Recombinant Human Antibody (HYB3) is capable of binding to HLA-A1. MAGE-A1, expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-HLA-A1. MAGE-A1 mAb and CH1-3 region of human IgG1 and a light chain (LC) encoding VL from anti-HLA-A1 mAb and CL of human kappa light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition. It recognizes the C-terminal half of the MAGE-A1 peptide, the HLA-A1 alpha1-helix, and N-terminal residues of the alpha2-helix, accompanied by a large tilting angle between the two types of molecules within the complex.
Figure 1 Fine specificity of the affinity-matured clones by phage-ELISA.
pMHC complexes were refolded using each peptide and coated via streptavidin on an ELISA plate. Equal concentrations of phage-Abs were incubated for 1 h at room temperature. After extensive washing, bound phages were detected with an anti-M13 mAb coupled to HRP.
Chames, P., Willemsen, R. A., Rojas, G., Dieckmann, D., Rem, L., Schuler, G., ... & Hoogenboom, H. R. (2002). TCR-like human antibodies expressed on human CTLs mediate antibody affinity-dependent cytolytic activity. The Journal of Immunology, 169(2), 1110-1118.
Figure 2 scFv G8 and Hyb3 specifically recognize M1/A1 on cells.
Flow cytometry of HLA-A1+ B cells (APD) with or without MAGE A1 peptide with Hyb3. Histograms showing binding of scFv G8 and Hyb3 at a concentration of 10 µg/mL to APD cells pulsed with peptide MAGE A1. Phage Hyb3 (purple) were used as a positive control for binding to APD cells.
Saeed, M., van Brakel, M., Zalba, S., Schooten, E., Rens, J. A., Koning, G. A., ... & ten Hagen, T. L. (2016). Targeting melanoma with immunoliposomes coupled to anti-MAGE A1 TCR-like single-chain antibody. International journal of nanomedicine, 11, 955.
Figure 3 Binding of immunoliposomes to APD cells.
Confocal images of APD cells (500,000 cells per mL) pulsed with peptide and incubated with immunoliposomes for 2 hours at 37°C.
Saeed, M., van Brakel, M., Zalba, S., Schooten, E., Rens, J. A., Koning, G. A., ... & ten Hagen, T. L. (2016). Targeting melanoma with immunoliposomes coupled to anti-MAGE A1 TCR-like single-chain antibody. International journal of nanomedicine, 11, 955.
Figure 4 Purification of scFv Hyb3 proteins by IMAC.
Western blot analyses of purity and size of purified scFv proteins. Fractions 7 and 8 consist of the peak fractions from the chromatogram and were run on SDS-PAGE and subjected by Western blot using anti-HIS mAb HRP.
Saeed, M., van Brakel, M., Zalba, S., Schooten, E., Rens, J. A., Koning, G. A., ... & ten Hagen, T. L. (2016). Targeting melanoma with immunoliposomes coupled to anti-MAGE A1 TCR-like single-chain antibody. International journal of nanomedicine, 11, 955.
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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CAT | Product Name | Application | Type |
---|---|---|---|
MOB-500 | Recombinant Anti-human HLA-A Antibody | WB, FC, FuncS | IgG |
MOB-2307MZ | Recombinant Mouse Anti-Human HLA-A Antibody (clone 9.M.208), Biotin-Conjugated | FC, ICC/IF | Mouse antibody |
HPAB-0349CQ | Mouse Anti-HLA-A Recombinant Antibody (clone 2D7sc(Fv)2) | WB, FC | Mouse sc(Fv)2 |
HPAB-1096WJ | Mouse Anti-HLA-A Recombinant Antibody (HPAB-1096WJ) | ELISA | Mouse IgG1 |
VS3-FY676 | Recombinant Rabbit Anti-HLA-A Antibody (clone R05-5H7) | WB, IHC-Fr, IHC-P, ICC, IF, IP | Rabbit IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
MOB-500-F(E) | Recombinant Anti-human HLA-A Antibody Fab Fragment | Neut, IF, FuncS | Fab |
MHH-500-F(E) | Recombinant Human Anti-human HLA-A Antibody Fab Fragment | WB, IF, FuncS | Fab |
PFBC-072 | Human Anti-HLA-A Recombinant Antibody (clone HYB3); Fab Fragment | WB, ELISA | Human Fab |
HPAB-1096WJ-F(E) | Mouse Anti-HLA-A Recombinant Antibody; Fab Fragment (HPAB-1096WJ-F(E)) | ELISA | Mouse Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
MOB-500-S(P) | Recombinant Anti-human HLA-A Antibody scFv Fragment | ELISA, WB, IF, FuncS | scFv |
MHH-500-S(P) | Recombinant Human Anti-human HLA-A Antibody scFv Fragment | ELISA, WB, FuncS | scFv |
PSBC-072 | Human Anti-HLA-A Recombinant Antibody (clone HYB3); scFv Fragment | WB, ELISA | Human scFv |
HPAB-1096WJ-S(P) | Mouse Anti-HLA-A Recombinant Antibody; scFv Fragment (HPAB-1096WJ-S(P)) | ELISA | Mouse scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
AGTO-L022F | anti-HLA-A immunotoxin Fib75 (IgG)-α-sar | Cytotoxicity assay, Functional assay |
CAT | Product Name | Application | Type |
---|---|---|---|
PABX-105 | Recombinant Human Anti-HLA-A Antibody (T1) | WB, ELISA, FuncS | IgG |
PABX-105-S (P) | Recombinant Human Anti-HLA-A Antibody scFv Fragment (T1) | WB, ELISA, FuncS | scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
MOR-1617 | Rabbit Anti-HLA-A Recombinant Antibody (clone DS1617AB) | FC, ICC, IF, IHC-Fr, IHC-P, IP, WB | Rabbit IgG |
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