Mouse Anti-SNCA Recombinant Antibody (TAB-0748CLV) (CAT#: TAB-0748CLV)
This product is a recombinant mouse antibody that recognizes aggregated forms of α-synuclein. This antibody could be used in immunoassays such as ELISA, DB, and WB. The antibody was expressed in mammalian cells with chemically defined culture media and was purified by affinity chromatography. This antibody product could be used in the research of detection, prevention, and immunotherapies of neuropathologies associated with α-synuclein.
Specific Inquiry
WB
Figure 1 Anti-Human SNCA Antibody (TAB-0748CLV) in WB
Western blot analysis of TAB-0748CLV was performed with alpha-Synuclein Protein, Human, Recombinant.
TAB-0748CLV incubation concentration: 2ng/μL.
The secondary antibody: HRP-Anti-Mouse IgG (H+L)
Lane 1: Reducing Antigen (0.1μg)
Lane 2: Reducing Antigen (0.3μg)
Lane 3: Reducing Antigen (0.6μg)
ELISA
Figure 2 Anti-Human SNCA Antibody (TAB-0748CLV) in ELISA
ELISA analysis of TAB-0748CLV was performed by coating with alpha-Synuclein Protein, Human, Recombinant.
The secondary antibody: HRP-Anti-Mouse IgG (H+L)
Specifications
- Immunogen
- α-synuclein oligomers
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human SNCA
- Species Reactivity
- Human
- Applications
- ELISA, DB, WB
- Related Disease
- Parkinson's disease, dementia with Lewy bodies, multiple system atrophy
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- SNCA; synuclein, alpha; PD1; NACP; PARK1; PARK4; Alpha-synuclei
- Gene ID
- 6622
- UniProt ID
- P37840
Related Resources
Helwig, Michael, et al. "Neuronal hyperactivity-induced oxidant stress promotes in vivo α-synuclein brain spreading." Science advances 8.35 (2022): eabn0356.
This study investigated the effects of neuronal hyperactivity on the spreading of the Parkinson's disease-associated protein α-synuclein (αS) in the brain. Using a mouse model, researchers found that increased neuronal activity exacerbated oxidative and nitrative stress, leading to enhanced accumulation and aggregation of nitrated αS. The experiments demonstrated that hyperactivity significantly promoted the interneuronal transfer and brain-wide distribution of αS, suggesting a mechanistic role of oxidant stress in facilitating the protein's spread. The study employed advanced genetic and viral techniques to modulate neuronal activity and track the movement of αS across different brain regions, providing valuable insights into the pathophysiological processes underlying neurodegenerative diseases.
Creative Biolabs played a crucial role in this research by supplying key reagents. They provided the Syn-O2 antibody (Cat#: TAB-0748CLV) used for double fluorescence labeling. This involved incubating sections with anti-RFP or mouse anti-Syn-O2, followed by anti-h-αS, and labeling with secondary antibodies conjugated with DyLight 488 or DyLight 594. These reagents were essential for the immunofluorescence analysis, enabling precise detection and visualization of protein interactions and modifications within the study.
Simon, Isak. "Expression & affinity analysis of recombinant RX against pathogenic α-synuclein." (2021).
This study investigated the potential of recombinant RX protein constructs as a therapeutic approach to counteract the pathogenesis of Parkinson's disease by targeting the neurotoxic oligomers of α-synuclein. The research aimed to express, purify, and trimerize two RX protein constructs (D1 and D123mut) to study their binding affinity to α-synuclein monomers and oligomers using various methods, including ELISAs. The findings indicated that while D123 showed some binding affinity to α-synuclein oligomers in indirect ELISA, D1 and D123mut did not exhibit sufficient purity or binding capability. The study concluded that optimization of protein constructs and methods is crucial for further exploring RX as a therapeutic protein against Parkinson's disease.
Creative Biolabs provided key reagents and products crucial for this study. Specifically, the Anti-Human SNCA Therapeutic Antibody (Syn-O2, CAT#: TAB-0748CLV) was used as a positive control in the ELISA assays to validate the binding affinity of RX constructs to α-synuclein. Additionally, Creative Biolabs' product ensured reliable comparisons and facilitated the validation process, thereby playing a significant role in the experimental procedures and overall findings of the study.
More Infomation
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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Intrabody
Single-domain Antibody
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Mouse Antibody
Recombinant Antibody
Blocking Antibody
Rabbit Monoclonal Antibody
Neuroscience Antibody
Fab Fragment Antibody
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| IAB-B031(A) | Recombinant Anti-human SNCA Intrabody [(D-Arg)9] | FC, IF, FuncS | scFv-(D-Arg)9 |
| IAB-B031(G) | Recombinant Anti-human SNCA Intrabody [+36 GFP] | CO-IP, FuncS | scFv-(+36GFP) |
| IAB-B031(T) | Recombinant Anti-human SNCA Intrabody [Tat] | ELISA, IF, Neut, FuncS | scFv-Tat |
Customer Reviews and Q&As
Customer Review
Q&As
-
Ideal for SNCA Research
The Anti-SNCA Recombinant Antibody has been excellent for our SNCA research. Its specificity and sensitivity are top-notch, providing clear and reliable results. This antibody has significantly improved the accuracy of our experiments and saved us a lot of troubleshooting time. -
Reliable in Immunoassays
We incorporated this antibody into our immunoassays, and it has been a great asset. The specificity for SNCA is precise, allowing for accurate detection and analysis. The reliable performance has made it an invaluable tool in our lab. -
Consistent and Effective
This antibody has proven to be consistent and effective in our various assays. The high-quality, reproducible results have significantly improved our experiments, making it a crucial part of our research toolkit. The strong affinity and specificity for SNCA have been particularly beneficial.
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# TAB-0748CLV, RRID: AB_3111791)
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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