What does the cKit antibody target?

The antibody targets cKit (CD117), which is the human homolog of the proto-oncogene c-kit. This protein is a type 3 transmembrane receptor for mast cell growth factor (stem cell factor). Mutations in this gene are associated with gastrointestinal stromal tumors.

How does the maitansine payload exert its cytotoxic effect?

Maitansine (or maytansine) inhibits the assembly of microtubules. It binds to tubulin at the rhizoxin binding site, preventing polymerization. This disrupts the formation of the mitotic spindle and causes cell cycle arrest.

Anti-cKit-maitansine ADC (LOP628)

CAT#: ADC-L039

This ADC product is composed of an anti-cKit antibody conjuagated to maitansine (anti-cKit antibody-maitansine). It has demonstrated a response in Inflammatory Diseases treatment by a MOA (Mechanism of Action) of .

Gene Expression

Subcellular Location Figure 1 IF staining of human cell line HEK 293

Immunofluorescent staining of human cell line HEK 293 shows localization to plasma membrane.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/73252/1469_D7_6_selected.jpg

Normal Tissue Figure 2 High expression in breast

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4471/ihc_selected.jpg

Normal Tissue Figure 3 High expression in lung.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3288/8181_A_1_4_val_selected.jpg

Normal Tissue Figure 4 Skin

Melanocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3288/8181_B_8_1.jpg

RNA Expression Figure 5 RNA cell line category: Group enriched (HEL, HMC-1)

Cell lines ordered by descending RNA expression order

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000157404-KIT

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Specifications

Antibody Overview
Humanized IgG1/κ antibody

Antibody Conjugation
Humanized

Linker
non-cleavable linker

Drug
maitansine

Drug Class/Description
Maitansine (INN), or maytansine (USAN), is a cytotoxic agent. It inhibits the assembly of microtubules by binding to tubulin at the rhizoxin binding site.

Target

Introduction
This gene encodes the human homolog of the proto-oncogene c-kit. C-kit was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. This protein is a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). Mutations in this gene are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous lukemia, and piebaldism. Multiple transcript variants encoding different isoforms have been found for this gene.

Alternative Names
PBT; SCFR; C-Kit; CD117

Gene ID
3815

UniProt ID
P10721

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Breast cancer biomarkers at key points during disease progression

Precise cKit Receptor Targeting

Targeting CD117 with this humanized antibody was straightforward and yielded high-quality binding data. The antibody recognized the receptor on the cell surface effectively. We are studying the effects of maitansine on microtubule assembly, and this product has been essential.

Potent Microtubule Assembly Inhibition

The maitansine payload is a very effective cytotoxic agent in our models. We observed clear inhibition of microtubule assembly at the rhizoxin binding site. The non-cleavable linker ensured that the payload remained attached until internalization, reducing background noise in our assays.

Q&As

What does the cKit antibody target?

A: The antibody targets cKit (CD117), which is the human homolog of the proto-oncogene c-kit. This protein is a type 3 transmembrane receptor for mast cell growth factor (stem cell factor). Mutations in this gene are associated with gastrointestinal stromal tumors.
How does the maitansine payload exert its cytotoxic effect?

A: Maitansine (or maytansine) inhibits the assembly of microtubules. It binds to tubulin at the rhizoxin binding site, preventing polymerization. This disrupts the formation of the mitotic spindle and causes cell cycle arrest.

View the frequently asked questions answered by Creative Biolabs Support.

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Downloadable Resources

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Datasheet

Datasheet - ADC-L039.pdf

MSDS

MSDS - ADC-L039.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

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