Mouse Anti-SNCA Recombinant Antibody (TAB-0750CLV) (CAT#: TAB-0750CLV)
This product is a recombinant mouse antibody that recognizes α-synuclein, with a high affinity for aggregated forms of α-synuclein. The binding affinities of this antibody were determined by a variety of methods including isothermal calorimetry, ELISA, and surface plasmon resonance-based approaches. The IC₅₀ value for fibrils is 0.5 nM while for monomers is 3.6 μM. The antibody was expressed in mammalian cells with chemically defined culture media and was purified by affinity chromatography.
Specific Inquiry
ELISA
Figure 1 Anti-Human SNCA Antibody (TAB-0750CLV) in ELISA
ELISA analysis of TAB-0750CLV was performed by coating with Recombinant human alpha-Synuclein Protein (12093-HNAE). Then blocked with BSA and incubated with Anti-Human SNCA Antibody (Syn-O4) (TAB-0750CLV) at a starting concentration of 1 ng/mL. The HRP-conjugated goat anti-mouse IgG as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
WB
Figure 2 Anti-Human SNCA Antibody (TAB-0750CLV) in WB
Western blot analysis was performed by loading Recombinant human alpha-Synuclein Protein (12093-HNAE) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least 1 hour. Membranes were probed with Anti-Human SNCA Antibody (Syn-O4) (TAB-0750CLV) at a dilution of 1:1000 overnight at 4°C on a rocking platform. Then probed with Goat anti-mouse IgG HRP secondary antibody at a dilution of 1:8,000 for one hour. Chemiluminescent was detected.
Lane 1: Non-Reducing Antigen (2.5ug)
Lane 2: Reducing Antigen (2.5ug)
SDS-PAGE
Figure 3 Anti-Human SNCA Antibody (TAB-0750CLV) in SDS-PAGE
SDS-PAGE analysis of TAB-0750CLV in non-reduced (Lane 1) and reduced (Lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.
HPLC
Figure 4 Anti-Human SNCA Antibody (TAB-0750CLV) in HPLC
The purity of TAB-0750CLV was greater than 95% as determined by HPLC.
Specifications
- Immunogen
- α-synuclein oligomers
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human SNCA
- Species Reactivity
- Human
- Applications
- ELISA
- Related Disease
- Parkinson's disease, dementia with Lewy bodies, multiple system atrophy
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- SNCA; synuclein, alpha; PD1; NACP; PARK1; PARK4; Alpha-synuclei
- Gene ID
- 6622
- UniProt ID
- P37840
Related Resources
Sela, Mor, et al. "Brain-Targeted Liposomes Loaded with Monoclonal Antibodies Reduce Alpha-Synuclein Aggregation and Improve Behavioral Symptoms in Parkinson's Disease." Advanced Materials 35.51 (2023): 2304654. https://doi.org/10.1002/adma.202304654
This study explores the development and effectiveness of brain-targeted liposomes (BTL) loaded with monoclonal antibodies (mAbs) to reduce alpha-synuclein aggregation and improve behavioral symptoms in Parkinson's disease (PD). The researchers engineered liposomes conjugated with transferrin to enhance their delivery across the blood-brain barrier (BBB). These liposomes were loaded with the SynO4 antibody, which inhibits alpha-synuclein aggregation, a key pathological feature of PD. The study demonstrated that BTL effectively crossed the BBB, were taken up by neurons, and significantly reduced alpha-synuclein aggregation and neuroinflammation in mouse models of PD. Additionally, BTL treatment improved motor function and learning abilities in these models, showcasing the potential of this targeted delivery system for treating neurodegenerative diseases.
Creative Biolabs contributed significantly to this research by providing the Anti-Human SNCA Therapeutic (SynO4) Antibody (Cat# TAB-0750CLV-L). This antibody was crucial for the experimental evaluation of the BTL's ability to reduce alpha-synuclein aggregation within neurons. By enabling the precise targeting and measurement of alpha-synuclein, the antibody from Creative Biolabs supported the validation of the therapeutic potential of BTL in mitigating the progression of Parkinson's disease.
More Infomation
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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Intrabody Products
Single Domain Antibody Products
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Mouse Antibody Products
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Neuroscience Antibody Products
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Antibody Magnetic Beads
ScFv-Fc Chimera Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| IAB-B031(A) | Recombinant Anti-human SNCA Intrabody [(D-Arg)9] | FC, IF, FuncS | scFv-(D-Arg)9 |
| IAB-B031(G) | Recombinant Anti-human SNCA Intrabody [+36 GFP] | CO-IP, FuncS | scFv-(+36GFP) |
| IAB-B031(T) | Recombinant Anti-human SNCA Intrabody [Tat] | ELISA, IF, Neut, FuncS | scFv-Tat |
Customer Reviews and Q&As
Customer Review
Q&As
James White
Excellent for SNCA Studies
Excellent for SNCA Studies
The Anti-SNCA Recombinant Antibody has been excellent for our alpha-synuclein studies. Its specificity and sensitivity are top-notch, providing clear and reliable results. This antibody has significantly improved the accuracy of our experiments and saved us a lot of troubleshooting time.
05-Nov-23
Laura Harris
Effective in Immunoassays
Effective in Immunoassays
We incorporated this antibody into our immunoassays, and it has been a great asset. The specificity for SNCA is precise, allowing for accurate detection and analysis. The reliable performance has made it an invaluable tool in our lab.
30-Aug-22
David Clark
Consistent High-Quality Results
Consistent High-Quality Results
This antibody has proven to be consistent and high-quality in our assays. The reproducible results and strong affinity for SNCA have significantly improved our experiments, making it a crucial part of our research toolkit. The consistent performance has greatly enhanced our data quality.
23-Jul-21
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# TAB-0750CLV, RRID: AB_3111792)
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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