Human Anti-KIT Recombinant Antibody (clone Fab19) (CAT#: PABL-255)
Recombinant Human Antibody (Fab19) is capable of binding to Kit, expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-Kit mAb and CH1-3 region of human IgG1 and a light chain (LC) encoding VL from anti-Kit mAb and CL of human light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition.
Specific Inquiry
Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
Figure 1 NIH 3T3 cells expressing WT KIT were incubated for 5 h with the indicated concentrations (Upper) of Fabs or IgGs following stimulation with 25 ng/mL of SCF for 5 min at 37℃. Lysates of unstimulated or SCF-stimulated cells were subjected to immunoprecipitation (IP) with anti-KIT antibodies followed by SDS/PAGE and immunoblotting (IB) with anti-KIT or anti-phosphotyrosine (pY) antibodies.
Reshetnyak, A. V., Nelson, B., Shi, X., Boggon, T. J., Pavlenco, A., Mandel-Bausch, E. M.,... & Schlessinger, J. (2013). Structural basis for KIT receptor tyrosine kinase inhibition by antibodies targeting the D4 membrane-proximal region. Proceedings of the National Academy of Sciences,110(44), 17832-17837.
Figure 2 (B and C) Ba/F3 cells expressing WT KIT were plated in six-well plates and grown in the presence of 250 ng/mL SCF and different concentrations as indicated (Lower) of Fabs (B) or IgGs (C). Cell numbers were measured after 72 h relative to 0 h time points.
Reshetnyak, A. V., Nelson, B., Shi, X., Boggon, T. J., Pavlenco, A., Mandel-Bausch, E. M.,... & Schlessinger, J. (2013). Structural basis for KIT receptor tyrosine kinase inhibition by antibodies targeting the D4 membrane-proximal region. Proceedings of the National Academy of Sciences,110(44), 17832-17837.
Figure 3 Proliferation of Ba/F3 cells expressing A502,Y503 duplication oncogenic KIT mutant is inhibited by anti-D4 antibodies. Ba/F3 cells expressing the dupA502,Y503 KIT mutant were plated in 6 well plates. Cells were grown in the presence of different concentrations of Fabs (A) or IgGs (B), in the absence of IL-3 or SCF (as indicated). Folds increase of cell number was measured after 72 hours.
Specifications
- Immunogen
- Human v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog
- Host Species
- Human
- Derivation
- Human
- Type
- Human IgG
- Specificity
- Human KIT
- Species Reactivity
- Human
- Clone
- Fab19
- Applications
- WB, ELISA, FuncS
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- KIT; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog; PBT; SCFR; C-Kit; CD117; mast/stem cell growth factor receptor Kit; p145 c-kit; proto-oncogene c-Kit; piebald trait protein; soluble KIT variant 1; tyrosine-protein kinase Kit; proto-oncogene tyrosine-protein kinase Kit; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein
- Gene ID
- 3815
- UniProt ID
- A0A024RDA0
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "Clone Fab19"
See other products for "KIT"
Select a product category from the dropdown menu below to view related products.
Please select product type
Humanized Antibody Products
ADCC Enhanced Antibody Products
IgG Antibody Products
Fab Antibody Products
Human Antibody Products
Mouse Antibody Products
Chicken IgY Antibody Products
Blocking Antibody Products
Neutralizing Antibody Products
Rabbit Monoclonal Antibody Products
ScFv Antibody Products
IgM Antibody Products
Antibody Magnetic Beads
Cancer Stem Cell Biomarker Specific Antibody Products
Non-Human Primate (NHP) Antibody Products
Flow Cytometry (FC) related Reagents and Kits: Empowering Cell Function Research
ScFv-Fc Chimera Products
Extracellular Vesicle (EV) Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-470CL | Human Anti-KIT Recombinant Antibody (TAB-470CL) | ELISA, WB | Human IgG |
Customer Reviews and Q&As
There are currently no Customer reviews or questions for PABL-255. Click the button above to contact us or submit your feedback about this product.
Popular products with customers
Application: IF, IP, Neut, FuncS, ELISA, FC, ICC
Application: IF, IP, Neut, FuncS, ELISA, FC, ICC
Application: WB, FC, IP, ELISA, Neut, FuncS, IF
Application: Neut, ELISA, IF, IP, FuncS, FC, ICC
Application: IF, IP, Neut, FuncS, ELISA, FC, ICC
Application: FuncS, Inhib, IP, ELISA
-3-1.png)
Application: ELISA, FC, WB, Block
Application: Neut, FC
Application: ELISA, IHC, FC, IP, IF, FuncS
Application: ELISA, IHC, FC, IP, IF, FuncS
Application: Neut, ELISA, IF, IP, FuncS, FC
For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
