Human Anti-HLA-A Recombinant Antibody (clone HYB3) (CAT#: PABC-072)
Recombinant Human Antibody (HYB3) is capable of binding to HLA-A1. MAGE-A1, expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-HLA-A1. MAGE-A1 mAb and CH1-3 region of human IgG1 and a light chain (LC) encoding VL from anti-HLA-A1 mAb and CL of human kappa light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition. It recognizes the C-terminal half of the MAGE-A1 peptide, the HLA-A1 alpha1-helix, and N-terminal residues of the alpha2-helix, accompanied by a large tilting angle between the two types of molecules within the complex.
Specific Inquiry
ELISA
Figure 1 Fine specificity of the affinity-matured clones by phage-ELISA.
pMHC complexes were refolded using each peptide and coated via streptavidin on an ELISA plate. Equal concentrations of phage-Abs were incubated for 1 h at room temperature. After extensive washing, bound phages were detected with an anti-M13 mAb coupled to HRP.
Chames, P., Willemsen, R. A., Rojas, G., Dieckmann, D., Rem, L., Schuler, G., ... & Hoogenboom, H. R. (2002). TCR-like human antibodies expressed on human CTLs mediate antibody affinity-dependent cytolytic activity. The Journal of Immunology, 169(2), 1110-1118.
FC
Figure 2 scFv G8 and Hyb3 specifically recognize M1/A1 on cells.
Flow cytometry of HLA-A1+ B cells (APD) with or without MAGE A1 peptide with Hyb3. Histograms showing binding of scFv G8 and Hyb3 at a concentration of 10 µg/mL to APD cells pulsed with peptide MAGE A1. Phage Hyb3 (purple) were used as a positive control for binding to APD cells.
Saeed, M., van Brakel, M., Zalba, S., Schooten, E., Rens, J. A., Koning, G. A., ... & ten Hagen, T. L. (2016). Targeting melanoma with immunoliposomes coupled to anti-MAGE A1 TCR-like single-chain antibody. International journal of nanomedicine, 11, 955.
IF
Figure 3 Binding of immunoliposomes to APD cells.
Confocal images of APD cells (500,000 cells per mL) pulsed with peptide and incubated with immunoliposomes for 2 hours at 37°C.
Saeed, M., van Brakel, M., Zalba, S., Schooten, E., Rens, J. A., Koning, G. A., ... & ten Hagen, T. L. (2016). Targeting melanoma with immunoliposomes coupled to anti-MAGE A1 TCR-like single-chain antibody. International journal of nanomedicine, 11, 955.
WB
Figure 4 Purification of scFv Hyb3 proteins by IMAC.
Western blot analyses of purity and size of purified scFv proteins. Fractions 7 and 8 consist of the peak fractions from the chromatogram and were run on SDS-PAGE and subjected by Western blot using anti-HIS mAb HRP.
Saeed, M., van Brakel, M., Zalba, S., Schooten, E., Rens, J. A., Koning, G. A., ... & ten Hagen, T. L. (2016). Targeting melanoma with immunoliposomes coupled to anti-MAGE A1 TCR-like single-chain antibody. International journal of nanomedicine, 11, 955.
Specifications
- Immunogen
- Human major histocompatibility complex, class I, A
- Host Species
- Human
- Type
- Human IgG
- Specificity
- Human HLA-A
- Species Reactivity
- Human
- Clone
- HYB3
- Applications
- WB, ELISA, IF, FC
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been reported for use in Western Blot, Enzyme-Linked Immunosorbent Assay, Immunofluorescence and Flow Cytometry.
Target
- Alternative Names
- HLA-A; major histocompatibility complex, class I, A; HLAA; HLA class I histocompatibility antigen, A-1 alpha chain; antigen presenting molecule; leukocyte antigen class I-A; MHC class I antigen HLA-A heavy chain
- Gene ID
- 3105
- UniProt ID
- P30443
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-500 | Recombinant Anti-human HLA-A Antibody | WB, FC, FuncS | IgG |
| MOB-2307MZ | Recombinant Mouse Anti-Human HLA-A Antibody (clone 9.M.208), Biotin-Conjugated | FC, ICC/IF | Mouse antibody |
| HPAB-0349CQ | Mouse Anti-HLA-A Recombinant Antibody (clone 2D7sc(Fv)2) | WB, FC | Mouse sc(Fv)2 |
| HPAB-1096WJ | Mouse Anti-HLA-A Recombinant Antibody (HPAB-1096WJ) | ELISA | Mouse IgG1 |
| VS3-FY676 | Recombinant Rabbit Anti-HLA-A Antibody (clone R05-5H7) | WB, IHC-Fr, IHC-P, ICC, IF, IP | Rabbit IgG |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-500-F(E) | Recombinant Anti-human HLA-A Antibody Fab Fragment | Neut, IF, FuncS | Fab |
| MHH-500-F(E) | Recombinant Human Anti-human HLA-A Antibody Fab Fragment | WB, IF, FuncS | Fab |
| PFBC-072 | Human Anti-HLA-A Recombinant Antibody (clone HYB3); Fab Fragment | WB, ELISA | Human Fab |
| HPAB-1096WJ-F(E) | Mouse Anti-HLA-A Recombinant Antibody; Fab Fragment (HPAB-1096WJ-F(E)) | ELISA | Mouse Fab |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-500-S(P) | Recombinant Anti-human HLA-A Antibody scFv Fragment | ELISA, WB, IF, FuncS | scFv |
| MHH-500-S(P) | Recombinant Human Anti-human HLA-A Antibody scFv Fragment | ELISA, WB, FuncS | scFv |
| PSBC-072 | Human Anti-HLA-A Recombinant Antibody (clone HYB3); scFv Fragment | WB, ELISA | Human scFv |
| HPAB-1096WJ-S(P) | Mouse Anti-HLA-A Recombinant Antibody; scFv Fragment (HPAB-1096WJ-S(P)) | ELISA | Mouse scFv |
Immunotoxin
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AGTO-L022F | anti-HLA-A immunotoxin Fib75 (IgG)-α-sar | Cytotoxicity assay, Functional assay |
Human Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABX-105 | Recombinant Human Anti-HLA-A Antibody (T1) | WB, ELISA, FuncS | IgG |
| PABX-105-S (P) | Recombinant Human Anti-HLA-A Antibody scFv Fragment (T1) | WB, ELISA, FuncS | scFv |
MHC Tetramer for Other Applications
MHC Tetramer for Cancer
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1617 | Rabbit Anti-HLA-A Recombinant Antibody (clone DS1617AB) | FC, ICC, IF, IHC-Fr, IHC-P, IP, WB | Rabbit IgG |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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