Human Anti-MAPT Recombinant Antibody (clone pT231/pS235_1)
CAT#: PABZ-196
Recombinant Chimeric (Chicken/Human) Antibody (pT231/pS235_1) is capable of binding to MAPT. The mAb exhibits novel complementarity determining region (CDR) structures with a "bowl-like" conformation in CDR-H2 that tightly and specifically interacts with the phospho-Thr-231 phosphate group, as well as a long, disulfide-constrained CDR-H3 that mediates peptide recognition.
Published Data
Gene Expression
Figure 1 Expression and binding activity of chimeric IgG molecules converted from scFv.
A, Western blot analysis of chimeric IgG molecules converted from scFv clones pT231/pS235_1, pS396/pS404_1, and pT212/pS214_1, respectively. The heavy and light chains for each IgG are indicated by arrows. B–D, ELISA analysis of each purified IgG molecule for binding to the phosphopeptide, non-phosphopeptide, and scrambled (Scr) peptide.
Shih, H. H., Tu, C., Cao, W., Klein, A., Ramsey, R., Fennell, B. J., ... & Laxmanan, S. (2012). An ultra-specific avian antibody to phosphorylated tau protein reveals a unique mechanism for phosphoepitope recognition. Journal of Biological Chemistry, 287(53), 44425-44434.
Figure 2 Surface plasmon resonance kinetic analyses of anti-phospho-tau IgGs for phosphopeptides
Shih, H. H., Tu, C., Cao, W., Klein, A., Ramsey, R., Fennell, B. J., ... & Laxmanan, S. (2012). An ultra-specific avian antibody to phosphorylated tau protein reveals a unique mechanism for phosphoepitope recognition. Journal of Biological Chemistry, 287(53), 44425-44434.
Figure 3 Binding of pathological phospho-tau by antibody pT231/pS235_1 in human AD and Tg4510 transgenic mouse brains.
A–C, immunohistochemical staining of human AD brain (A and B) and healthy brain (C). B, magnification of the boxed area in A. D, Western blot analysis of Tg4510 transgenic and wild-type brain lysates with pT231/pS235_1 IgG (plus control anti-GAPDH antibody). Each lane represents a different animal, and the lysate samples are 3-month-old wild-type mice (lanes 1 and 2), 3-month-old transgenic mice (lanes 3 and 4), 6-month-old wild-type mice (lanes 5 and 6), and 6-month-old transgenic mice (lanes 7 and 8).
Shih, H. H., Tu, C., Cao, W., Klein, A., Ramsey, R., Fennell, B. J., ... & Laxmanan, S. (2012). An ultra-specific avian antibody to phosphorylated tau protein reveals a unique mechanism for phosphoepitope recognition. Journal of Biological Chemistry, 287(53), 44425-44434.
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Subcellular Location
Figure 1 IF staining of human cell line BJ
Immunofluorescent staining of human cell line MCF7 shows localization to nuclear speckles & plasma membrane.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/48895/1070_E6_2_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line BJ
Immunohistochemical staining of human appendix shows strong positivity in peripheral ganglia and nerve fibers.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69524/156431_A_3_2_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Neuropil
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/151/156500_B_8_5.jpg
Normal Tissue
Figure 4 Colon
Endocrine cells
Staining:
Medium
Intensity: Moderate
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/151/156500_A_7_3.jpg
Normal Tissue
Figure 5 Kidney
Proximal tubules (cell body)
Staining:
Medium
Intensity: Moderate
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/151/156500_A_7_5.jpg
RNA Expression
Figure 6 RNA cell line category: Group enriched (HDLM-2, SCLC-21H, T-47d)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000186868-MAPT
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Specifications
- Immunogen
- Human microtubule-associated protein tau
- Host Species
- Human
- Derivation
- Chimeric (chicken/human)
- Type
- Chimeric (chicken/human) IgG
- Specificity
- Human MAPT
- Species Reactivity
- Human
- Clone
- pT231/pS235_1
- Applications
- ELISA, WB
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- MAPT; microtubule-associated protein tau; TAU; MSTD; PPND; DDPAC; MAPTL; MTBT1; MTBT2; FTDP-17; PPP1R103; PHF-tau; paired helical filament-tau; neurofibrillary tangle protein; microtubule-associated protein tau, isoform 4; protein phosphatase 1, regulatory subunit 103; G protein beta1/gamma2 subunit-interacting factor 1;
- Gene ID
- 4137
- UniProt ID
- P10636
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "Clone pT231/pS235_1"
- CAT
- Product Name
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBL-674 | Chicken Anti-MAPT Recombinant Antibody (clone pT231/pS235_1); Fab Fragment | WB, ELISA, FACS, Block | Chicken Fab |
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| CAT | Product Name | Application | Type |
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| NS-090CN-S(P) | Mouse Anti-MAPT Recombinant Antibody; scFv Fragment (NS-090CN-S(P)) | WB, ELISA, IHC, IP | Mouse scFv |
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| VS-0525-XY4255 | Anti-MAPT Immunohistochemistry Kit | IHC | |
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