Rabbit Anti-KIT Antibody (VS-0924-YC34) - Cancer Stem Cell Marker (CAT#: VS-0924-YC34)

• Host Species
• Rabbit

• Type
• Rabbit IgG

• Specificity
• Human KIT

• Species Reactivity
• Human

• Clonality
• Polyclonal

• Purification
• Affinity purified using the PrEST antigen as affinity ligand

• Buffer
• PBS, pH 7.2, with 40% glycerol

• Preservative
• 0.02% Sodium azide

• Storage
• Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

• Alternative Names
• KIT Proto-Oncogene Receptor Tyrosine Kinase; V-Kit Hardy-Zuckerman 4 Feline Sarcoma Viral Oncogene Homolog; Tyrosine-Protein Kinase Kit; Piebald Trait Protein; Proto-Oncogene C-Kit; EC 2.7.10.1; P145 C-Kit; SCFR; PBT; V-Kit Hardy-Zuckerman 4 Feline Sarcoma Viral Oncogene-Like Protein

• Gene ID
• 3815

• UniProt ID
• P10721

• Long Name
• v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog

• Sequence Similarities
• Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.

• Cellular Localization
• Cell membrane, Cytoplasm, Membrane

• Post Translation Modifications
• Ubiquitinated by SOCS6. KIT is rapidly ubiquitinated after autophosphorylation induced by KITLG/SCF binding, leading to internalization and degradation.
  Autophosphorylated on tyrosine residues. KITLG/SCF binding enhances autophosphorylation. Isoform 1 shows low levels of tyrosine phosphorylation in the absence of added KITLG/SCF (in vitro). Kinase activity is down-regulated by phosphorylation on serine residues by protein kinase C family members. Phosphorylation at Tyr-568 is required for interaction with PTPN11/SHP-2, CRK (isoform Crk-II) and members of the SRC tyrosine-protein kinase family. Phosphorylation at Tyr-570 is required for interaction with PTPN6/SHP-1. Phosphorylation at Tyr-703, Tyr-823 and Tyr-936 is important for interaction with GRB2. Phosphorylation at Tyr-721 is important for interaction with PIK3R1. Phosphorylation at Tyr-823 and Tyr-936 is important for interaction with GRB7.

• Protein Refseq
• NP_000213.1; NP_001087241.1

• Function
• Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine KITLG/SCF and plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. In response to KITLG/SCF binding, KIT can activate several signaling pathways. Phosphorylates PIK3R1, PLCG1, SH2B2/APS and CBL. Activates the AKT1 signaling pathway by phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase. Activated KIT also transmits signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. Promotes activation of STAT family members STAT1, STAT3, STAT5A and STAT5B. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KIT signaling is modulated by protein phosphatases, and by rapid internalization and degradation of the receptor. Activated KIT promotes phosphorylation of the protein phosphatases PTPN6/SHP-1 and PTPRU, and of the transcription factors STAT1, STAT3, STAT5A and STAT5B. Promotes phosphorylation of PIK3R1, CBL, CRK (isoform Crk-II), LYN, MAPK1/ERK2 and/or MAPK3/ERK1, PLCG1, SRC and SHC1.

Isotype Control

• MOB-208CQ
• Rabbit IgG Isotype Control Antibody

Secondary Antibody

• 2ND-061CQ
• Sheep Anti-Rabbit IgG (H&L) Antibody

• MOB-0264MC
• Goat Anti-Rabbit IgG Antibody (HRP)

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