Knock-Valid™ Rabbit Anti-p53 (Ab-15) Antibody

Name: Knock-Valid™ Rabbit Anti-p53 (Ab-15) Antibody
SKU: VS-1225-XU124
Rating: 5 (1 reviews)

CAT#: VS-1225-XU124

This versatile antibody is optimized for WB, IHC, IF, and ELISA, making it a comprehensive tool for p53 research. As a Knock-Valid™ product, its performance is strictly validated using p53-knockout cell lines, ensuring that signals in IHC represent authentic p53 accumulation. It is ideal for researchers monitoring the early stages of p53 activation and stabilization in cancer biology, as well as for screening p53-stabilizing compounds in high-throughput ELISA formats.

Gene Expression

Subcellular Location Figure 1 IF staining of human cell line A-431

Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & vesicles.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/39239/651_G2_2_selected.jpg

Subcellular Location Figure 2 IF staining of human cell line A549

Immunofluorescent staining of human cell line A549 shows localization to nucleoplasm.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/63532/1113_C11_2_selected.jpg

Subcellular Location Figure 3 IF staining of human cell line U-2 OS

Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm, cytosol & mitochondria.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/79965/1999_E7_4_selected.jpg

Subcellular Location Figure 4 IF staining of human cell line U-251 MG

Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & vesicles.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/39238/656_H2_1_red_green.jpg

Normal Tissue Figure 5 Oral mucosa

Squamous epithelial cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Nuclear

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2973/6986_A_8_1.jpg

Normal Tissue Figure 6 Colon

Enterocytes
Staining: Medium
Intensity: Strong
Quantity: <25%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2973/6986_A_9_3.jpg

RNA Expression Figure 7 RNA cell line category: Low cell line specificity

Cell lines ordered by descending RNA expression order.

RNA Expression Figure 7 RNA cell line category: Low cell line specificity

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000141510-TP53

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Specifications

Host Species
Rabbit

Specificity
p53 (Ab-15)

Species Reactivity
Human; Mouse; Rat; Monkey

Applications
WB; IHC; IF; ELISA

Conjugate
Unconjugated

Product Property

Clonality
Polyclonal

Purification
Antibodies were generated through rabbit immunization using KLH-coupled synthetic phosphopeptides. Purification was achieved via affinity chromatography with phosphopeptide epitopes, followed by negative selection employing corresponding non-phosphorylated peptides to eliminate cross-reactive antibodies.

Concentration
1.0 mg/ml

Storage
Store at -20˚C

Shipping
Ice Packs

Applications

Application Notes
WB: 1:500-1:2000
IHC: 1:100-1:300
IF: 1:200-1:1000

Target

Alternative Names
BCC7; BMFS5; LFS1; TP53; TRP53

Gene ID
7157

UniProt ID
P04637

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Related Diseases

EGFR Tyrosine Kinase Inhibitor Resistance

Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - VS-1225-XU124.pdf

MSDS

MSDS - VS-1225-XU124.pdf

COA
Certificate of Analysis Lookup

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