Anti-TFRC immunotoxin 454A12 (IgG)-RTA (CAT#: AGTO-G068R)
This immunotoxin was achieved by conjugating/fusing the Anti-TFRC monoclonal antibody to ricin toxin. It was expressed in CHO and purified with affinity chromatography. The immunotoxin selectively binds to tumor cells. Then the ricin A chain is incorporated into the cell by receptor-mediated endocytosis, blocking ribosomal protein synthesis and ultimately leading to cell death. It was designed for treatment of leptomeningeal carcinomatosis.
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(Immunofluorescent staining of human cell line U-251 MG shows localization to endosomes & lysosomes.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/28598/546_F8_1_selected.jpg

(Immunofluorescent staining of human cell line A-431 shows localization to vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/153/207_B2_1_blue_red_green.jpg

(Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/153/208_B2_1_blue_red_green.jpg

(Endothelial cells
Staining:
Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/
membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/153/2800_B_9_5.jpg

(Glandular cells
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/153/2800_A_8_3.jpg

(Leydig cells
Staining:
Medium
Intensity: Strong
Quantity: <25%
Pachytene spermatocytes
Staining:
Medium
Intensity: Moderate
Quantity: 75%-25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/153/2800_A_6_6.jpg

(Hematopoietic cells
Staining:
High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/
membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/153/2800_B_6_4.jpg

(Germinal center cells
Staining:
Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/
membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/153/2800_A_8_8.jpg

(Cell lines ordered by descending RNA expression order.)
* https://v21.proteinatlas.org/ENSG00000072274-TFRC

(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000072274-TFRC
Specifications
- Host Species
- Mouse
- Species Reactivity
- Human
- Clone
- 454A12
- Molecule Class
- IgG-RTA
- Toxin Description
- Ricin consist of two polypeptide chains, A and B, linked by means of disulfide bridge(s). Cytotoxicity is associated with the A chain and its 15 inhibition of protein synthesis in nucleated cells. The B chain is essentially a delivery vehicle for the A chain. The B chain recognizes polysaccharide units at the surface of cells and creates a high affinity interaction with such units. Once the B chain binds with polysaccharide units at 20" the celL surface, the A chain is incorporated into the cell, blocking ribosomal protein synthesis and ultimately leading tocell death. The use of ricin A chain is preferred in this invention.
- Applications
- This product is suitable for use in cytotoxicity assay, functional assay and immunotherapy.
- Related Disease
- Leptomeningeal carcinomatosis
Target
- Alternative Names
- TFRC; transferrin receptor; T9; TR; TFR; p90; CD71; TFR1; TRFR; transferrin receptor protein 1; transferrin receptor (p90, CD71); Deglycosylated ricin A chain; Ricin A chain; Ricin A; Ricin; RTA; RT; RA; dgRTA; dgRA; dgA
- Gene ID
- 7037
- UniProt ID
- P02786
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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See other products for "Clone 454A12"
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CAT | Product Name | Application | Type |
---|---|---|---|
MOB-1176z | Mouse Anti-TFRC Recombinant Antibody (MOB-1176z) | ELISA, WB, IP, IF, IHC, FC | Mouse IgG1, κ |
HPAB-2579LY | Mouse Anti-TFRC Recombinant Antibody (HPAB-2579LY) | ELISA | Mouse IgG |
FAMAB-1266CQ | Human Anti-TFRC Recombinant Antibody (clone Q1/71) | WB, FC, IHC | Human IgG1 |
FAMAB-1267CQ | Human Anti-TFRC Recombinant Antibody (FAMAB-1267CQ) | FC | Human IgG1 |
VS-0723-WK21 | Human Anti-TFRC Recombinant Antibody (clone Pabinafusp Alfa) | FC | Human IgG1 |
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