Mouse Anti-MET Recombinant Antibody (clone LMH 89) (CAT#: HPAB-0077-YC)
The antibody clone LMH 89 specifically binds to unprocessed c-Met present on the surface of tumor cells. The epitope of LMH 89 is located in the region 236 to 245 of c-Met. The antibody may also be used in therapy study in combination with chemotherapeutics or anti-cancer agents.
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
Figure 1 Representative data showing characterization of selected LMH antibodies.
(A) flow cytometry analysis showing that LMH 85 and LMH 87 bind surface c-MET expressed on A549 cells. (B) western blots of representative LMH antibodies. c-MET was IPed with LMH 85 and membranes probed with indicated antibodies. The commercial 25H2 recognized the 170 kDa c-MET precursor (p170 c-MET; contains both α and β-chain) and the c-MET β-chain. Using this technique, LMH 80 only weakly bound the β-chain, while LMH 82, 84 and 87 bound p170 c-MET and α-chain. (C) IP with selected LMH antibodies. Following IP with different LMH antibodies, membranes were blotted with mAb 25H2. Antibodies shown were all capable of IP but LMH 80, LMH 81 and LMH 82 appeared specific for p170 c-MET using this technique. (D) biochemical activity of selected LMH antibodies. A459 cells were treated with antibody alone or antibody in the presence of HGF/SF and WCL were probed for total c-MET (upper panels) or phosphorylated c-MET (Y1234/Y1235) (lower panels). LMH 85 stimulated c-MET phosphorylation, whilst LMH 86 and LMH 87 did not. LMH 86 was unable to block HGF/SF stimulated c-MET phosphorylation. (E) effect of selected LMH antibodies on cell migration. Antibodies were tested for their ability to induce cell migration in SK-OV-3 cells. LMH 85 stimulated cell migration while LMH 87 had no effect (upper panel). Differing concentrations of LMH 87 were mixed with 3×10−10 M HGF/SF to determine if it inhibited the HGF/SF induced migration of SK-OV-3 cells. LMH 87 substantially inhibited the migratory activity stimulated by HGF/SF (lower panel).
Greenall, S. A., Gherardi, E., Liu, Z., Donoghue, J. F., Vitali, A. A., Li, Q.,... & Johns, T. G. (2012). Non-agonistic bivalent antibodies that promote c-MET degradation and inhibit tumor growth and others specific for tumor related c-MET. PloS one, 7(4), e34658.
Specifications
- Immunogen
- The human lung carcinoma cell line A549 and a purified truncated c-Met fragment designated 25-567H
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human MET
- Species Reactivity
- Human
- Clone
- LMH 89
- Applications
- ELISA, FC, Stim, FuncS
- Related Disease
- Cancer
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Flow Cytometry. For details, refer to Published Data.
Target
Related Resources
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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CAT | Product Name | Application | Type |
---|---|---|---|
AGTO-G049E | Anti-MET immunotoxin (Fab)-PE | Cytotoxicity assay, Function study |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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