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    Human Anti-AKT1 Recombinant Antibody (clone 30769) (CAT#: VS-0224-XY5)

    Provided is a bivalent human recombinant anti-AKT1 antibody selected from the HuCAL® phage display library, expressed in E. coli. It was purified by affinity chromatography. This product has been tested using Western blot and other applications.

    Specific Inquiry
    • Size:

    • Conjugation:

    • Endotoxin:

    • Purity:

    • Formats:

    • Isotype Switching:

    • Fc Engineering:

    • Modalities:

    Custom Production

    We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

    Sequences:
    Isotype :
    Purity :
    Endotoxin :
    Quantity :
    Conjugation :
    Fc Engineering :
    Modalities :
    Other Requirements:
    We require custom production
    • Gene Expression
    • Datasheet
    • MSDS
    • COA
    Subcellular Location and Protein Expression
    Figure 1 IF staining of human cell line A-431
    Figure 1 IF staining of human cell line A-431
    (Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & microtubules.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2891/39_F7_2_selected.jpg
    Figure 2 IHC staining of human gall bladder
    Figure 2 IHC staining of human gall bladder
    (Immunohistochemical staining of human gall bladder shows strong nuclear positivity in glandular cells.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2891/ihc_selected.jpg
    Figure 3 IHC staining of human cerebellum
    Figure 3 IHC staining of human cerebellum
    (Immunohistochemical staining of human cerebellum shows strong cytoplasmic and nuclear positivity in Purkinje cells and cells in molecular layer.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3765/ihc_selected.jpg
    Figure 4 IF staining of human cell line U-2 OS
    Figure 4 IF staining of human cell line U-2 OS
    (Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & microtubules.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2891/38_F7_1_red_green.jpg
    Figure 5 IF staining of human cell line U-251 MG
    Figure 5 IF staining of human cell line U-251 MG
    (Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & microtubules.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2891/40_F7_1_red_green.jpg
    Normal Tissue
    Figure 6 Cerebral cortex
    Figure 6 Cerebral cortex
    (Endothelial cells
    Staining:Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Nuclear
    Glial cells
    Staining:Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Nuclear
    Neuronal cells
    Staining:High
    Intensity: Strong
    Quantity:>75%
    Location: Cytoplasmic/membranous nuclear)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2891/7680_B_8_5.jpg
    Figure 7 Colon
    Figure 7 Colon
    (Endothelial cells
    Staining:Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Nuclear
    Glandular cells
    Staining:High
    Intensity: Strong
    Quantity:>75%
    Location: Nuclear
    Peripheral nerve/ganglion
    Staining:Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Nuclear)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2891/7680_A_7_3.jpg
    Figure 8 Liver
    Figure 8 Liver
    (Cholangiocytes
    Staining:High
    Intensity: Strong
    Quantity:>75%
    Location: Nuclear)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2891/7680_A_8_4.jpg
    Figure 9 Kidney
    Figure 9 Kidney
    (Cells in glomeruli
    Staining:Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Nuclear
    Cells in tubules
    Staining:Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Nuclear)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2891/7680_A_9_5.jpg
    Figure 10 Testis
    Figure 10 Testis
    (Elongated or late spermatids
    Staining:High
    Intensity: Strong
    Quantity:>75%
    Leydig cells
    Staining:Medium
    Intensity: Moderate
    Quantity:>75%
    Pachytene spermatocytes
    Staining:Medium
    Intensity: Moderate
    Quantity:>75%
    Peritubular cells
    Staining:Medium
    Intensity: Moderate
    Quantity: 75%-25%
    Preleptotene spermatocytes
    Staining:Medium
    Intensity: Moderate
    Quantity:>75%
    Round or early spermatids
    Staining:High
    Intensity: Strong
    Quantity:>75%
    Sertoli cells
    Staining:High
    Intensity: Strong
    Quantity:>75%
    Spermatogonia cells
    Staining:Medium
    Intensity: Moderate
    Quantity:>75%)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2891/7680_A_4_6.jpg
    Figure 11 Lymph node
    Figure 11 Lymph node
    (Germinal center cells
    Staining:High
    Intensity: Strong
    Quantity:>75%
    Location: Nuclear
    Non-germinal center cells
    Staining:High
    Intensity: Strong
    Quantity:>75%
    Location: Nuclear)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2891/7680_A_8_8.jpg
    RNA Expression
    Figure 12 RNA cell line category: Cell line enhanced (MCF7)
    Figure 12 RNA cell line category: Cell line enhanced (MCF7)
    (Cell lines ordered by descending RNA expression order.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000142208-AKT1

    Specifications

    • Immunogen
    • Recombinant human AKT1-GST fusion protein
    • Host Species
    • Human
    • Type
    • Human IgG
    • Specificity
    • Human AKT1
    • Species Reactivity
    • Human
    • Clone
    • clone 30769
    • Applications
    • Western Blot
    • Related Disease
    • Proteus Syndrome; Cowden Syndrome 6

    Product Property

    • Purification
    • Affinity purified
    • Buffer
    • PBS
    • Preservative
    • 0.01% Thiomersal
    • Storage
    • Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

    Applications

    • Application Notes
    • Western blot: 1/200-1/2000

    Target

    • Alternative Names
    • AKT; PKB; RAC; PRKBA; PKB-ALPHA; RAC-ALPHA

    Recommended Products

    Isotype Control

    Recommended Dilution Buffer

    Secondary Antibody

    Related Resources

    • Related Signaling Pathways
    • Related Diseases

    Product Notes

    This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

    • Increased sensitivity
    • Confirmed specificity
    • High repeatability
    • Excellent batch-to-batch consistency
    • Sustainable supply
    • Animal-free production

    See more details about Hi-Affi™ recombinant antibody benefits.

    Downloads

    Download resources about recombinant antibody development and antibody engineering to boost your research.

    See other products for "AKT1"

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    CAT Product Name Application Type
    NAB-33-VHH Recombinant Anti-human AKT1 VHH Single Domain Antibody WB, IP, ChiP, Neut, ELISA Llama VHH

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