Mouse Anti-Il2 Recombinant Antibody (clone JES6-1) (CAT#: PABL-609)

Recombinant Mouse Antibody (JES6-1) is capable of binding to IL-2, expressed in Chinese Hamster Ovary cells (CHO).

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FC

Figure 1 Rapid and widespread proliferation of T reg cells after IL-2-JES6-1 treatment.

Expansion of T reg cells in the spleen after injection of IL-2-JES6-1. Flow cytometry plots depict log10 fluorescence.

Webster, K. E., Walters, S., Kohler, R. E., Mrkvan, T., Boyman, O., Surh, C. D., ... & Sprent, J. (2009). In vivo expansion of T reg cells with IL-2–mAb complexes: induction of resistance to EAE and long-term acceptance of islet allografts without immunosuppression. Journal of Experimental Medicine, 206(4), 751-760.

FC

Figure 2 Phenotype of IL-2-JES6-1-expanded T reg cells.

Splenic CD4+CD25+Foxp3+ T reg cells were analyzed on day 3 by flow cytometry for expression of cell-surface molecules, TGF-β and PD-1.

Webster, K. E., Walters, S., Kohler, R. E., Mrkvan, T., Boyman, O., Surh, C. D., ... & Sprent, J. (2009). In vivo expansion of T reg cells with IL-2–mAb complexes: induction of resistance to EAE and long-term acceptance of islet allografts without immunosuppression. Journal of Experimental Medicine, 206(4), 751-760.

FuncS

Figure 3 Phenotype of IL-2-JES6-1-expanded T reg cells.

In vitro suppression of CD4+CD25- T cells (effectors) by FACS-sorted CD4+CD25+ T reg cells (suppressors; in indicated ratios) isolated from mice on day 3 or 7 after treatment. Proliferation of effectors was measured by incorporation of [3H]thymidine.

Webster, K. E., Walters, S., Kohler, R. E., Mrkvan, T., Boyman, O., Surh, C. D., ... & Sprent, J. (2009). In vivo expansion of T reg cells with IL-2–mAb complexes: induction of resistance to EAE and long-term acceptance of islet allografts without immunosuppression. Journal of Experimental Medicine, 206(4), 751-760.

IHC

Figure 4 Long-term allograft survival in IL-2-JES6-1-treated mice.

Representative immunohistochemical staining for Foxp3+ cells in peri-islet mononuclear accumulation (inset; n = 5). Foxp3+ cells are denoted by horseradish peroxidase staining (arrow heads). Data are representative of three independent experiments unless otherwise specified. Bars: 20 µm.

Webster, K. E., Walters, S., Kohler, R. E., Mrkvan, T., Boyman, O., Surh, C. D., ... & Sprent, J. (2009). In vivo expansion of T reg cells with IL-2–mAb complexes: induction of resistance to EAE and long-term acceptance of islet allografts without immunosuppression. Journal of Experimental Medicine, 206(4), 751-760.

IHC

Figure 5 Anti-IL-2 antibodies bias cytokine signaling and functional outcomes.

Colon histopathology of colitis-induced BALB/c mice pretreated with mIL-2:JES6-1.

Spangler, J. B., Tomala, J., Luca, V. C., Jude, K. M., Dong, S., Ring, A. M., ... & Garcia, K. C. (2015). Antibodies to interleukin-2 elicit selective T cell subset potentiation through distinct conformational mechanisms. Immunity, 42(5), 815-825.

ELISA

Figure 6 Anti-IL-2 antibodies and IL-2 receptor compete for cytokine binding.

Yeast surface mIL-2 competition studies between anti-mIL-2 antibodies (Abs) and saturating concentrations of mIL-2Rα are shown (error bars, SD). Data are representative of three independent experiments.

Spangler, J. B., Tomala, J., Luca, V. C., Jude, K. M., Dong, S., Ring, A. M., ... & Garcia, K. C. (2015). Antibodies to interleukin-2 elicit selective T cell subset potentiation through distinct conformational mechanisms. Immunity, 42(5), 815-825.


Specifications

  • Immunogen
  • Mouse interleukin 2
  • Host Species
  • Mouse
  • Type
  • Mouse IgG
  • Specificity
  • Mouse Il2
  • Species Reactivity
  • Mouse
  • Clone
  • JES6-1
  • Applications
  • FC, FuncS, IHC, ELISA

Applications

  • Application Notes
  • This antibody has been reported for use in Flow Cytometry, Function Assay, Immunohistochemistry and Enzyme-Linked Immunosorbent Assay.

Target

  • Alternative Names
  • IL2; interleukin 2; Il-2; interleukin-2; TCGF; T-cell growth factor

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

See other products for "IL2"

See other products for "Clone JES6-1"


For Research Use Only. Not For Clinical Use.

* Abbreviations
3D IHC3D Immunohistochemistry
ActivActivation
AgonistAgonist
ApopApoptosis
BABioassay
BIBioimaging
BlockBlocking
Cell ScreeningCell Screening
SeparationCell Separation
ChIPChromatin Immunoprecipitation
CMCDComplement Mediated Cell Depletion
CostimCostimulation
CytCytotoxicity
DepletionDepletion
DBDot Blot
EMElectron Microscopy
ELISAEnzyme-linked Immunosorbent Assay
ELISPOTEnzyme-linked Immunosorbent Spot
FCFlow Cytometry
FuncSFunctional Assay
GSGel Super Shift Assay
HAHemagglutination
IAImmunoassay
IBImmunoblotting
ICCImmunocytochemistry
IDImmunodiffusion
IFImmunofluorescence
IHCImmunohistochemistry
IHC-FrImmunohistochemistry-Frozen
IHC-PImmunohistochemistry-Paraffin
REImmunohistology - Resin Sections
IPImmunoprecipitation
IRMAImmunoradiometric Assay
SHIn situ hybridization
InhibInhibition
ICFCIntracellular Staining for Flow Cytometry
KO/KD-WBKnockout/Knockdown target confirmation by Western Blot
Live cell imagingLive cell imaging
CyTOF®Mass Cytometry
MeDIPMethylated DNA Immunoprecipitation
MultiplexMultiplex bead-based assay
NeutNeutralization
PPProtein Purification
PGProteogenomics
RIRadial Immunodiffusion
RIARadioimmunoassay
StimStimulation
SPRSurface Plasmon Resonance
TCTissue Culture
TBTurbidimetry
WBWestern Blot

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