Hi-Affi™ Recombinant Rabbit Anti-KIT Monoclonal Antibody (TH61DS)

Name: Hi-Affi™ Recombinant Rabbit Anti-KIT Monoclonal Antibody (TH61DS)
SKU: MOR-4551
Rating: 5 (1 reviews)

CAT#: MOR-4551

Recombinant Rabbit anti-KIT monoclonal antibody for WB, IF, ICC, excellent for producing superior results. Hi-Affi™ antibodies are developed from different sources, such as B cells of immunized animals, hybridoma, phage display library. The application of recombinant technology avoids the traditional ascitic fluid approach, which achieves the antibody generation in a humane way with much larger scale, greater specificity and sensitivity, and not susceptible to cell-line drift or lot-to-lot variation.

Gene Expression

Subcellular Location Figure 1 IF staining of human cell line HEK 293

Immunofluorescent staining of human cell line HEK 293 shows localization to plasma membrane.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/73252/1469_D7_6_selected.jpg

Normal Tissue Figure 2 High expression in breast

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4471/ihc_selected.jpg

Normal Tissue Figure 3 High expression in lung.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3288/8181_A_1_4_val_selected.jpg

Normal Tissue Figure 4 Skin

Melanocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3288/8181_B_8_1.jpg

RNA Expression Figure 5 RNA cell line category: Group enriched (HEL, HMC-1)

Cell lines ordered by descending RNA expression order

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000157404-KIT

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Specifications

Immunogen
proprietary

Host Species
Rabbit

Type
IgG

Species Reactivity
Human

Clone
TH61DS

Applications
Used for immunoassay techniques such as: Western Blot; Immunofluorescence; Immunocytochemistry

Conjugate
Unconjugated

Product Property

Purification
Purified with Absolute Mag™ Protein A Agarose

Purity
> 95% as determined by both RP-HPLC and SDS-PAGE analysis.

Format
Liquid

Buffer
PBS

Preservative
0.09% sodium azide

Storage
Store at -20°C for long-term storage. Store at 4°C for up to one month. Avoid freeze/thaw cycles.

Applications

Application Notes
ICC: 2-3 µg/ml; IF: 2-3 µg/ml; WB: 2-5 µg/ml

Target

Alternative Names
KIT Proto-Oncogene Receptor Tyrosine Kinase; V-Kit Hardy-Zuckerman 4 Feline Sarcoma Viral Oncogene Homolog; Tyrosine-Protein Kinase Kit; Piebald Trait Protein; Proto-Oncogene C-Kit; EC 2.7.10.1; P145 C-Kit; SCFR; PBT; V-Kit Hardy-Zuckerman 4 Feline Sarcoma Viral Oncogene-Like Protein; Proto-Oncogene Tyrosine-Protein Kinase Kit; Soluble KIT Variant 1; Piebald Trait; CD117 Antigen; EC 2.7.10; C-Kit; CD117

Gene ID
3815

UniProt ID
P10721

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Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - MOR-4551.pdf

MSDS

MSDS - MOR-4551.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

Lot Number:

Protocol & Troubleshooting

Immunofluorescence (IF)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

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