IL4-PE immunotoxin

Name: IL4-PE immunotoxin
SKU: AGTO-L079E
Rating: 5 (1 reviews)

CAT#: AGTO-L079E

This immunotoxin was achieved by conjugating/fusing the IL-4 to Pseudomonas exotoxin. It was expressed in CHO and purified with affinity chromatography. The immunotoxin selectively binds to acute lymphoblastic leukemia (ALL) cells and enters cells by receptor-mediated endocytosis, then PE cleaved fragments the size of Mr 37000 and 27000, the large fragment and finally into the Golgi and elongation factor 2 (EM) combining it glycosylation inactivation, inhibition of protein synthesis, leading to apoptosis. It was designed for treatment of acute lymphoblastic leukemia (ALL).

Gene Expression

Subcellular Location Figure 1 IF staining of human cell line HEL

Immunofluorescent staining of human cell line HEL shows localization to nucleoplasm, plasma membrane & centriolar satellites.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/70380/1812_F10_5_cr59ef13a97c5ff_selected.jpg

Normal Tissue Figure 2 Tonsil

High expression in tonsil

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/50124/ihc_selected.jpg

Normal Tissue Figure 3 IHC staining of human tonsil

Immunohistochemical staining of human tonsil shows distinct cytoplasmic positivity in subsets of cells.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4451/ihc_selected.jpg

Normal Tissue Figure 4 Colon

Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/50124/123264_A_9_3.jpg

Normal Tissue Figure 5 Kidney

Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/50124/123264_A_8_5.jpg

RNA Expression Figure 6 RNA cell line category: Cell line enhanced (Daudi, HBEC3-KT, HDLM-2, U-698)

Cell lines ordered by descending RNA expression order

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000077238-IL4R

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Specifications

Host Species
Human

Species Reactivity
Human

Molecule Class
IL4-PE

Toxin Description
Pseudomonas aeruginosa exotoxin (Pseudomonas Aeruginosa Exotoxin A, PE) is a cell toxin produced by P. aeruginosa. Divided into three domains in space. DomainIII (s 405~613 amino acids) having ADP ribosylation activity, can inhibit protein synthesis, PE on toxicity mechanisms of cell depends on its Domainlll. After PE molecules into mammals, Domain Ia and widely distributed in mammalian cells α 2 macroglobulin receptor binding was endocytic, then PE cleaved fragments the size of Mr 37000 and 27000, the large fragment and finally into the Golgi and elongation factor 2 (EM) combining it glycosylation inactivation, inhibition of protein synthesis, leading to apoptosis.

Applications
This product is suitable for use in cytotoxicity assay, functional assay and immunotherapy.

Related Disease
Acute lymphoblastic leukemia (ALL)

Target

Alternative Names
CD124; IL 4R alpha; IL-4 receptor subunit alpha; IL-4-binding protein; IL-4R subunit alpha; IL-4R-alpha; IL-4RA; IL4-BP; Il4r; IL4R nirs variant 1; IL4RA; IL4RA_HUMAN; Interleukin 4 receptor alpha chain; Interleukin 4 receptor; sIL4Ralpha/prot; Soluble IL-4 receptor subunit alpha; Soluble IL-4R-alpha; Soluble interleukin-4 receptor subunit alpha; Pseudomonas exotoxin; Pseudomonas exotoxin A; Pseudomonas aeruginosa exotoxin; Pseudomonas aeruginosa exotoxin A; PE; PE38; PE40; ETA; PE33; PE35

Gene ID
3566

UniProt ID
P24394

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - AGTO-L079E.pdf

MSDS

MSDS - AGTO-L079E.pdf

COA

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