Mouse Anti-Il2 Recombinant Antibody (clone JES6-1) (CAT#: PABL-609)

Recombinant Mouse Antibody (JES6-1) is capable of binding to IL-2, expressed in Chinese Hamster Ovary cells (CHO).

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FC

Figure 1 Rapid and widespread proliferation of T reg cells after IL-2-JES6-1 treatment.

Expansion of T reg cells in the spleen after injection of IL-2-JES6-1. Flow cytometry plots depict log10 fluorescence.

Webster, K. E., Walters, S., Kohler, R. E., Mrkvan, T., Boyman, O., Surh, C. D., ... & Sprent, J. (2009). In vivo expansion of T reg cells with IL-2–mAb complexes: induction of resistance to EAE and long-term acceptance of islet allografts without immunosuppression. Journal of Experimental Medicine, 206(4), 751-760.

FC

Figure 2 Phenotype of IL-2-JES6-1-expanded T reg cells.

Splenic CD4+CD25+Foxp3+ T reg cells were analyzed on day 3 by flow cytometry for expression of cell-surface molecules, TGF-β and PD-1.

Webster, K. E., Walters, S., Kohler, R. E., Mrkvan, T., Boyman, O., Surh, C. D., ... & Sprent, J. (2009). In vivo expansion of T reg cells with IL-2–mAb complexes: induction of resistance to EAE and long-term acceptance of islet allografts without immunosuppression. Journal of Experimental Medicine, 206(4), 751-760.

FuncS

Figure 3 Phenotype of IL-2-JES6-1-expanded T reg cells.

In vitro suppression of CD4+CD25- T cells (effectors) by FACS-sorted CD4+CD25+ T reg cells (suppressors; in indicated ratios) isolated from mice on day 3 or 7 after treatment. Proliferation of effectors was measured by incorporation of [3H]thymidine.

Webster, K. E., Walters, S., Kohler, R. E., Mrkvan, T., Boyman, O., Surh, C. D., ... & Sprent, J. (2009). In vivo expansion of T reg cells with IL-2–mAb complexes: induction of resistance to EAE and long-term acceptance of islet allografts without immunosuppression. Journal of Experimental Medicine, 206(4), 751-760.

IHC

Figure 4 Long-term allograft survival in IL-2-JES6-1-treated mice.

Representative immunohistochemical staining for Foxp3+ cells in peri-islet mononuclear accumulation (inset; n = 5). Foxp3+ cells are denoted by horseradish peroxidase staining (arrow heads). Data are representative of three independent experiments unless otherwise specified. Bars: 20 µm.

Webster, K. E., Walters, S., Kohler, R. E., Mrkvan, T., Boyman, O., Surh, C. D., ... & Sprent, J. (2009). In vivo expansion of T reg cells with IL-2–mAb complexes: induction of resistance to EAE and long-term acceptance of islet allografts without immunosuppression. Journal of Experimental Medicine, 206(4), 751-760.

IHC

Figure 5 Anti-IL-2 antibodies bias cytokine signaling and functional outcomes.

Colon histopathology of colitis-induced BALB/c mice pretreated with mIL-2:JES6-1.

Spangler, J. B., Tomala, J., Luca, V. C., Jude, K. M., Dong, S., Ring, A. M., ... & Garcia, K. C. (2015). Antibodies to interleukin-2 elicit selective T cell subset potentiation through distinct conformational mechanisms. Immunity, 42(5), 815-825.

ELISA

Figure 6 Anti-IL-2 antibodies and IL-2 receptor compete for cytokine binding.

Yeast surface mIL-2 competition studies between anti-mIL-2 antibodies (Abs) and saturating concentrations of mIL-2Rα are shown (error bars, SD). Data are representative of three independent experiments.

Spangler, J. B., Tomala, J., Luca, V. C., Jude, K. M., Dong, S., Ring, A. M., ... & Garcia, K. C. (2015). Antibodies to interleukin-2 elicit selective T cell subset potentiation through distinct conformational mechanisms. Immunity, 42(5), 815-825.


Specifications

  • Immunogen
  • Mouse interleukin 2
  • Host Species
  • Mouse
  • Type
  • Mouse IgG
  • Specificity
  • Mouse Il2
  • Species Reactivity
  • Mouse
  • Clone
  • JES6-1
  • Applications
  • FC, FuncS, IHC, ELISA

Applications

  • Application Notes
  • This antibody has been reported for use in Flow Cytometry, Function Assay, Immunohistochemistry and Enzyme-Linked Immunosorbent Assay.

Target

  • Alternative Names
  • IL2; interleukin 2; Il-2; interleukin-2; TCGF; T-cell growth factor

Related Resources

  • Related Diseases

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

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Single-domain Antibody

CAT Product Name Application Type
NAB-753-VHH Recombinant Anti-Mouse Il2 VHH Single Domain Antibody IHC, ICC, FC, FA, FUNC Llama VHH
NABG-093 Recombinant Anti-Mouse Il2 VHH Single Domain Antibody ELISA, IHC, FC, FuncS Llama VHH

Humanized Antibody

CAT Product Name Application Type
TAB-412CL Human Anti-IL2 Recombinant Antibody (TAB-412CL) FuncS Human IgG1
TAB-556LC Human Anti-IL2 Recombinant Antibody (TAB-556LC) ELISA, FC Humanized antibody
TAB-556LC-S(P) Human Anti-IL2 Recombinant Antibody; scFv Fragment (TAB-556LC-S(P)) ELISA, FC Humanized scFv
TAB-556LC-F(E) Human Anti-IL2 Recombinant Antibody; Fab Fragment (TAB-556LC-F(E)) ELISA, FC Humanized Fab

Recombinant Antibody

CAT Product Name Application Type
PABL-240 Mouse Anti-IL2 Recombinant Antibody (clone LNKB-2) WB, IHC, FuncS Mouse IgG
HPAB-0112-YC Human Anti-IL2 Recombinant Antibody (HPAB-0112-YC) WB, ELISA, FC, IHC Human IgG1
FAMAB-0179YC Human Anti-IL2 Recombinant Antibody (clone C5) ELISA, WB Human IgG
FAMAB-0195CQ Human Anti-IL2 Recombinant Antibody (clone LNKB-2) WB, IP, IF, ELISA Chimeric (mouse/human) IgG4, κ
PABC-506 Human Anti-IL2 Recombinant Antibody (clone F5111.2) Inhib, ELISA, FC, FuncS Human IgG1, λ

scFv Fragment Antibody

CAT Product Name Application Type
PSBL-240 Recombinant Mouse Anti-IL2 Antibody scFv Fragment (LNKB-2) WB, IHC, FuncS scFv
HPAB-0112-YC-S(P) Human Anti-IL2 Recombinant Antibody; scFv Fragment (HPAB-0112-YC-S(P)) ELISA, FC, FuncS Human scFv
FAMAB-0179-YC-S(P) Human Anti-IL2 Recombinant Antibody (clone C5); scFv Fragment ELISA, WB Human scFv
PSBC-506 Human Anti-IL2 Recombinant Antibody (clone F5111.2); scFv Fragment ELISA, FC, FuncS Human scFv
HPAB-N0149-YC-S(P) Human Anti-IL2 Recombinant Antibody; scFv Fragment (HPAB-N0149-YC-S(P)) ELISA, FC Human scFv

Human Antibody

CAT Product Name Application Type
TAB-557LC Human Anti-IL2 Recombinant Antibody (TAB-557LC) ELISA, FC, Inhib, FuncS Human IgG1
TAB-558LC Human Anti-IL2 Recombinant Antibody (TAB-558LC) ELISA, FC, FuncS Human IgG1
TAB-557LC-S(P) Human Anti-IL2 Recombinant Antibody; scFv Fragment (TAB-557LC-S(P)) ELISA, FC Human scFv
TAB-558LC-S(P) Human Anti-IL2 Recombinant Antibody; scFv Fragment (TAB-558LC-S(P)) ELISA, FC Human scFv
TAB-557LC-F(E) Human Anti-IL2 Recombinant Antibody; Fab Fragment (TAB-557LC-F(E)) ELISA, FC Human Fab

Mouse Antibody

CAT Product Name Application Type
TAB-559LC Mouse Anti-IL2 Recombinant Antibody (TAB-559LC) ELISA, FC, FuncS Mouse IgG
TAB-559LC-S(P) Mouse Anti-IL2 Recombinant Antibody; scFv Fragment (TAB-559LC-S(P)) ELISA, FC Mouse scFv
TAB-559LC-F(E) Mouse Anti-IL2 Recombinant Antibody; Fab Fragment (TAB-559LC-F(E)) ELISA, FC Mouse Fab
PABX-123 Recombinant Mouse Anti-Interleukin 2 Antibody WB, ELISA, Neut, FuncS IgG
PABX-123-F (E) Recombinant Mouse Anti-Interleukin 2 Antibody Fab Fragment WB, ELISA, Neut, FuncS Fab

Deglycosylated Antibody (Non-glycosylated IgGs)

CAT Product Name Application Type
Gly-182LC Recombinant Anti-Human IL2 Antibody (Non-glycosylated) ELISA Human antibody
Gly-183LC Recombinant Anti-Human IL2 Antibody (Non-glycosylated) ELISA Human antibody

Chicken IgY Antibody

CAT Product Name Application Type
BRD-0283MZ Chicken Anti-Interleukin-2 Polyclonal IgY WB, Indirect ELISA Chicken antibody

Neutralizing Antibody

CAT Product Name Application Type
NEUT-1306CQ Rat Anti-IL2 Recombinant Antibody (clone 17H12) Neut Rat IgG2a
NEUT-1307CQ Mouse Anti-IL2 Recombinant Antibody (clone 297C16F11) IHC, Neut Mouse IgG2a, κ
NEUT-1308CQ Mouse Anti-IL2 Recombinant Antibody (clone AB12-3G4) FuncS, Neut Mouse IgG2a, κ
NEUT-1310CQ Mouse Anti-IL2 Recombinant Antibody (clone CBL121) Neut, WB, IHC Mouse IgG1
NEUT-1311CQ Mouse Anti-IL2 Recombinant Antibody (clone CBL211) DB, IHC, Neut Mouse IgG2a, κ

Blocking Antibody

CAT Product Name Application Type
NEUT-1309CQ Mouse Anti-IL2 Recombinant Antibody (clone B-G5) FC, Block Mouse IgG1
NEUT-1315CQ Rat Anti-Il2 Recombinant Antibody (clone 1A12), Biotin Block, ELISA, WB Rat IgG2a

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-1801 Rabbit Anti-IL2 Recombinant Antibody (clone DS1801AB) ICC, IHC-P, WB Rabbit IgG
MOR-4612 Rabbit Anti-IL2 Recombinant Antibody (clone TH125DS) WB, IHC-P Rabbit IgG

Fab Fragment Antibody

CAT Product Name Application Type
HPAB-M0116-YC-F(E) Mouse Anti-IL2 Recombinant Antibody (clone MAB602); Fab Fragment ELISA Mouse Fab
HPAB-3056LY-F(E) Human Anti-IL2 Recombinant Antibody; Fab Fragment (HPAB-3056LY-F(E)) ELISA Human Fab
HPAB-3057LY-F(E) Human Anti-IL2 Recombinant Antibody; Fab Fragment (HPAB-3057LY-F(E)) ELISA Human Fab
HPAB-3058LY-F(E) Human Anti-IL2 Recombinant Antibody; Fab Fragment (HPAB-3058LY-F(E)) ELISA Human Fab
HPAB-3059LY-F(E) Human Anti-IL2 Recombinant Antibody; Fab Fragment (HPAB-3059LY-F(E)) ELISA Human Fab

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

* Abbreviations
3D IHC3D Immunohistochemistry
ActivActivation
AgonistAgonist
ApopApoptosis
BABioassay
BIBioimaging
BlockBlocking
Cell ScreeningCell Screening
SeparationCell Separation
ChIPChromatin Immunoprecipitation
CMCDComplement Mediated Cell Depletion
CostimCostimulation
CytCytotoxicity
DepletionDepletion
DBDot Blot
EMElectron Microscopy
ELISAEnzyme-linked Immunosorbent Assay
ELISPOTEnzyme-linked Immunosorbent Spot
FCFlow Cytometry
FuncSFunctional Assay
GSGel Super Shift Assay
HAHemagglutination
IAImmunoassay
IBImmunoblotting
ICCImmunocytochemistry
IDImmunodiffusion
IFImmunofluorescence
IHCImmunohistochemistry
IHC-FrImmunohistochemistry-Frozen
IHC-PImmunohistochemistry-Paraffin
REImmunohistology - Resin Sections
IPImmunoprecipitation
IRMAImmunoradiometric Assay
SHIn situ hybridization
InhibInhibition
ICFCIntracellular Staining for Flow Cytometry
KO/KD-WBKnockout/Knockdown target confirmation by Western Blot
Live cell imagingLive cell imaging
CyTOF®Mass Cytometry
MeDIPMethylated DNA Immunoprecipitation
MultiplexMultiplex bead-based assay
NeutNeutralization
PPProtein Purification
PGProteogenomics
RIRadial Immunodiffusion
RIARadioimmunoassay
StimStimulation
SPRSurface Plasmon Resonance
TCTissue Culture
TBTurbidimetry
WBWestern Blot

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