Mouse Anti-Il2 Recombinant Antibody (clone JES6-1)

Figure 1 Rapid and widespread proliferation of T reg cells after IL-2-JES6-1 treatment.

Expansion of T reg cells in the spleen after injection of IL-2-JES6-1. Flow cytometry plots depict log10 fluorescence.

Webster, K. E., Walters, S., Kohler, R. E., Mrkvan, T., Boyman, O., Surh, C. D., ... & Sprent, J. (2009). In vivo expansion of T reg cells with IL-2–mAb complexes: induction of resistance to EAE and long-term acceptance of islet allografts without immunosuppression. Journal of Experimental Medicine, 206(4), 751-760.

Figure 2 Phenotype of IL-2-JES6-1-expanded T reg cells.

Splenic CD4+CD25+Foxp3+ T reg cells were analyzed on day 3 by flow cytometry for expression of cell-surface molecules, TGF-β and PD-1.

Webster, K. E., Walters, S., Kohler, R. E., Mrkvan, T., Boyman, O., Surh, C. D., ... & Sprent, J. (2009). In vivo expansion of T reg cells with IL-2–mAb complexes: induction of resistance to EAE and long-term acceptance of islet allografts without immunosuppression. Journal of Experimental Medicine, 206(4), 751-760.

Figure 3 Phenotype of IL-2-JES6-1-expanded T reg cells.

In vitro suppression of CD4+CD25- T cells (effectors) by FACS-sorted CD4+CD25+ T reg cells (suppressors; in indicated ratios) isolated from mice on day 3 or 7 after treatment. Proliferation of effectors was measured by incorporation of [3H]thymidine.

Webster, K. E., Walters, S., Kohler, R. E., Mrkvan, T., Boyman, O., Surh, C. D., ... & Sprent, J. (2009). In vivo expansion of T reg cells with IL-2–mAb complexes: induction of resistance to EAE and long-term acceptance of islet allografts without immunosuppression. Journal of Experimental Medicine, 206(4), 751-760.

Figure 4 Long-term allograft survival in IL-2-JES6-1-treated mice.

Representative immunohistochemical staining for Foxp3+ cells in peri-islet mononuclear accumulation (inset; n = 5). Foxp3+ cells are denoted by horseradish peroxidase staining (arrow heads). Data are representative of three independent experiments unless otherwise specified. Bars: 20 µm.

Webster, K. E., Walters, S., Kohler, R. E., Mrkvan, T., Boyman, O., Surh, C. D., ... & Sprent, J. (2009). In vivo expansion of T reg cells with IL-2–mAb complexes: induction of resistance to EAE and long-term acceptance of islet allografts without immunosuppression. Journal of Experimental Medicine, 206(4), 751-760.

Figure 5 Anti-IL-2 antibodies bias cytokine signaling and functional outcomes.

Colon histopathology of colitis-induced BALB/c mice pretreated with mIL-2:JES6-1.

Spangler, J. B., Tomala, J., Luca, V. C., Jude, K. M., Dong, S., Ring, A. M., ... & Garcia, K. C. (2015). Antibodies to interleukin-2 elicit selective T cell subset potentiation through distinct conformational mechanisms. Immunity, 42(5), 815-825.

Figure 6 Anti-IL-2 antibodies and IL-2 receptor compete for cytokine binding.

Yeast surface mIL-2 competition studies between anti-mIL-2 antibodies (Abs) and saturating concentrations of mIL-2Rα are shown (error bars, SD). Data are representative of three independent experiments.

Spangler, J. B., Tomala, J., Luca, V. C., Jude, K. M., Dong, S., Ring, A. M., ... & Garcia, K. C. (2015). Antibodies to interleukin-2 elicit selective T cell subset potentiation through distinct conformational mechanisms. Immunity, 42(5), 815-825.

Figure 1 Mouse Anti-Il2 Recombinant Antibody (PABL-609) in ELISA

ELISA analysis of PABL-609 was performed by coating with mouse il2 protein (His tag). Then blocked with BSA and incubated with PABL-609. The HRP-conjugated goat anti-IgG was used as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.

Figure 2 Mouse Anti-Il2 Recombinant Antibody (PABL-609) in WB

Western blot analysis of PABL-609 was performed by loading mouse il2 protein onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with PABL-609 and a HRP goat anti-IgG (H+L) was used as the secondary antibody (1: 3000). Chemiluminescent detection was performed.

Figure 3 Mouse Anti-Il2 Recombinant Antibody (PABL-609) in DB

Dot Blot analysis of PABL-609 was performed by coating with mouse il2 protein (His tag).
PABL-609 incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-IgG (H+L) secondary antibody (1: 3000)
NC: Negative control, PC: Positive control