Rabbit Anti-AKT1 Polyclonal Antibody
CAT#: VS3-WK1562
This antibody is a rabbit polyclonal antibody against human AKT1, which is catalytically inactive in serum-starved primary and immortalized fibroblasts. It was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen (synthesized peptide derived from human AKT1). It can detect endogenous levels of AKT1. This product was tested for use in WB and ELISA.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & microtubules.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2891/39_F7_2_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human gall bladder
Immunohistochemical staining of human gall bladder shows strong nuclear positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2891/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IHC staining of human cerebellum
Immunohistochemical staining of human cerebellum shows strong cytoplasmic and nuclear positivity in Purkinje cells and cells in molecular layer.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3765/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 4 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & microtubules.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2891/38_F7_1_red_green.jpg
Subcellular Location and Protein Expression
Figure 5 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & microtubules.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2891/40_F7_1_red_green.jpg
Normal Tissue
Figure 6 Cerebral cortex
Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Glial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Neuronal cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2891/7680_B_8_5.jpg
Normal Tissue
Figure 7 Colon
Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Glandular cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Peripheral nerve/ganglion
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2891/7680_A_7_3.jpg
Normal Tissue
Figure 8 Liver
Cholangiocytes
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2891/7680_A_8_4.jpg
Normal Tissue
Figure 9 Kidney
Cells in glomeruli
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Cells in tubules
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2891/7680_A_9_5.jpg
Normal Tissue
Figure 10 Testis
Elongated or late spermatids
Staining:High
Intensity: Strong
Quantity:>75%
Leydig cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Pachytene spermatocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Peritubular cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Preleptotene spermatocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Round or early spermatids
Staining:High
Intensity: Strong
Quantity:>75%
Sertoli cells
Staining:High
Intensity: Strong
Quantity:>75%
Spermatogonia cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2891/7680_A_4_6.jpg
Normal Tissue
Figure 11 Lymph node
Germinal center cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Non-germinal center cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2891/7680_A_8_8.jpg
RNA Expression
Figure 12 RNA cell line category: Cell line enhanced (MCF7)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000142208-AKT1
❮
❯
Specifications
- Immunogen
- Synthesized peptide derived from human Akt
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human AKT1
- Species Reactivity
- Human, Mouse, Rat
- Applications
- WB, ELISA
- Conjugate
- Unconjugated
Product Property
- Clonality
- Polyclonal
- Purification
- Affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Aggregation
- < 5%
- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- PBS, 0.5% BSA, 50% Glycerol
- Preservative
- 0.02% Sodium Azide
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- This antibody has been tested for use in Western Blot (1:500) and ELISA (1:10000-1/20000).
Target
- Alternative Names
- AKT Serine/Threonine Kinase 1; V-Akt Murine Thymoma Viral Oncogene Homolog 1; Protein Kinase B Alpha; Proto-Oncogene C-Akt; RAC-PK-Alpha; EC 2.7.11.1; PKB Alpha; PKB; RAC; V-Akt Murine Thymoma Viral Oncogene-Like Protein 1; RAC-Alpha Serine/Threonine-Protein Kinase
- Gene ID
- 207
- UniProt ID
- P31749
- Long Name
- AKT Serine/Threonine Kinase 1
- Sequence Similarities
- Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily.
- Cellular Localization
- Cell membrane, Cytoplasm, Membrane, Nucleus
- Post Translation Modifications
- O-GlcNAcylation at Thr-305 and Thr-312 inhibits activating phosphorylation at Thr-308 via disrupting the interaction between AKT1 and PDPK1. O-GlcNAcylation at Ser-473 also probably interferes with phosphorylation at this site.
Phosphorylation on Thr-308, Ser-473 and Tyr-474 is required for full activity.
- Protein Refseq
- NP_001014431.1
- Function
- AKT1 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates.
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Related Signaling Pathways
BCR Signaling Pathway
ErbB Signaling Pathway
Insulin Signaling Pathway
mTOR Signaling Pathway
PI3K-Akt Signaling Pathway
Ras Signaling Pathway
Related Diseases
Breast Cancer
Colorectal Cancer
Endometrial Cancer
Gastric Cancer
Hepatocellular Carcinoma
Non-small Cell Lung Cancer
Prostate Cancer
Endocrine Resistance
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "AKT1"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| NAB-33-VHH | Recombinant Anti-human AKT1 VHH Single Domain Antibody | WB, IP, ChiP, Neut, ELISA | Llama VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0031MZ | Chicken Anti-AKT1 (ab1) Polyclonal IgY | WB | Chicken antibody |
| BRD-0032MZ | Chicken Anti-AKT1 (ab2) Polyclonal IgY | Indirect ELISA, WB | Chicken antibody |
| BRD-0683MZ | Chicken Anti-AKT1 Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0126 | Hi-Affi™ Rabbit Anti-AKT1 Recombinant Antibody (clone DS126AB) | WB | Rabbit IgG |
| MOR-4668 | Hi-Affi™ Rabbit Anti-AKT1 Recombinant Antibody (clone TH182DS) | WB, IF, ICC, IHC-P, FC, ELISA | Rabbit IgG |
| MOR-4669 | Hi-Affi™ Rabbit Anti-AKT1 Recombinant Antibody (clone TH183DS) | WB, IF, ICC, FC, ELISA | Rabbit IgG |
| MOR-4670 | Hi-Affi™ Rabbit Anti-AKT1 Recombinant Antibody (clone TH184DS) | WB, IF, ICC, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-0043-CN | Rabbit Anti-AKT1 Recombinant Antibody (clone CBACN-016) | WB, IF, IHC, IP, FC | Rabbit IgG |
| MRO-0044-CN | Mouse Anti-AKT1 Recombinant Antibody (clone D9-9-C9) | WB, IF, IHC, FC | Mouse IgG2b |
| MRO-2295-CN | Rabbit Anti-AKT1 Recombinant Antibody (clone CBACN-590) | WB, IF, IHC, IP | Rabbit IgG |
| MRO-2296-CN | Rabbit Anti-AKT1 Recombinant Antibody (clone CBACN-591) | WB, IF, IHC | Rabbit IgG |
| MRO-2297-CN | Rabbit Anti-AKT1 Recombinant Antibody (clone CBACN-592) | WB, IHC, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY10 | AbPlus™ Anti-AKT1 Magnetic Beads (CBACN-016) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY15 | Rabbit Anti-NHP AKT1 Recombinant Antibody (VS-1024-XY15) | WB, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC39 | CytoStream™ Mouse Anti-AKT1 Recombinant Antibody (VS13-YC39) | WB, ICC, IF, IHC-P, FC | Mouse IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY278 | Anti-AKT1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY279 | Anti-Mouse AKT1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY281 | Anti-Zebrafish AKT1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY280 | Anti-Rat AKT1 Immunohistochemistry Kit | IHC |
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