Human Anti-KIT Recombinant Antibody (clone Fab19) (CAT#: PABL-255)
Recombinant Human Antibody (Fab19) is capable of binding to Kit, expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-Kit mAb and CH1-3 region of human IgG1 and a light chain (LC) encoding VL from anti-Kit mAb and CL of human light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition.
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Figure 1 NIH 3T3 cells expressing WT KIT were incubated for 5 h with the indicated concentrations (Upper) of Fabs or IgGs following stimulation with 25 ng/mL of SCF for 5 min at 37℃. Lysates of unstimulated or SCF-stimulated cells were subjected to immunoprecipitation (IP) with anti-KIT antibodies followed by SDS/PAGE and immunoblotting (IB) with anti-KIT or anti-phosphotyrosine (pY) antibodies.
Reshetnyak, A. V., Nelson, B., Shi, X., Boggon, T. J., Pavlenco, A., Mandel-Bausch, E. M.,... & Schlessinger, J. (2013). Structural basis for KIT receptor tyrosine kinase inhibition by antibodies targeting the D4 membrane-proximal region. Proceedings of the National Academy of Sciences,110(44), 17832-17837.
Figure 2 (B and C) Ba/F3 cells expressing WT KIT were plated in six-well plates and grown in the presence of 250 ng/mL SCF and different concentrations as indicated (Lower) of Fabs (B) or IgGs (C). Cell numbers were measured after 72 h relative to 0 h time points.
Reshetnyak, A. V., Nelson, B., Shi, X., Boggon, T. J., Pavlenco, A., Mandel-Bausch, E. M.,... & Schlessinger, J. (2013). Structural basis for KIT receptor tyrosine kinase inhibition by antibodies targeting the D4 membrane-proximal region. Proceedings of the National Academy of Sciences,110(44), 17832-17837.
Figure 3 Proliferation of Ba/F3 cells expressing A502,Y503 duplication oncogenic KIT mutant is inhibited by anti-D4 antibodies. Ba/F3 cells expressing the dupA502,Y503 KIT mutant were plated in 6 well plates. Cells were grown in the presence of different concentrations of Fabs (A) or IgGs (B), in the absence of IL-3 or SCF (as indicated). Folds increase of cell number was measured after 72 hours.
Specifications
- Immunogen
- Human v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog
- Host Species
- Human
- Derivation
- Human
- Type
- Human IgG
- Specificity
- Human KIT
- Species Reactivity
- Human
- Clone
- Fab19
- Applications
- WB, ELISA, FuncS
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- KIT; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog; PBT; SCFR; C-Kit; CD117; mast/stem cell growth factor receptor Kit; p145 c-kit; proto-oncogene c-Kit; piebald trait protein; soluble KIT variant 1; tyrosine-protein kinase Kit; proto-oncogene tyrosine-protein kinase Kit; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein
- Gene ID
- 3815
- UniProt ID
- A0A024RDA0
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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IHC Kits & Antibodies
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-470CL | Human Anti-KIT Recombinant Antibody (TAB-470CL) | ELISA, WB | Human IgG |
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