Recombinant Human Antibody (5D5) is capable of binding to MET, expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-MET mAb and CH1-3 region of human IgG1 and a light chain (LC) encoding VL from anti-MET mAb and CL of human light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition. This antibody potently inhibits HGF binding and receptor phosphorylation and signaling and has antibody-like pharmacokinetics and antitumor activity.
Figure 1 MKN45 cells treated with 5 μg/ml of F46 or 5D5 were incubated for 24 h. c-Met degradation was measured by Western blot (A) and ELISA (B). Phosphorylation of c-Met and Akt in Caki-1 cells was measured by Western blot (C, E) and ELISA (D, F). Caki-1 cells were incubated for 30 min with 5 μg/ml of F46 or 5D5 or 100 ng/ml of HGF. The error bars indicate the mean ± SD of three independent experiments. Phosphorylation of c-Met downstream kinases in Caki-1 (G) and NCI-H441 (I) cells were measured by Western blot. Co-immunoprecipitation experiment showed induced binding between Gab1 and c-Met by 5D5, but not by F46 (H).
Figure 2 MKN45 cells were incubated with increasing amounts of recombinant human HGF. They were added with 0.5 μg of 5D5 or F46 followed by flow cytometry. ΔMFI (y-axis) indicates the decrease in percentage of mean fluorescence intensity (MFI) upon treatment with F46 or 5D5 (top). For the competition ELISA, biotinylated HGF and differing concentrations of IgG, F46, or free HGF were treated on c-Met coated plate (bottom).
Figure 3 A549, H441, and MDA-MB-435 cells were kept serum-free and treated with 10 μg/ml rSema or anti-Met 5D5-Fab.
The samples were immunoblotted with phospho-tyrosine, Met, phospho-MAPK, or MAPK antibodies.
Figure 4 293 cells were transfected with the indicated constructs and analyzed by 4%–12% SDS-PAGE. Lysates were immunoprecipitated with anti-Met 5D5 antibody and immunoblotted with His antibody (top) and reprobed with Met C-12 antibody (bottom).
Figure 5 rSema and anti-Met 5D5-Fab inhibit cell motility.
A: H441 cells were scraped on Day 0 and treated with increasing concentrations of rSema plus HGF in serum-free media. The scrapes were photographed on Day 0 and Day 1. A representative data set of three independent experiments is shown. B: H441 cells were scraped and treated with mock, rSema, or anti-Met 5D5-Fab in serum-free media over the course of 2 days. Photographs were taken on Day 0 and Day 2.
Figure 6 Cell migration is inhibited by rSema and anti-Met 5D5-Fab.
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|TAB-H24||Anti-Human MET Recombinant Antibody (Emibetuzumab)||FuncS, IF, Neut, ELISA, FC, IP, WB||IgG4 - kappa|
|TAB-521CL||Anti-Human MET Recombinant Antibody (ABT-700)||WB, FuncS||Antibody|
|TAB-0332CL||Human Anti-MET Recombinant Antibody (TAB-0332CL)||ELISA||Humanized Antibody|
|TAB-0332CL-F(E)||Human Anti-MET Recombinant Antibody; Fab Fragment (TAB-0332CL-F(E))||ELISA||Humanized Fab|
|TAB-026MZ||Anti-Human MET Recombinant Antibody (73R009)||WB||Humanized antibody|
|PSBL-271||Human Anti-MET Recombinant Antibody (clone 5D5); scFv Fragment||WB, Block, FuncS||Human scFv|
|PSBC-457||Human Anti-MET Recombinant Antibody (clone 107_A07); scFv Fragment||ELISA, Block||Human scFv|
|HPAB-0117-WJ-S(P)||Mouse Anti-MET Recombinant Antibody; scFv Fragment (HPAB-0117-WJ-S(P))||ELISA, WB||Mouse scFv|
|HPAB-0394-YJ-S(P)||Human Anti-MET Recombinant Antibody (clone S1); scFv Fragment||ELISA, WB, IP, FC||Human scFv|
|HPAB-0487-FY-S(P)||Human Anti-MET Recombinant Antibody; scFv Fragment (HPAB-0487-FY-S(P))||Inhib||Human scFv|
|PABZ-155||Afuco™ Anti-MET ADCC Recombinant Antibody (Onartuzumab), ADCC Enhanced||ELISA, Neut||ADCC enhanced antibody|
|AFC-TAB-H24||Afuco™ Anti-MET ADCC Recombinant Antibody (Emibetuzumab), ADCC Enhanced||FuncS, IF, Neut, ELISA, FC, IP||ADCC enhanced antibody|
|TAB-0530CL||Anti-Human MET Recombinant Antibody||ELISA, FC, IF, FuncS|
|TAB-536LC-F(E)||Human Anti-MET Recombinant Antibody; Fab Fragment (TAB-536LC-F(E))||ELISA||Human Fab|
|TAB-537LC-F(E)||Human Anti-MET Recombinant Antibody; Fab Fragment (TAB-537LC-F(E))||ELISA||Human Fab|
|TAB-538LC-F(E)||Human Anti-MET Recombinant Antibody; Fab Fragment (TAB-538LC-F(E))||ELISA||Human Fab|
|TAB-539LC-F(E)||Human Anti-MET Recombinant Antibody; Fab Fragment (TAB-539LC-F(E))||ELISA||Human Fab|
|TAB-002MZ||Anti-Human MET Recombinant Antibody (MvDN30)||IHC, PET imaging||Chimeric antibody (mouse/human)|
|TAB-002MZ-S(P)||Anti-Human MET Recombinant Antibody scFv Fragment (MvDN30)||IHC, PET imaging||Chimeric antibody (mouse/human)|
|TAB-0880CLV||Human Anti-MET Recombinant Antibody (TAB-0880CLV)||ELISA, FuncS||Chimeric (mouse/human) IgG1|
|TAB-0880CLV-F(E)||Human Anti-MET Recombinant Antibody; Fab Fragment (TAB-0880CLV-F(E))||ELISA, FuncS||Chimeric (mouse/human) Fab|
|TAB-0880CLV-S(P)||Mouse Anti-MET Recombinant Antibody; scFv Fragment (TAB-0880CLV-S(P))||ELISA, FuncS||Mouse scFv|
|TAB-011MZ||Mouse Anti-MET Recombinant Antibody (TAB-011MZ)||ELISA, WB, FC||Mouse IgG|
|TAB-012MZ||Mouse Anti-MET Recombinant Antibody (TAB-012MZ)||ELISA, WB, FC||Mouse IgG|
|TAB-027MZ||Mouse Anti-MET Recombinant Antibody (TAB-027MZ)||WB, IP, IF||Mouse lgG1|
|TAB-028MZ||Mouse Anti-MET Recombinant Antibody (TAB-028MZ)||IP||Mouse lgG1|
|TAB-029MZ||Mouse Anti-MET Recombinant Antibody (TAB-029MZ)||IP, WB||Mouse lgG1|
|MOB-1226CT||Recombinant Mouse anti-Human MET Monoclonal antibody (9G22)||IHC-Fr, IHC-P|
|HPAB-0071-YC||Mouse Anti-MET Recombinant Antibody (HPAB-0071-YC)||ELISA, Inhib||Mouse IgG|
|HPAB-0072-YC||Human Anti-MET Recombinant Antibody (HPAB-0072-YC)||ELISA||Humanized IgG1, κ|
|HPAB-0073-YC||Human Anti-MET Recombinant Antibody (HPAB-0073-YC)||ELISA||Human IgG1, κ|
|HPAB-0077-YC||Mouse Anti-MET Recombinant Antibody (clone LMH 89)||ELISA, FC, Stim, FuncS||Mouse IgG|
|MOR-2216||Hi-Affi™ Recombinant Rabbit Anti-MET Monoclonal Antibody (DS2216AB)||ICC/IF, IHC-P, WB||IgG|
|MOR-4519||Hi-Affi™ Recombinant Rabbit Anti-MET Monoclonal Antibody (TH27DS)||WB, IF, ICC||IgG|
|MOR-4645||Hi-Affi™ Recombinant Rabbit Anti-MET Monoclonal Antibody (TH158DS)||WB, IF, ICC, FC, ELISA||IgG|
|MOR-4694||Hi-Affi™ Recombinant Rabbit Anti-MET Monoclonal Antibody (TH208DS)||WB, ELISA||IgG|
|HPAB-0071-YC-F(E)||Mouse Anti-MET Recombinant Antibody; Fab Fragment (HPAB-0071-YC-F(E))||ELISA||Mouse Fab|
|HPAB-0072-YC-F(E)||Human Anti-MET Recombinant Antibody; Fab Fragment (HPAB-0072-YC-F(E))||ELISA||Humanized Fab|
|HPAB-0073-YC-F(E)||Human Anti-MET Recombinant Antibody; Fab Fragment (HPAB-0073-YC-F(E))||ELISA||Human Fab|
|HPAB-0077-YC-F(E)||Mouse Anti-MET Recombinant Antibody (clone LMH 89); Fab Fragment||ELISA, FC, Stim, FuncS||Mouse Fab|
|HPAB-1298LY-F(E)||Human Anti-MET Recombinant Antibody; Fab Fragment (HPAB-1298LY-F(E))||ELISA, IP||Human Fab|
|MHC-YF474||A*02:01/Human Hepatocyte growth factor receptor (YVDPVITSI) MHC Pentamer||FCM|
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.