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    Rabbit Anti-AKT1 (pS124) Recombinant Antibody (clone R04-4X5) (CAT#: VS3-WK2156)

    This product is a rabbit recombinant antibody that is specific to phosphorylated AKT1. The target of this antibody is phospho-AKT1 (Ser124). The clone R04-4X5 can be used in WB analysis at a dilution of 1/500-1/1000 and IP analysis at a dilution of 1/20. The molecular weight of AKT1 (pSer124) observed in WB experiments using this antibody is 60 kDa.

    Specific Inquiry
    • Size:

    • Conjugation:

    • Endotoxin:

    • Purity:

    • Formats:

    • Isotype Switching:

    • Fc Engineering:

    • Modalities:

    Custom Production

    We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

    Sequences:
    Isotype :
    Purity :
    Endotoxin :
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    Conjugation :
    Fc Engineering :
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    Other Requirements:
    We require custom production
    • Gene Expression
    • Datasheet
    • MSDS
    • COA
    Subcellular Location and Protein Expression
    Figure 1 IF staining of human cell line A-431
    Figure 1 IF staining of human cell line A-431
    (Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & microtubules.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2891/39_F7_2_selected.jpg
    Figure 2 IHC staining of human gall bladder
    Figure 2 IHC staining of human gall bladder
    (Immunohistochemical staining of human gall bladder shows strong nuclear positivity in glandular cells.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2891/ihc_selected.jpg
    Figure 3 IHC staining of human cerebellum
    Figure 3 IHC staining of human cerebellum
    (Immunohistochemical staining of human cerebellum shows strong cytoplasmic and nuclear positivity in Purkinje cells and cells in molecular layer.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3765/ihc_selected.jpg
    Figure 4 IF staining of human cell line U-2 OS
    Figure 4 IF staining of human cell line U-2 OS
    (Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & microtubules.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2891/38_F7_1_red_green.jpg
    Figure 5 IF staining of human cell line U-251 MG
    Figure 5 IF staining of human cell line U-251 MG
    (Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & microtubules.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2891/40_F7_1_red_green.jpg
    Normal Tissue
    Figure 6 Cerebral cortex
    Figure 6 Cerebral cortex
    (Endothelial cells
    Staining:Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Nuclear
    Glial cells
    Staining:Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Nuclear
    Neuronal cells
    Staining:High
    Intensity: Strong
    Quantity:>75%
    Location: Cytoplasmic/membranous nuclear)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2891/7680_B_8_5.jpg
    Figure 7 Colon
    Figure 7 Colon
    (Endothelial cells
    Staining:Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Nuclear
    Glandular cells
    Staining:High
    Intensity: Strong
    Quantity:>75%
    Location: Nuclear
    Peripheral nerve/ganglion
    Staining:Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Nuclear)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2891/7680_A_7_3.jpg
    Figure 8 Liver
    Figure 8 Liver
    (Cholangiocytes
    Staining:High
    Intensity: Strong
    Quantity:>75%
    Location: Nuclear)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2891/7680_A_8_4.jpg
    Figure 9 Kidney
    Figure 9 Kidney
    (Cells in glomeruli
    Staining:Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Nuclear
    Cells in tubules
    Staining:Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Nuclear)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2891/7680_A_9_5.jpg
    Figure 10 Testis
    Figure 10 Testis
    (Elongated or late spermatids
    Staining:High
    Intensity: Strong
    Quantity:>75%
    Leydig cells
    Staining:Medium
    Intensity: Moderate
    Quantity:>75%
    Pachytene spermatocytes
    Staining:Medium
    Intensity: Moderate
    Quantity:>75%
    Peritubular cells
    Staining:Medium
    Intensity: Moderate
    Quantity: 75%-25%
    Preleptotene spermatocytes
    Staining:Medium
    Intensity: Moderate
    Quantity:>75%
    Round or early spermatids
    Staining:High
    Intensity: Strong
    Quantity:>75%
    Sertoli cells
    Staining:High
    Intensity: Strong
    Quantity:>75%
    Spermatogonia cells
    Staining:Medium
    Intensity: Moderate
    Quantity:>75%)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2891/7680_A_4_6.jpg
    Figure 11 Lymph node
    Figure 11 Lymph node
    (Germinal center cells
    Staining:High
    Intensity: Strong
    Quantity:>75%
    Location: Nuclear
    Non-germinal center cells
    Staining:High
    Intensity: Strong
    Quantity:>75%
    Location: Nuclear)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2891/7680_A_8_8.jpg
    RNA Expression
    Figure 12 RNA cell line category: Cell line enhanced (MCF7)
    Figure 12 RNA cell line category: Cell line enhanced (MCF7)
    (Cell lines ordered by descending RNA expression order.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000142208-AKT1

    Specifications

    • Host Species
    • Rabbit
    • Type
    • Rabbit IgG
    • Specificity
    • Phospho-Ser124 of human, mouse and rat AKT1
    • Species Reactivity
    • Human, Mouse, Rat
    • Clone
    • R04-4X5
    • Applications
    • WB, IP
    • Conjugate
    • Unconjugated

    Product Property

    • Clonality
    • Monoclonal
    • Purification
    • Protein A affinity purified
    • Purity
    • >95% as determined by SDS-PAGE
    • Format
    • Liquid
    • Concentration
    • Please refer to the vial label for the specific concentration.
    • Buffer
    • PBS (pH 7.4), 150 mM NaCl and 50% Glycerol
    • Preservative
    • 0.02% Sodium azide
    • Storage
    • Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
    • Shipping
    • Ice packs

    Applications

    • Application Notes
    • WB: 1/500-1/1000
      IP: 1/20

    Target

    • Alternative Names
    • AKT Serine/Threonine Kinase 1; V-Akt Murine Thymoma Viral Oncogene Homolog 1; Protein Kinase B Alpha; Proto-Oncogene C-Akt; RAC-PK-Alpha; EC 2.7.11.1; PKB Alpha; PKB; RAC; V-Akt Murine Thymoma Viral Oncogene-Like Protein 1; RAC-Alpha Serine/Threonine-Protein Kinase
    • Long Name
    • AKT Serine/Threonine Kinase 1

    Recommended Products

    Isotype Control

    Secondary Antibody

    Related Resources

    • Related Signaling Pathways
    • Related Diseases

    Product Notes

    This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

    • Increased sensitivity
    • Confirmed specificity
    • High repeatability
    • Excellent batch-to-batch consistency
    • Sustainable supply
    • Animal-free production

    See more details about Hi-Affi™ recombinant antibody benefits.

    Downloads

    Download resources about recombinant antibody development and antibody engineering to boost your research.

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    For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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