Mouse Anti-Il2 Recombinant Antibody (clone JES6-1) (CAT#: PABL-609)

Recombinant Mouse Antibody (JES6-1) is capable of binding to IL-2, expressed in Chinese Hamster Ovary cells (CHO).

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FC

Figure 1 Rapid and widespread proliferation of T reg cells after IL-2-JES6-1 treatment.

Figure 1 Rapid and widespread proliferation of T reg cells after IL-2-JES6-1 treatment.

Expansion of T reg cells in the spleen after injection of IL-2-JES6-1. Flow cytometry plots depict log10 fluorescence.

Webster, K. E., Walters, S., Kohler, R. E., Mrkvan, T., Boyman, O., Surh, C. D., ... & Sprent, J. (2009). In vivo expansion of T reg cells with IL-2–mAb complexes: induction of resistance to EAE and long-term acceptance of islet allografts without immunosuppression. Journal of Experimental Medicine, 206(4), 751-760.

FC

Figure 2 Phenotype of IL-2-JES6-1-expanded T reg cells.

Figure 2 Phenotype of IL-2-JES6-1-expanded T reg cells.

Splenic CD4+CD25+Foxp3+ T reg cells were analyzed on day 3 by flow cytometry for expression of cell-surface molecules, TGF-β and PD-1.

Webster, K. E., Walters, S., Kohler, R. E., Mrkvan, T., Boyman, O., Surh, C. D., ... & Sprent, J. (2009). In vivo expansion of T reg cells with IL-2–mAb complexes: induction of resistance to EAE and long-term acceptance of islet allografts without immunosuppression. Journal of Experimental Medicine, 206(4), 751-760.

FuncS

Figure 3 Phenotype of IL-2-JES6-1-expanded T reg cells.

Figure 3 Phenotype of IL-2-JES6-1-expanded T reg cells.

In vitro suppression of CD4+CD25- T cells (effectors) by FACS-sorted CD4+CD25+ T reg cells (suppressors; in indicated ratios) isolated from mice on day 3 or 7 after treatment. Proliferation of effectors was measured by incorporation of [3H]thymidine.

Webster, K. E., Walters, S., Kohler, R. E., Mrkvan, T., Boyman, O., Surh, C. D., ... & Sprent, J. (2009). In vivo expansion of T reg cells with IL-2–mAb complexes: induction of resistance to EAE and long-term acceptance of islet allografts without immunosuppression. Journal of Experimental Medicine, 206(4), 751-760.

IHC

Figure 4 Long-term allograft survival in IL-2-JES6-1-treated mice.

Figure 4 Long-term allograft survival in IL-2-JES6-1-treated mice.

Representative immunohistochemical staining for Foxp3+ cells in peri-islet mononuclear accumulation (inset; n = 5). Foxp3+ cells are denoted by horseradish peroxidase staining (arrow heads). Data are representative of three independent experiments unless otherwise specified. Bars: 20 µm.

Webster, K. E., Walters, S., Kohler, R. E., Mrkvan, T., Boyman, O., Surh, C. D., ... & Sprent, J. (2009). In vivo expansion of T reg cells with IL-2–mAb complexes: induction of resistance to EAE and long-term acceptance of islet allografts without immunosuppression. Journal of Experimental Medicine, 206(4), 751-760.

IHC

Figure 5 Anti-IL-2 antibodies bias cytokine signaling and functional outcomes.

Figure 5 Anti-IL-2 antibodies bias cytokine signaling and functional outcomes.

Colon histopathology of colitis-induced BALB/c mice pretreated with mIL-2:JES6-1.

Spangler, J. B., Tomala, J., Luca, V. C., Jude, K. M., Dong, S., Ring, A. M., ... & Garcia, K. C. (2015). Antibodies to interleukin-2 elicit selective T cell subset potentiation through distinct conformational mechanisms. Immunity, 42(5), 815-825.

ELISA

Figure 6 Anti-IL-2 antibodies and IL-2 receptor compete for cytokine binding.

Figure 6 Anti-IL-2 antibodies and IL-2 receptor compete for cytokine binding.

Yeast surface mIL-2 competition studies between anti-mIL-2 antibodies (Abs) and saturating concentrations of mIL-2Rα are shown (error bars, SD). Data are representative of three independent experiments.

Spangler, J. B., Tomala, J., Luca, V. C., Jude, K. M., Dong, S., Ring, A. M., ... & Garcia, K. C. (2015). Antibodies to interleukin-2 elicit selective T cell subset potentiation through distinct conformational mechanisms. Immunity, 42(5), 815-825.


Specifications

  • Immunogen
  • Mouse interleukin 2
  • Host Species
  • Mouse
  • Type
  • Mouse IgG
  • Specificity
  • Mouse Il2
  • Species Reactivity
  • Mouse
  • Clone
  • JES6-1
  • Applications
  • FC, FuncS, IHC, ELISA

Product Property

  • Purity
  • >95% as determined by SDS-PAGE and HPLC analysis
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • This antibody has been reported for use in Flow Cytometry, Function Assay, Immunohistochemistry and Enzyme-Linked Immunosorbent Assay.

Target

  • Alternative Names
  • IL2; interleukin 2; Il-2; interleukin-2; TCGF; T-cell growth factor

Related Resources

  • Related Diseases

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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