Recombinant Mouse Antibody (JES6-1) is capable of binding to IL-2, expressed in Chinese Hamster Ovary cells (CHO).
Figure 1 Rapid and widespread proliferation of T reg cells after IL-2-JES6-1 treatment.
Expansion of T reg cells in the spleen after injection of IL-2-JES6-1. Flow cytometry plots depict log10 fluorescence.
Webster, K. E., Walters, S., Kohler, R. E., Mrkvan, T., Boyman, O., Surh, C. D., ... & Sprent, J. (2009). In vivo expansion of T reg cells with IL-2–mAb complexes: induction of resistance to EAE and long-term acceptance of islet allografts without immunosuppression. Journal of Experimental Medicine, 206(4), 751-760.
Figure 2 Phenotype of IL-2-JES6-1-expanded T reg cells.
Splenic CD4+CD25+Foxp3+ T reg cells were analyzed on day 3 by flow cytometry for expression of cell-surface molecules, TGF-β and PD-1.
Webster, K. E., Walters, S., Kohler, R. E., Mrkvan, T., Boyman, O., Surh, C. D., ... & Sprent, J. (2009). In vivo expansion of T reg cells with IL-2–mAb complexes: induction of resistance to EAE and long-term acceptance of islet allografts without immunosuppression. Journal of Experimental Medicine, 206(4), 751-760.
Figure 3 Phenotype of IL-2-JES6-1-expanded T reg cells.
In vitro suppression of CD4+CD25- T cells (effectors) by FACS-sorted CD4+CD25+ T reg cells (suppressors; in indicated ratios) isolated from mice on day 3 or 7 after treatment. Proliferation of effectors was measured by incorporation of [3H]thymidine.
Webster, K. E., Walters, S., Kohler, R. E., Mrkvan, T., Boyman, O., Surh, C. D., ... & Sprent, J. (2009). In vivo expansion of T reg cells with IL-2–mAb complexes: induction of resistance to EAE and long-term acceptance of islet allografts without immunosuppression. Journal of Experimental Medicine, 206(4), 751-760.
Figure 4 Long-term allograft survival in IL-2-JES6-1-treated mice.
Representative immunohistochemical staining for Foxp3+ cells in peri-islet mononuclear accumulation (inset; n = 5). Foxp3+ cells are denoted by horseradish peroxidase staining (arrow heads). Data are representative of three independent experiments unless otherwise specified. Bars: 20 µm.
Webster, K. E., Walters, S., Kohler, R. E., Mrkvan, T., Boyman, O., Surh, C. D., ... & Sprent, J. (2009). In vivo expansion of T reg cells with IL-2–mAb complexes: induction of resistance to EAE and long-term acceptance of islet allografts without immunosuppression. Journal of Experimental Medicine, 206(4), 751-760.
Figure 5 Anti-IL-2 antibodies bias cytokine signaling and functional outcomes.
Colon histopathology of colitis-induced BALB/c mice pretreated with mIL-2:JES6-1.
Spangler, J. B., Tomala, J., Luca, V. C., Jude, K. M., Dong, S., Ring, A. M., ... & Garcia, K. C. (2015). Antibodies to interleukin-2 elicit selective T cell subset potentiation through distinct conformational mechanisms. Immunity, 42(5), 815-825.
Figure 6 Anti-IL-2 antibodies and IL-2 receptor compete for cytokine binding.
Yeast surface mIL-2 competition studies between anti-mIL-2 antibodies (Abs) and saturating concentrations of mIL-2Rα are shown (error bars, SD). Data are representative of three independent experiments.
Spangler, J. B., Tomala, J., Luca, V. C., Jude, K. M., Dong, S., Ring, A. M., ... & Garcia, K. C. (2015). Antibodies to interleukin-2 elicit selective T cell subset potentiation through distinct conformational mechanisms. Immunity, 42(5), 815-825.
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CAT | Product Name | Application | Type |
---|---|---|---|
NABG-093 | Recombinant Anti-Mouse Il2 VHH Single Domain Antibody | ELISA, IHC, FC, FuncS | Llama VHH |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-412CL | Human Anti-IL2 Recombinant Antibody (TAB-412CL) | FuncS | Human IgG1 |
TAB-556LC | Human Anti-IL2 Recombinant Antibody (TAB-556LC) | ELISA, FC | Humanized antibody |
TAB-556LC-S(P) | Human Anti-IL2 Recombinant Antibody; scFv Fragment (TAB-556LC-S(P)) | ELISA, FC | Humanized scFv |
TAB-556LC-F(E) | Human Anti-IL2 Recombinant Antibody; Fab Fragment (TAB-556LC-F(E)) | ELISA, FC | Humanized Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
PSBL-604 | Mouse Anti-Il2 Recombinant Antibody (clone JES6-1); scFv Fragment | Neut | Mouse scFv |
PSBL-605 | Mouse Anti-Il2 Recombinant Antibody (clone S4B6); scFv Fragment | WB, ELISA, FuncS | Mouse scFv |
HPAB-0112-YC-S(P) | Human Anti-IL2 Recombinant Antibody; scFv Fragment (HPAB-0112-YC-S(P)) | ELISA, FC, FuncS | Human scFv |
FAMAB-0179-YC-S(P) | Human Anti-IL2 Recombinant Antibody (clone C5); scFv Fragment | ELISA, WB | Human scFv |
PSBC-506 | Human Anti-IL2 Recombinant Antibody (clone F5111.2); scFv Fragment | ELISA, FC, FuncS | Human scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-557LC | Human Anti-IL2 Recombinant Antibody (TAB-557LC) | ELISA, FC, Inhib, FuncS | Human IgG1 |
TAB-558LC | Human Anti-IL2 Recombinant Antibody (TAB-558LC) | ELISA, FC, FuncS | Human IgG1 |
TAB-557LC-S(P) | Human Anti-IL2 Recombinant Antibody; scFv Fragment (TAB-557LC-S(P)) | ELISA, FC | Human scFv |
TAB-558LC-S(P) | Human Anti-IL2 Recombinant Antibody; scFv Fragment (TAB-558LC-S(P)) | ELISA, FC | Human scFv |
PABX-123-S (P) | Recombinant Mouse Anti-Interleukin 2 Antibody scFv Fragment | WB, ELISA, Neut, FuncS | scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-559LC | Mouse Anti-IL2 Recombinant Antibody (TAB-559LC) | ELISA, FC, FuncS | Mouse IgG |
TAB-559LC-S(P) | Mouse Anti-IL2 Recombinant Antibody; scFv Fragment (TAB-559LC-S(P)) | ELISA, FC | Mouse scFv |
TAB-559LC-F(E) | Mouse Anti-IL2 Recombinant Antibody; Fab Fragment (TAB-559LC-F(E)) | ELISA, FC | Mouse Fab |
PABX-123 | Recombinant Mouse Anti-Interleukin 2 Antibody | WB, ELISA, Neut, FuncS | IgG |
PABX-123-F (E) | Recombinant Mouse Anti-Interleukin 2 Antibody Fab Fragment | WB, ELISA, Neut, FuncS | Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
Gly-182LC | Recombinant Anti-Human IL2 Antibody (Non-glycosylated) | ELISA | Human antibody |
Gly-183LC | Recombinant Anti-Human IL2 Antibody (Non-glycosylated) | ELISA | Human antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
BRD-0283MZ | Chicken Anti-Interleukin-2 Polyclonal IgY | WB, Indirect ELISA | Chicken antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
NEUT-1306CQ | Rat Anti-IL2 Recombinant Antibody (clone 17H12) | Neut | Rat IgG2a |
NEUT-1307CQ | Mouse Anti-IL2 Recombinant Antibody (clone 297C16F11) | IHC, Neut | Mouse IgG2a, κ |
NEUT-1308CQ | Mouse Anti-IL2 Recombinant Antibody (VMC194) | FuncS, Neut | Mouse IgG2a, κ |
NEUT-1310CQ | Mouse Anti-IL2 Recombinant Antibody (clone CBL121) | Neut, WB, IHC | Mouse IgG1 |
NEUT-1311CQ | Mouse Anti-IL2 Recombinant Antibody (clone CBL211) | DB, IHC, Neut | Mouse IgG2a, κ |
CAT | Product Name | Application | Type |
---|---|---|---|
NEUT-1309CQ | Mouse Anti-IL2 Recombinant Antibody (VMC195) | FC, Block | Mouse IgG1 |
NEUT-1315CQ | Rat Anti-Il2 Recombinant Antibody (clone 1A12), Biotin | Block, ELISA, WB | Rat IgG2a |
CAT | Product Name | Application | Type |
---|---|---|---|
MOR-1801 | Rabbit Anti-IL2 Recombinant Antibody (clone DS1801AB) | ICC, IHC-P, WB | Rabbit IgG |
MOR-4612 | Rabbit Anti-IL2 Recombinant Antibody (clone TH125DS) | WB, IHC-P | Rabbit IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
FAMAB-0179-YC-F(E) | Human Anti-IL2 Recombinant Antibody (clone C5); Fab Fragment | ELISA, WB | Human Fab |
HPAB-M0116-YC-F(E) | Mouse Anti-IL2 Recombinant Antibody (clone MAB602); Fab Fragment | ELISA | Mouse Fab |
HPAB-N0149-YC-F(E) | Human Anti-IL2 Recombinant Antibody; Fab Fragment (HPAB-N0149-YC-F(E)) | ELISA, FC | Human Fab |
HPAB-3056LY-F(E) | Human Anti-IL2 Recombinant Antibody; Fab Fragment (HPAB-3056LY-F(E)) | ELISA | Human Fab |
HPAB-3057LY-F(E) | Human Anti-IL2 Recombinant Antibody; Fab Fragment (HPAB-3057LY-F(E)) | ELISA | Human Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
FAMAB-0195CQ | Human Anti-IL2 Recombinant Antibody (clone LNKB-2) | WB, IP, IF, ELISA | Chimeric (mouse/human) IgG4, κ |
PABC-506 | Human Anti-IL2 Recombinant Antibody (clone F5111.2) | Inhib, ELISA, FC, FuncS | Human IgG1, λ |
HPAB-M0116-YC | Mouse Anti-IL2 Recombinant Antibody (clone MAB602) | ELISA, FC | Mouse IgG |
HPAB-3056LY | Human Anti-IL2 Recombinant Antibody (HPAB-3056LY) | ELISA | Human IgG |
HPAB-3057LY | Human Anti-IL2 Recombinant Antibody (HPAB-3057LY) | ELISA | Human IgG |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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