
  • Mouse Il2
  • Species Reactivity
  • Mouse
  • Clone
  • S4B6
  • Applications
  • ELISA, FC, FuncS

Applications

  • Application Notes
  • This antibody has been reported for use in Flow Cytometry, Function Assay and Enzyme-Linked Immunosorbent Assay.

Target

  • Alternative Names
  • IL2; interleukin 2; Il-2; interleukin-2; TCGF; T-cell growth factor

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

See other products for "IL2"

See other products for "Clone S4B6"


For Research Use Only. Not For Clinical Use.

* Abbreviations
3D IHC3D Immunohistochemistry
ActivActivation
AgonistAgonist
ApopApoptosis
BABioassay
BIBioimaging
BlockBlocking
Cell ScreeningCell Screening
SeparationCell Separation
ChIPChromatin Immunoprecipitation
CMCDComplement Mediated Cell Depletion
CostimCostimulation
CytCytotoxicity
DepletionDepletion
DBDot Blot
EMElectron Microscopy
ELISAEnzyme-linked Immunosorbent Assay
ELISPOTEnzyme-linked Immunosorbent Spot
FCFlow Cytometry
FuncSFunctional Assay
GSGel Super Shift Assay
HAHemagglutination
IAImmunoassay
IBImmunoblotting
ICCImmunocytochemistry
IDImmunodiffusion
IFImmunofluorescence
IHCImmunohistochemistry
IHC-FrImmunohistochemistry-Frozen
IHC-PImmunohistochemistry-Paraffin
REImmunohistology - Resin Sections
IPImmunoprecipitation
IRMAImmunoradiometric Assay
SHIn situ hybridization
InhibInhibition
ICFCIntracellular Staining for Flow Cytometry
KO/KD-WBKnockout/Knockdown target confirmation by Western Blot
Live cell imagingLive cell imaging
CyTOF®Mass Cytometry
MeDIPMethylated DNA Immunoprecipitation
MultiplexMultiplex bead-based assay
NeutNeutralization
PPProtein Purification
PGProteogenomics
RIRadial Immunodiffusion
RIARadioimmunoassay
StimStimulation
SPRSurface Plasmon Resonance
TCTissue Culture
TBTurbidimetry
WBWestern Blot

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