Human Anti-KIT scFv-Fc Chimera (VS-0325-FY38)
CAT#: VS-0325-FY38
The anti-KIT scFv-Fc antibody targets the KIT receptor tyrosine kinase, which is involved in cell proliferation and survival. It is engineered to inhibit KIT signaling, making it a potential therapeutic agent for cancers such as gastrointestinal stromal tumors (GIST) that are driven by KIT mutations. It serves as a valuable tool for researchers studying KIT signaling pathways and related diseases.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line HEK 293
Immunofluorescent staining of human cell line HEK 293 shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/73252/1469_D7_6_selected.jpg
Normal Tissue
Figure 2 High expression in breast
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4471/ihc_selected.jpg
Normal Tissue
Figure 3 High expression in lung.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3288/8181_A_1_4_val_selected.jpg
Normal Tissue
Figure 4 Skin
Melanocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3288/8181_B_8_1.jpg
RNA Expression
Figure 5 RNA cell line category: Group enriched (HEL, HMC-1)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000157404-KIT
❮
❯
Specifications
- Host Species
- Human
- Type
- Human IgG1, scFv-Fc
- Specificity
- Human KIT
- Species Reactivity
- Human
- Applications
- Inhibition, Flow Cytometry
- Related Disease
- Cancer
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Target
- Alternative Names
- KIT Proto-Oncogene Receptor Tyrosine Kinase; V-Kit Hardy-Zuckerman 4 Feline Sarcoma Viral Oncogene Homolog; Tyrosine-Protein Kinase Kit; Piebald Trait Protein; Proto-Oncogene C-Kit; EC 2.7.10.1; P145 C-Kit; SCFR; PBT; V-Kit Hardy-Zuckerman 4 Feline Sarcoma Viral Oncogene-Like Protein
- Gene ID
- 3815
- UniProt ID
- P10721
- Long Name
- KIT Proto-Oncogene Receptor Tyrosine Kinase
- Sequence Similarities
- Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
- Cellular Localization
- Cell membrane; Cytoplasm; Membrane
- Post Translation Modifications
- Ubiquitinated by SOCS6. KIT is rapidly ubiquitinated after autophosphorylation induced by KITLG/SCF binding, leading to internalization and degradation.
Autophosphorylated on tyrosine residues. KITLG/SCF binding enhances autophosphorylation. Isoform 1 shows low levels of tyrosine phosphorylation in the absence of added KITLG/SCF (in vitro). Kinase activity is down-regulated by phosphorylation on serine residues by protein kinase C family members. Phosphorylation at Tyr-568 is required for interaction with PTPN11/SHP-2, CRK (isoform Crk-II) and members of the SRC tyrosine-protein kinase family. Phosphorylation at Tyr-570 is required for interaction with PTPN6/SHP-1. Phosphorylation at Tyr-703, Tyr-823 and Tyr-936 is important for interaction with GRB2. Phosphorylation at Tyr-721 is important for interaction with PIK3R1. Phosphorylation at Tyr-823 and Tyr-936 is important for interaction with GRB7.
- Protein Refseq
- NP_000213.1; NP_001087241.1
- Function
- Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine KITLG/SCF and plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. In response to KITLG/SCF binding, KIT can activate several signaling pathways. Phosphorylates PIK3R1, PLCG1, SH2B2/APS and CBL. Activates the AKT1 signaling pathway by phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase. Activated KIT also transmits signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. Promotes activation of STAT family members STAT1, STAT3, STAT5A and STAT5B. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KIT signaling is modulated by protein phosphatases, and by rapid internalization and degradation of the receptor. Activated KIT promotes phosphorylation of the protein phosphatases PTPN6/SHP-1 and PTPRU, and of the transcription factors STAT1, STAT3, STAT5A and STAT5B. Promotes phosphorylation of PIK3R1, CBL, CRK (isoform Crk-II), LYN, MAPK1/ERK2 and/or MAPK3/ERK1, PLCG1, SRC and SHC1.
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for VS-0325-FY38. Click the button above to contact us or submit your feedback about this product.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "KIT"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-470CL | Human Anti-KIT Recombinant Antibody (TAB-470CL) | ELISA, WB | Human IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-450CL | Afuco™ Anti-KIT Recombinant Antibody (AFC-450CL), ADCC Enhanced | ELISA, WB | Humanized IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBL-255 | Human Anti-KIT Recombinant Antibody (clone Fab19); scFv Fragment | WB, ELISA, FuncS | Human scFv |
| HPAB-3398LY-S(P) | Human Anti-KIT Recombinant Antibody scFv Fragment (HPAB-3398LY-S(P)) | ELISA, FC, WB | Human scfv |
| HPAB-1570-FY-F(E) | Human Anti-KIT Recombinant Antibody; Fab Fragment (HPAB-1570-FY-F(E)) | WB, ELISA | Human Fab |
| HPAB-1571-FY-F(E) | Human Anti-KIT Recombinant Antibody; Fab Fragment (HPAB-1571-FY-F(E)) | WB, ELISA | Human Fab |
| HPAB-1572-FY-F(E) | Human Anti-KIT Recombinant Antibody; Fab Fragment (HPAB-1572-FY-F(E)) | WB, ELISA | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-465LC-S(P) | Human Anti-KIT Recombinant Antibody; scFv Fragment (TAB-465LC-S(P)) | FC, WB, SPR, ELISA | Human scFv |
| TAB-466LC-S(P) | Human Anti-KIT Recombinant Antibody; scFv Fragment (TAB-466LC-S(P)) | FC, WB, SPR, ELISA | Human scFv |
| TAB-465LC-F(E) | Human Anti-KIT Recombinant Antibody; Fab Fragment (TAB-465LC-F(E)) | FC, WB, SPR, ELISA | Human Fab |
| TAB-466LC-F(E) | Human Anti-KIT Recombinant Antibody; Fab Fragment (TAB-466LC-F(E)) | FC, WB, SPR, ELISA | Human Fab |
| TAB-468LC-F(E) | Human Anti-KIT Recombinant Antibody; Fab Fragment (TAB-468LC-F(E)) | FC, WB, ELISA | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0316MZ | Chicken Anti-KIT Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-1629CQ | Recombinant Mouse Anti-KIT Antibody (A3C6E2) | FC, BL | IgG1, κ |
| NEUT-1633CQ | Recombinant Rat Anti-Kit Antibody (ACK2) | FC, BL, Depletion | IgG2b, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-1630CQ | Recombinant Mouse Anti-KIT Antibody (CBL788) | WB, FC, CyTOF, ELISA(Cap), Neut | IgG1 |
| NEUT-1631CQ | Recombinant Mouse Anti-KIT Antibody (14G5) | Neut, WB | IgG1 |
| NEUT-1632CQ | Recombinant Mouse Anti-KIT Antibody (12A8) | WB, IP, FC, Neut | IgG1 |
| NEUT-1634CQ | Recombinant Mouse Anti-KIT Antibody (CBL158) | Neut, WB | IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1948 | Hi-Affi™ Recombinant Rabbit Anti-KIT Monoclonal Antibody (DS1948AB) | IHC-P, IHC-Fr | IgG |
| MOR-4551 | Hi-Affi™ Recombinant Rabbit Anti-KIT Monoclonal Antibody (TH61DS) | WB, IF, ICC | IgG |
| MOR-4552 | Hi-Affi™ Recombinant Rabbit Anti-KIT Monoclonal Antibody (TH62DS) | WB, IF, ICC, FC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0024-CN-F(E) | Mouse Anti-KIT Recombinant Antibody (clone Kit2C75); Fab Fragment | FC | Mouse Fab |
| HPAB-N0156-YC-F(E) | Mouse Anti-KIT Recombinant Antibody; Fab Fragment (HPAB-N0156-YC-F(E)) | ELISA, Inhib, FuncS | Mouse Fab |
| HPAB-N0157-YC-F(E) | Human Anti-KIT Recombinant Antibody; Fab Fragment (HPAB-N0157-YC-F(E)) | ELISA, Inhib, FuncS | Humanized Fab |
| HPAB-1947-FY-F(E) | Human Anti-KIT Recombinant Antibody; Fab Fragment (HPAB-1947-FY-F(E)) | Block, ELISA, FC, IF | Humanized Fab |
| HPAB-1948-FY-F(E) | Human Anti-KIT Recombinant Antibody; Fab Fragment (HPAB-1948-FY-F(E)) | Block, ELISA, FC, IF | Humanized Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-N0156-YC | Mouse Anti-KIT Recombinant Antibody (HPAB-N0156-YC) | ELISA, Inhib, FuncS | Mouse IgG |
| HPAB-N0157-YC | Human Anti-KIT Recombinant Antibody (HPAB-N0157-YC) | ELISA, Inhib, FuncS | Humanized IgG1 |
| ZG-0426F | Mouse Anti-KIT Recombinant Antibody (ZG-0426F) | WB, IHC, ELISA | Mouse IgG |
| ZG-0427F | Mouse Anti-KIT Recombinant Antibody (ZG-0427F) | WB, IHC, ELISA | Mouse IgG |
| VS3-FY795 | Recombinant Mouse Anti-KIT Antibody (clone 1H1B1) | ELISA, ICC, FC | Mouse IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-QX638 | Mouse Anti-KIT Recombinant Antibody (clone 566CT8.5.4) | WB, ICC | Mouse IgM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1349 | AbPlus™ Anti-Kit Magnetic Beads (VS-0724-YC1349) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0924-YC33 | Mouse Anti-KIT Recombinant Antibody (VS-0924-YC33) - Cancer Stem Cell Marker | IHC, WB | |
| VS-0924-YC34 | Rabbit Anti-KIT Antibody (VS-0924-YC34) - Cancer Stem Cell Marker | IHC, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY127 | Mouse Anti-NHP KIT Recombinant Antibody (clone 104D2) | FC | Mouse IgG1, kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0225-XY154 | CytoStream™ Mouse Anti-KIT Recombinant Antibody (clone 104D2) | FC | Mouse IgG1 |
| VS-0225-XY155 | CytoStream™ Rat Anti-KIT Recombinant Antibody (clone ACK45) | FC | Rat IgG2b, kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC461 | Recombinant Anti-KIT Vesicular Antibody, EV Displayed (VS-0425-YC461) | ELISA, FC, Inhib, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY3787 | Anti-KIT Immunohistochemistry Kit | IHC | |
| VS-0525-XY3788 | Anti-Mouse KIT Immunohistochemistry Kit | IHC |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: FC, IP, ELISA, Neut, FuncS, IF, WB
Application: IP, IF, FuncS, FC, Neut, ELISA, IHC
Application: IF, IP, Neut, FuncS, ELISA, FC, ICC
Application: ELISA, IP, FC, FuncS, Neut, IF, ICC
Application: Neut, ELISA, Inhib, ICC, WB
Application: Neut, FC, IHC-Fr, IP, BA
Application: FC, IHC-Fr, IP, ELISA, Block
Application: ELISA, IHC, FC, IP, IF, FuncS
Application: ELISA, WB, FC
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
