Anti-TFRC immunotoxin 454A12 (IgG)-RTA
CAT#: AGTO-G068R
This immunotoxin was achieved by conjugating/fusing the Anti-TFRC monoclonal antibody to ricin toxin. It was expressed in CHO and purified with affinity chromatography. The immunotoxin selectively binds to tumor cells. Then the ricin A chain is incorporated into the cell by receptor-mediated endocytosis, blocking ribosomal protein synthesis and ultimately leading to cell death. It was designed for treatment of leptomeningeal carcinomatosis.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to endosomes & lysosomes.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/28598/546_F8_1_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/153/207_B2_1_blue_red_green.jpg
Subcellular Location
Figure 3 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/153/208_B2_1_blue_red_green.jpg
Normal Tissue
Figure 4 Cerebral cortex
Endothelial cells
Staining:
Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/153/2800_B_9_5.jpg
Normal Tissue
Figure 5 Colon
Glandular cells
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/153/2800_A_8_3.jpg
Normal Tissue
Figure 6 Testis
Leydig cells
Staining:
Medium
Intensity: Strong
Quantity: <25%
Pachytene spermatocytes
Staining:
Medium
Intensity: Moderate
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/153/2800_A_6_6.jpg
Normal Tissue
Figure 7 Bone marrow
Hematopoietic cells
Staining:
High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/153/2800_B_6_4.jpg
Normal Tissue
Figure 8 Lymph node
Germinal center cells
Staining:
Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/153/2800_A_8_8.jpg
RNA Expression
Figure 9 RNA cell line category: Cell line enhanced (HDLM-2)
Cell lines ordered by descending RNA expression order.
RNA Expression
Figure 9 RNA cell line category: Cell line enhanced (HDLM-2)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000072274-TFRC
❮
❯
Specifications
- Host Species
- Mouse
- Species Reactivity
- Human
- Clone
- 454A12
- Molecule Class
- IgG-RTA
- Toxin Description
- Ricin consist of two polypeptide chains, A and B, linked by means of disulfide bridge(s). Cytotoxicity is associated with the A chain and its 15 inhibition of protein synthesis in nucleated cells. The B chain is essentially a delivery vehicle for the A chain. The B chain recognizes polysaccharide units at the surface of cells and creates a high affinity interaction with such units. Once the B chain binds with polysaccharide units at 20" the celL surface, the A chain is incorporated into the cell, blocking ribosomal protein synthesis and ultimately leading tocell death. The use of ricin A chain is preferred in this invention.
- Applications
- This product is suitable for use in cytotoxicity assay, functional assay and immunotherapy.
- Related Disease
- Leptomeningeal carcinomatosis
Target
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| TAB-612CT-F(E) | Humanized Anti-TFRC Recombinant Antibody; Fab Fragment (TAB-612CT-F(E)) | ELISA | Humanized Fab |
| TAB-613CT-F(E) | Humanized Anti-TFRC Recombinant Antibody; Fab Fragment (TAB-613CT-F(E)) | ELISA | Humanized Fab |
| TAB-614CT-F(E) | Humanized Anti-TFRC Recombinant Antibody; Fab Fragment (TAB-614CT-F(E)) | ELISA | Humanized Fab |
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| TAB-619CT | Human Anti-TFRC Recombinant Antibody (TAB-619CT) | ELISA, WB | Human antibody |
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0281-YC-F(E) | Mouse Anti-TFRC Recombinant Antibody (clone OX26); Fab Fragment | ELISA | Mouse Fab |
| HPAB-597-FY-F(E) | Human Anti-TFRC Recombinant Antibody; Fab Fragment (HPAB-597-FY-F(E)) | ELISA | Humanized Fab |
| HPAB-0238-YJ-F(E) | Rat Anti-TFRC Recombinant Antibody; Fab Fragment (HPAB-0238-YJ-F(E)) | IHC, FC, WB, FuncS | Rat Fab |
| HPAB-0239-YJ-F(E) | Mouse Anti-TFRC Recombinant Antibody; Fab Fragment (HPAB-0239-YJ-F(E)) | IHC, FC, WB, FuncS | Mouse Fab |
| HPAB-0240-YJ-F(E) | Mouse Anti-TFRC Recombinant Antibody; Fab Fragment (HPAB-0240-YJ-F(E)) | IHC, FC, WB, FuncS | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0238-YJ-S(P) | Rat Anti-TFRC Recombinant Antibody; scFv Fragment (HPAB-0238-YJ-S(P)) | IHC, FC, WB, FuncS | Rat scFv |
| HPAB-0239-YJ-S(P) | Rat Anti-TFRC Recombinant Antibody; scFv Fragment (HPAB-0239-YJ-S(P)) | IHC, FC, WB, FuncS | Rat scFv |
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| HPAB-2692LY-S(P) | Mouse Anti-TFRC Recombinant Antibody; scFv Fragment (HPAB-2692LY-S(P)) | ELISA | Mouse scFv |
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