Human Anti-IL4R Recombinant Antibody (TAB-433CL) (CAT#: TAB-433CL)

This product is a recombinant human antibody targeting human Interleukin-4 receptor subunit alpha (IL4RA), which is a receptor for both interleukin 4 and interleukin 13. The antibody was purified by affinity chromatography. It specifically recognizes human IL4R. It also has potent affinity against IL4RA.


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ELISA

Figure 1 Human Anti-IL4R Recombinant Antibody in ELISA

Figure 1 Human Anti-IL4R Recombinant Antibody in ELISA

ELISA analysis of TAB-433CL was performed by coating with human IL4R protein (His tag). Then blocked with BSA and incubated with TAB-433CL. The HRP-conjugated goat anti-IgG was used as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.

WB

Figure 2 Human Anti-IL4R Recombinant Antibody in WB

Figure 2 Human Anti-IL4R Recombinant Antibody in WB

Western blot analysis of TAB-433CL was performed by loading human IL4R protein onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with TAB-433CL and a HRP goat anti-IgG (H+L) was used as the secondary antibody (1: 3000). Chemiluminescent detection was performed.

DB

Figure 3 Human Anti-IL4R Recombinant Antibody in DB

Figure 3 Human Anti-IL4R Recombinant Antibody in DB

Dot Blot analysis of TAB-433CL was performed by coating with human IL4R protein (His tag).
TAB-433CL incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-IgG (H+L) secondary antibody (1: 3000)
NC: Negative control, PC: Positive control


Specifications

  • Host Species
  • Human
  • Derivation
  • Human
  • Type
  • Human IgG
  • Species Reactivity
  • Human
  • Applications
  • FuncS
  • Related Disease
  • Asthma

Product Property

  • Purity
  • >95% as determined by SDS-PAGE
  • Buffer
  • PBS
  • Preservative
  • No preservatives
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • This antibody has been tested for use in Functional Assay. It's recommended that the optimal antibody concentration, dilution, incubition time, etc. be carefully titrated in specific assays.

Target

  • Alternative Names
  • IL4R; interleukin 4 receptor; CD124; IL4RA; IL-4RA; interleukin-4 receptor subunit alpha; IL4R nirs variant 1; IL-4 receptor subunit alpha; interleukin-4 receptor alpha chain

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "IL4R"

Immunotoxin

CAT Product Name Application Type
AGTO-L079E IL4-PE immunotoxin Cytotoxicity assay, Functional assay
AGTO-L079D IL4-DT immunotoxin Cytotoxicity assay, Functional assay

ADCC Enhanced Antibody

CAT Product Name Application Type
AFC-416CL Afuco™ Anti-IL4R Recombinant Antibody (AFC-416CL), ADCC Enhanced ELISA, WB Human IgG1

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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