Recombinant Human Anti-TSHR Antibody (M22) (CAT#: PABW-172)

Figure 1 TSHR-stimulating, but not blocking, mAbs to the TSHR induce cAMP production in human thymocytes.

Intracellular cAMP was measured by ELISA after 1-h incubation with the indicated amounts of the mAb with stimulating activity M22 (dotted line), the monoclonal thyroid-blocking Ab K1-70 (solid line), or a normal mouse IgG (dashed line) in human thymocytes. Bars represent mean ± SEM of three to four experiments. All experiments are performed in triplicates; *p < 0.05.

Giménez-Barcons, M., Colobran, R., Gómez-Pau, A., Marín-Sánchez, A., Casteras, A., Obiols, G., ... & Pujol-Borrell, R. (2015). Graves’ disease TSHR-stimulating antibodies (TSAbs) induce the activation of immature thymocytes: a clue to the riddle of TSAbs generation?. The Journal of Immunology, 194(9), 4199-4206.

Figure 2 Kinetics of ¹²⁵I-labeled TSH, M22 IgG, and M22 Fab interacting with TSHR coated tubes.

(A)Time course of binding of ¹²⁵I-labeled TSH, M22 IgG, and M22 Fab (nonspecific binding, i.e., binding of labeled TSH, M22IgG, or M22 Fab to tubes not coated with the TSHR was low (2–2.6% of total cpm added) and was not subtracted from thedata shown in the figure).

Nakatake, N., Sanders, J., Richards, T., Burne, P., Barrett, C., Pra, C. D., ... & Smith, B. R. (2006). Estimation of serum TSH receptor autoantibody concentration and affinity. Thyroid, 16(11), 1077-1084.

Figure 1 Recombinant Human Anti-TSHR Antibody (PABW-172) in SDS-PAGE

SDS-PAGE analysis of PABW-172 in non-reduced (Lane 1) and reduced (Lane 2) conditions.

Figure 2 Recombinant Human Anti-TSHR Antibody (PABW-172) in HPLC

HPLC analysis of PABW-172 shows the purity is>95%.

Figure 3 Recombinant Human Anti-TSHR Antibody (PABW-172) in Dot Blot

Dot Blot analysis of PABW-172 was performed by coating with human TSHR antigen protein.
PABW-172 incubation concentration: 2 ng/μL.
The secondary antibody: HRP-Anti-Human IgG (H+L)

Figure 4 Recombinant Human Anti-TSHR Antibody (PABW-172) in ELISA

ELISA analysis of PABW-172 was performed by coating with recombinant human TSHR antigen. Then blocked with BSA, and incubated with Recombinant Human Anti-TSHR Antibody (M22) (PABW-172). The HRP-conjugated goat anti-human IgG as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.