Mouse Anti-KIT Recombinant Antibody (clone 79D) (CAT#: PABL-642)
Recombinant Mouse Antibody (79D) is capable of binding to Kit. The inhibitory KIT antibodies that bind to the membrane-proximal Ig-like D4 of KIT with significant overlap with an epitope in D4 that mediates homotypic interactions essential for KIT activation.
Specific Inquiry
IB
Figure 1 WT KIT activation is inhibited by anti-D4 antibodies.
NIH 3T3 cells expressing WT KIT were incubated for 5 h with the indicated concentrations (Upper) of Fab79D following stimulation with 25 ng/mL of SCF for 5 minat 37 °C. Lysates of unstimulated or SCF-stimulated cells were subjected to immunoprecipitation (IP) with anti-KIT antibodies followed by SDS/PAGE and immunoblotting (IB) with anti-KIT or anti-phosphotyrosine (pY) anti-bodies.
Reshetnyak, A. V., Nelson, B., Shi, X., Boggon, T. J., Pavlenco, A., Mandel-Bausch, E. M., ... & Schlessinger, J. (2013). Structural basis for KIT receptor tyrosine kinase inhibition by antibodies targeting the D4 membrane-proximal region. Proceedings of the National Academy of Sciences, 110(44), 17832-17837.
Inhib
Figure 2 WT KIT activation is inhibited by anti-D4 antibodies.
Ba/F3 cells expressing WT KIT were plated in six-well platesand grown in the presence of 250 ng/mL SCF and different concentrations as indicated of Fab 79D.
Reshetnyak, A. V., Nelson, B., Shi, X., Boggon, T. J., Pavlenco, A., Mandel-Bausch, E. M., ... & Schlessinger, J. (2013). Structural basis for KIT receptor tyrosine kinase inhibition by antibodies targeting the D4 membrane-proximal region. Proceedings of the National Academy of Sciences, 110(44), 17832-17837.
Specifications
- Immunogen
- Human v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- Mouse IgG
- Specificity
- Tested positive against Kit
- Species Reactivity
- Human
- Clone
- 79D
- Applications
- WB, FuncS
Product Property
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term.
Target
- Alternative Names
- KIT; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog; PBT; SCFR; C-Kit; CD117; maststem cell growth factor receptor Kit; p145 c-kit; proto-oncogene c-Kit; piebald trait protein; soluble KIT variant 1; tyrosine-protein kinase Kit; proto-oncogene tyrosine-protein kinase Kit; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein
- Gene ID
- 3815
- UniProt ID
- A0A024RDA0
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-470CL | Human Anti-KIT Recombinant Antibody (TAB-470CL) | ELISA, WB | Human IgG |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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