Human Anti-MAPT Recombinant Antibody (clone pT231/pS235_1) (CAT#: PABZ-196)
Recombinant Chimeric (Chicken/Human) Antibody (pT231/pS235_1) is capable of binding to MAPT. The mAb exhibits novel complementarity determining region (CDR) structures with a "bowl-like" conformation in CDR-H2 that tightly and specifically interacts with the phospho-Thr-231 phosphate group, as well as a long, disulfide-constrained CDR-H3 that mediates peptide recognition.
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Figure 1 Expression and binding activity of chimeric IgG molecules converted from scFv.
A, Western blot analysis of chimeric IgG molecules converted from scFv clones pT231/pS235_1, pS396/pS404_1, and pT212/pS214_1, respectively. The heavy and light chains for each IgG are indicated by arrows. B–D, ELISA analysis of each purified IgG molecule for binding to the phosphopeptide, non-phosphopeptide, and scrambled (Scr) peptide.
Shih, H. H., Tu, C., Cao, W., Klein, A., Ramsey, R., Fennell, B. J., ... & Laxmanan, S. (2012). An ultra-specific avian antibody to phosphorylated tau protein reveals a unique mechanism for phosphoepitope recognition. Journal of Biological Chemistry, 287(53), 44425-44434.
Figure 2 Surface plasmon resonance kinetic analyses of anti-phospho-tau IgGs for phosphopeptides
Shih, H. H., Tu, C., Cao, W., Klein, A., Ramsey, R., Fennell, B. J., ... & Laxmanan, S. (2012). An ultra-specific avian antibody to phosphorylated tau protein reveals a unique mechanism for phosphoepitope recognition. Journal of Biological Chemistry, 287(53), 44425-44434.
Figure 3 Binding of pathological phospho-tau by antibody pT231/pS235_1 in human AD and Tg4510 transgenic mouse brains.
A–C, immunohistochemical staining of human AD brain (A and B) and healthy brain (C). B, magnification of the boxed area in A. D, Western blot analysis of Tg4510 transgenic and wild-type brain lysates with pT231/pS235_1 IgG (plus control anti-GAPDH antibody). Each lane represents a different animal, and the lysate samples are 3-month-old wild-type mice (lanes 1 and 2), 3-month-old transgenic mice (lanes 3 and 4), 6-month-old wild-type mice (lanes 5 and 6), and 6-month-old transgenic mice (lanes 7 and 8).
Shih, H. H., Tu, C., Cao, W., Klein, A., Ramsey, R., Fennell, B. J., ... & Laxmanan, S. (2012). An ultra-specific avian antibody to phosphorylated tau protein reveals a unique mechanism for phosphoepitope recognition. Journal of Biological Chemistry, 287(53), 44425-44434.
Specifications
- Immunogen
- Human microtubule-associated protein tau
- Host Species
- Human
- Derivation
- Chimeric (chicken/human)
- Type
- Chimeric (chicken/human) IgG
- Specificity
- Human MAPT
- Species Reactivity
- Human
- Clone
- pT231/pS235_1
- Applications
- ELISA, WB
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- MAPT; microtubule-associated protein tau; TAU; MSTD; PPND; DDPAC; MAPTL; MTBT1; MTBT2; FTDP-17; PPP1R103; PHF-tau; paired helical filament-tau; neurofibrillary tangle protein; microtubule-associated protein tau, isoform 4; protein phosphatase 1, regulatory subunit 103; G protein beta1/gamma2 subunit-interacting factor 1;
- Gene ID
- 4137
- UniProt ID
- P10636
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABL-305 | Chicken Anti-MAPT Recombinant Antibody | WB, ELISA, FuncS | Chicken IgY |
| MRO-2389-CN | Recombinant Rabbit Anti-MAPT (phosphorylated Ser396) Monoclonal Antibody (CBACN-674) | WB, IF, IHC | Rabbit IgG |
| MRO-2390-CN | Recombinant Rabbit Anti-MAPT (phosphorylated Thr231) Monoclonal Antibody (CBACN-675) | WB, IHC, IP | Rabbit IgG |
| HPAB-0196-YJ | Mouse Anti-MAPT Recombinant Antibody (HPAB-0196-YJ) | ELISA | Mouse IgG1 |
| HPAB-0197-YJ | Mouse Anti-MAPT Recombinant Antibody (HPAB-0197-YJ) | ELISA | Mouse IgG1 |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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