Rabbit Anti-CREB1 Recombinant Antibody (VS3-CJ694)
CAT#: VS3-CJ694
This product is a rabbit antibody that recognizes human, mouse, rat, and zebrafish CREB1.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19150/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3803/if_selected.jpg
Normal Tissue
Figure 3 IHC staining of human small intestine
Immunohistochemical staining of human small intestine shows strong nuclear positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19150/ihc_selected.jpg
Normal Tissue
Figure 4 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19150/44743_A_7_8.jpg
Normal Tissue
Figure 5 Cerebral cortex
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Glial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19150/44743_B_9_5.jpg
Normal Tissue
Figure 6 Colon
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19150/44743_A_9_3.jpg
Normal Tissue
Figure 7 Liver
Cholangiocytes Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19150/44743_A_7_4.jpg
Normal Tissue
Figure 8 Kidney
Cells in glomeruli Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Cells in tubules Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19150/44743_A_8_5.jpg
Normal Tissue
Figure 9 Testis
Leydig cells Staining: High Intensity: Strong Quantity: 75%-25% Peritubular cells Staining: High Intensity: Strong Quantity:>75% Round or early spermatids Staining: High Intensity: Strong Quantity:>75% Sertoli cells Staining: High Intensity: Strong Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19150/44743_A_6_6.jpg
RNA Expression
Figure 10 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000118260-CREB1
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Specifications
- Immunogen
- Recombinant protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse, Rat, Zebrafish CREB1
- Species Reactivity
- Human, Mouse, Rat, Zebrafish
- Applications
- WB, ICC, IF, IHC, IP, FC
- Conjugate
- Unconjugated
Product Property
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Buffer
- 40% Glycerol, 1% BSA, TBS, pH7.4.
- Preservative
- 0.05% Sodium Azide
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Flow Cytometry.
Target
- Alternative Names
- CREB; CREB-1
- Gene ID
- 1385
- UniProt ID
- P16220
- Sequence Similarities
- Belongs to the bZIP family.
- Cellular Localization
- Nucleus
- Post Translation Modifications
- Stimulated by phosphorylation. Phosphorylation of both Ser-119 and Ser-128 in the SCN regulates the activity of CREB and participates in circadian rhythm generation. Phosphorylation of Ser-119 allows CREBBP binding. In liver, phosphorylation is induced by fasting or glucagon in a circadian fashion (By similarity).
CREBL2 positively regulates phosphorylation at Ser-119 thereby stimulating CREB1 transcriptional activity (By similarity).
Phosphorylated upon calcium influx by CaMK4 and CaMK2 on Ser-119. CaMK4 is much more potent than CaMK2 in activating CREB. Phosphorylated by CaMK2 on Ser-128. Phosphorylation of Ser-128 blocks CREB-mediated transcription even when Ser-119 is phosphorylated. Phosphorylated by CaMK1 (By similarity).
Phosphorylation of Ser-257 by HIPK2 in response to genotoxic stress promotes CREB1 activity, facilitating the recruitment of the coactivator CBP. Phosphorylated at Ser-119 by RPS6KA3, RPS6KA4 and RPS6KA5 in response to mitogenic or stress stimuli. Phosphorylated by TSSK4 on Ser-119 (PubMed:15964553).
Sumoylated with SUMO1. Sumoylation on Lys-290, but not on Lys-271, is required for nuclear localization of this protein. Sumoylation is enhanced under hypoxia, promoting nuclear localization and stabilization.
- Protein Refseq
- NP_004370.1; NP_604391.1
- Function
- Phosphorylation-dependent transcription factor that stimulates transcription upon binding to the DNA cAMP response element (CRE), a sequence present in many viral and cellular promoters. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-133 phosphorylation. Involved in different cellular processes including the synchronization of circadian rhythmicity and the differentiation of adipose cells.
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Related Signaling Pathways
cAMP Signaling Pathway
MAPK Signaling Pathway
PPAR Signaling Pathway
TLR Signaling Pathway
TNF Signaling Pathway
Related Diseases
Prostate Cancer
Morphine Addiction
Cocaine Addiction
Amphetamine Addiction
Alcoholism
Cushing Syndrome
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "CREB1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0399CT | Recombinant Mouse anti-Human CREB1 Monoclonal antibody (93C625) | WB | |
| MRO-0388-CN | Rabbit Anti-CREB1 Recombinant Antibody (clone CBACN-159) | WB, IF, IHC, IP, FC | Rabbit IgG |
| MRO-1788-CN | Rabbit Anti-CREB1 Polyclonal Antibody (MRO-1788-CN) | WB, IF, IHC, FC | Rabbit IgG |
| VS3-FY413 | Recombinant Rabbit Anti-CREB1 Antibody (clone R08-4H9) | WB, IHC-P, IP | Rabbit IgG |
| VS3-FY2928 | Rabbit Anti-CREB1 (pSer133) Recombinant Antibody (VS3-FY2928) | WB, IHC, ELISA | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0781 | Hi-Affi™ Rabbit Anti-CREB1 Recombinant Antibody (clone DS781AB) | WB, IP | Rabbit IgG |
| MOR-0800 | Hi-Affi™ Rabbit Anti-CREB1 Recombinant Antibody (clone DS800AB) | WB, ICC, IF, IHC-P, FC, IP | Rabbit IgG |
| MOR-4560 | Hi-Affi™ Rabbit Anti-CREB1 Recombinant Antibody (clone TH70DS) | WB, IF, ICC, FC, ChIP | Rabbit IgG |
| MOR-4561 | Hi-Affi™ Rabbit Anti-CREB1 Recombinant Antibody (clone TH71DS) | WB, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY69 | AbPlus™ Anti-CREB1 Magnetic Beads (CBACN-159) | IP, Protein Purification | |
| VS-0724-YC812 | AbPlus™ Anti-CREB1 Magnetic Beads (VS-0724-YC812) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY538 | Anti-CREB1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1663 | Anti-Mouse CREB1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1662 | Anti-Human CREB1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1664 | Anti-Rat CREB1 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC244 | CytoStream™ Rabbit Anti-CREB1 Recombinant Antibody (VS13-YC244) | WB, ICC, IF, IHC-P, IP, FC | Rabbit IgG |
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