IL4-DT immunotoxin (CAT#: AGTO-L079D)
This immunotoxin was achieved by conjugating/fusing the IL-4 to Diphtheriatoxin. It was expressed in CHO and purified with affinity chromatography. The immunotoxin selectively binds to acute lymphoblastic leukemia (ALL) cells. Once bind, the toxin enters cells by receptor-mediated endocytosis, then Before the N-terminal 193 amino acids corresponding to the catalytic domain of DT enzyme; -II binding and elongation factor into the eukaryotic cell; the cell can catalyze specific elongation factor EF2 amide His699 of diphtheria ADP2 ribosylation occurs; thereby preventing protein synthesis and causes cell death. It was designed for treatment of acute lymphoblastic leukemia (ALL).
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line HEL
(Immunofluorescent staining of human cell line HEL shows localization to nucleoplasm, plasma membrane & centriolar satellites.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/70380/1812_F10_5_cr59ef13a97c5ff_selected.jpg
(Immunofluorescent staining of human cell line HEL shows localization to nucleoplasm, plasma membrane & centriolar satellites.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/70380/1812_F10_5_cr59ef13a97c5ff_selected.jpg
Figure 2 Tonsil
(High expression in tonsil)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/50124/ihc_selected.jpg
(High expression in tonsil)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/50124/ihc_selected.jpg
Figure 3 IHC staining of human tonsil
(Immunohistochemical staining of human tonsil shows distinct cytoplasmic positivity in subsets of cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4451/ihc_selected.jpg
(Immunohistochemical staining of human tonsil shows distinct cytoplasmic positivity in subsets of cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4451/ihc_selected.jpg
Figure 4 Colon
(Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/50124/123264_A_9_3.jpg
(Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/50124/123264_A_9_3.jpg
Figure 5 Kidney
(Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/50124/123264_A_8_5.jpg
(Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/50124/123264_A_8_5.jpg
Figure 6 RNA cell line category: Cell line enhanced (Daudi, HBEC3-KT, HDLM-2, U-698)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000077238-IL4R
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000077238-IL4R
Specifications
- Host Species
- Human
- Species Reactivity
- Human
- Molecule Class
- IL4-DT
- Toxin Description
- Diphtheria toxin (Diphtheriatoxin, DT) is a cell toxin produced by diphtheria, is a protein toxin most commonly used in the construction of recombinant immunotoxin. Before the N-terminal 193 amino acids corresponding to the catalytic domain of DT enzyme, -II binding and elongation factor into the eukaryotic cell, the cell can catalyze specific elongation factor EF2 amide His699 of diphtheria ADP2 ribosylation occurs, thereby preventing protein synthesis and causes cell death.
- Applications
- This product is suitable for use in cytotoxicity assay, functional assay and immunotherapy.
- Related Disease
- Acute lymphoblastic leukemia (ALL)
Target
- Alternative Names
- CD124; IL 4R alpha; IL-4 receptor subunit alpha; IL-4-binding protein; IL-4R subunit alpha; IL-4R-alpha; IL-4RA; IL4-BP; Il4r; IL4R nirs variant 1; IL4RA; IL4RA_HUMAN; Interleukin 4 receptor alpha chain; Interleukin 4 receptor; sIL4Ralpha/prot; Soluble IL-4 receptor subunit alpha; Soluble IL-4R-alpha; Soluble interleukin-4 receptor subunit alpha; Diphtheria toxin; Diphtheria-toxin; DT; DT388; DT389; DT390; DAB389
- Gene ID
- 3566
- UniProt ID
- P24394
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| AGTO-L079E | IL4-PE immunotoxin | Cytotoxicity assay, Functional assay |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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