anti-HLA-A immunotoxin Fib75 (IgG)-α-sar

This immunotoxin was achieved by conjugating/fusing the anti-HLA-A monoclonal antibody to α-sarcin. It was expressed in CHO and purified with affinity chromatography. The immunotoxin selectively binds to human bladder carcinoma cells. Once bind, alpha-Sarcin enters into the cells by receptor-mediated endocytosis and interacts with a single bond in the targeted ribosome and breaks it, causing the ribosome to be inactivated. It was designed for treatment of bladder carcinoma.

Specifications

Host Species
Mouse

Species Reactivity
Human

Clone
Fib75

Molecule Class
L kappa; IgG2a-α-sar

Toxin Description
Alpha-Sarcin is a ribosome-inactivating protein produced in the mold Aspergillus giganteus. Specifically, alpha-sarcin cleaves the a portion of ribosomal RNA that forms the small ribosomal substrate. The high specificity of alpha-sarcin and its efficiency of cleavage are point of study and also account for this protein's very high toxicity level.

Applications
This product is suitable for use in cytotoxicity assay, functional assay and immunotherapy.

Related Disease
Bladder cancer

Target

Alternative Names
antigen presenting molecule; FLJ26655; HLA class I histocompatibility antigen, A-1 alpha chain; HLAA; leukocyte antigen class I-A; major histocompatibility complex, class I, A; MHC class I antigen HLA-A heavy chain; HLA class I-related plasma membrane antigen; Bladder cance; HLA class I-related plasma membrane antigen; Bladder cance α-sar; α-sarcin

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

See other products for "HLA-A"

3D IHC3D Immunohistochemistry

ActivActivation

AgonistAgonist

ApopApoptosis

BABioassay

BIBioimaging

BlockBlocking

Cell ScreeningCell Screening

SeparationCell Separation

ChIPChromatin Immunoprecipitation

CMCDComplement Mediated Cell Depletion

CostimCostimulation

CytCytotoxicity

DepletionDepletion

DBDot Blot

EMElectron Microscopy

ELISAEnzyme-linked Immunosorbent Assay

ELISPOTEnzyme-linked Immunosorbent Spot

FCFlow Cytometry

FuncSFunctional Assay

GSGel Super Shift Assay

HAHemagglutination

IAImmunoassay

IBImmunoblotting

ICCImmunocytochemistry

IDImmunodiffusion

IFImmunofluorescence

IHCImmunohistochemistry

IHC-FrImmunohistochemistry-Frozen

IHC-PImmunohistochemistry-Paraffin

REImmunohistology - Resin Sections

IPImmunoprecipitation

IRMAImmunoradiometric Assay

SHIn situ hybridization

InhibInhibition

ICFCIntracellular Staining for Flow Cytometry

KO/KD-WBKnockout/Knockdown target confirmation by Western Blot

Live cell imagingLive cell imaging

CyTOF®Mass Cytometry

MeDIPMethylated DNA Immunoprecipitation

MultiplexMultiplex bead-based assay

NeutNeutralization

PPProtein Purification

PGProteogenomics

RIRadial Immunodiffusion

RIARadioimmunoassay

StimStimulation

SPRSurface Plasmon Resonance

TCTissue Culture

TBTurbidimetry

WBWestern Blot

anti-HLA-A immunotoxin Fib75 (IgG)-α-sar