Human Anti-CEACAM5 Recombinant Antibody (clone CB3)

Name: Human Anti-CEACAM5 Recombinant Antibody (clone CB3)
SKU: VS-0724-XY74
Rating: 5 (1 reviews)

CAT#: VS-0724-XY74

Provided is a recombinant human antibody binding to CEACAM5 (CEA cell adhesion molecule 5), which represents the founding member of the carcinoembryonic antigen (CEA) family of proteins. It has been determined by Enzyme-linked Immunosorbent Assay.

Gene Expression

Subcellular Location Figure 1 IF staining of human cell line HaCaT

Immunofluorescent staining of human cell line HaCaT shows localization to plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19758/1660_G5_2_selected.jpg

Subcellular Location Figure 2 IHC staining of human duodenum

Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in a subset of leukocytes.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/11041/ihc_selected.jpg

Normal Tissue Figure 3 Colon

Endocrine cells
Staining: High
Intensity: Strong
Enterocytes
Staining: High
Intensity: Strong
Quantity:>75%
Enterocytes - Gradient
Gradient: Ascending
Enterocytes - Microvilli
Staining: High
Intensity: Strong
Goblet cells
Staining: High
Intensity: Strong
Quantity:>75%
Mucosal lymphoid cells
Staining: Medium
Intensity: Strong
Quantity: <25%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/21/19_A_7_3.jpg

Normal Tissue Figure 4 Rectum

Endocrine cells
Staining: High
Intensity: Strong
Enterocytes
Staining: High
Intensity: Strong
Quantity:>75%
Enterocytes - Microvilli
Staining: High
Intensity: Strong
Goblet cells
Staining: High
Intensity: Strong
Quantity:>75%
Mucosal lymphoid cells
Staining: Medium
Intensity: Strong
Quantity: <25%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/21/19_A_6_3.jpg

RNA Expression Figure 5 RNA cell line category: Cell line enriched (OE19)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000105388-CEACAM5

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Specifications

Host Species
Human

Type
Human IgG

Specificity
CEACAM5

Species Reactivity
Human

Clone
CB3

Applications
ELISA

Related Disease
Lung Non-Small Cell Carcinoma; Bile Duct Cancer

Product Property

Purification
Protein A affinity purified

Purity
>95%, determined by SDS-PAGE

Format
Liquid

Buffer
PBS

Preservative
No preservatives

Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Applications

Application Notes
ELISA: Various quantities of anti-CEACAM5 soluble antibody CB3 in 100 uL of blocking buffer were added to the wells and incubated for 30 min at 37°C.

Target

Alternative Names
CEA; CD66e

Gene ID
1048

UniProt ID
P06731

Cellular Localization
Cell membrane; Apical cell membrane

Post Translation Modifications
Glycosylation at Asn104, Asn115, Asn152, Asn182, Asn197, Asn204, Asn208, Asn246, Asn256, Asn274, Asn288, Asn292, Asn309, Asn330, Asn351, Asn360, Asn375, Asn432, Asn466, Asn480, Asn508, Asn529, Asn553, Asn560, Asn580, Asn612, Asn650, and Asn665.

Protein Refseq
XP_011524624.1; XP_016881634.1; XP_016881635.1

Function
Cell surface glycoprotein that plays a role in cell adhesion, intracellular signaling and tumor progression.
Mediates homophilic and heterophilic cell adhesion with other carcinoembryonic antigen-related cell adhesion molecules, such as CEACAM6.

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - VS-0724-XY74.pdf

MSDS

MSDS - VS-0724-XY74.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

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TAB-411LC-S(P)	Human Anti-Human CEACAM5 Recombinant Antibody scFv Fragment	ELISA	Human antibody
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HPAB-0155-FY-F(E)	Mouse Anti-CEACAM5 Recombinant Antibody; Fab Fragment (HPAB-0155-FY-F(E))	Activ	Humanized Fab
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HPAB-1094WJ-F(E)	Mouse Anti-CEACAM5 Recombinant Antibody; Fab Fragment (HPAB-1094WJ-F(E))	ELISA, FC	Mouse Fab
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VS3-CJ445	Mouse Anti-CEACAM5 Recombinant Antibody (VS3-CJ445)	WB	Mouse IgG
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