Human Anti-CXCR4 Recombinant Antibody (clone Ab124) (CAT#: HPAB-1842-FY)
This product is a recombinant antibody that recognizes CXCR4. The monoclonal antibody Ab124 reacts with the epitope of CXCR4. Ab124 can inhibit SDF-1 induced chemotaxis in human breast cancer line MDA-MB-231.
Specific Inquiry
ELISA
Figure 1 Anti-CXCR4 scFv-binding assays.
ELISA-binding assays were performed to measure anti-CXCR4 scFv (Ab124; Ab125) binding to PC-3 breast cancer cells.
Vaday, G. G., Hua, S. B., Peehl, D. M., Pauling, M. H., Lin, Y. H., Zhu, L., ... & Zucker, S. (2004). CXCR4 and CXCL12 (SDF-1) in prostate cancer: inhibitory effects of human single chain Fv antibodies. Clinical cancer research, 10(16), 5630-5639.
FC
Figure 2 Anti-CXCR4 scFv binding to PCa cells.
Flow cytometric analysis was performed using a three-step procedure. Cells were first incubated with Ab16, Ab124, or Ab125 (10 μg/ml) or without scFv, followed by anti-HSV Tag mAb (0.5 μg/ml), then fluorescent antimouse IgG antibody.
Vaday, G. G., Hua, S. B., Peehl, D. M., Pauling, M. H., Lin, Y. H., Zhu, L., ... & Zucker, S. (2004). CXCR4 and CXCL12 (SDF-1) in prostate cancer: inhibitory effects of human single chain Fv antibodies. Clinical cancer research, 10(16), 5630-5639.
Inhib
Figure 3 Anti-CXCR4 scFv inhibit CXCL12-induced calcium flux.
The effects of Ab124 and Ab125 on calcium mobilization in PCa cells was determined. PC-3 and LNCaP cells were preincubated with Ab16, Ab124, or Ab125 (10 μg/ml) for 30 min, then exposed to CXCL12 (100 ng/ml) for measurement of calcium flux.
Vaday, G. G., Hua, S. B., Peehl, D. M., Pauling, M. H., Lin, Y. H., Zhu, L., ... & Zucker, S. (2004). CXCR4 and CXCL12 (SDF-1) in prostate cancer: inhibitory effects of human single chain Fv antibodies. Clinical cancer research, 10(16), 5630-5639.
Inhib
Figure 4 Anti-CXCR4 scFv inhibit CXCL12-induced chemotaxis.
Chemotaxis assays were performed to determine the inhibitory effects of Ab124 and Ab125 on CXCL12-induced migration of PCa cells and control T cells. PC-3 cells were preincubated with Ab16, Ab124, or Ab125 (10 μg/ml) for 30 min at 37°C, then incubated in Transwell migration chambers containing CXCL12 (400 ng/ml) for 18–24 h.
Vaday, G. G., Hua, S. B., Peehl, D. M., Pauling, M. H., Lin, Y. H., Zhu, L., ... & Zucker, S. (2004). CXCR4 and CXCL12 (SDF-1) in prostate cancer: inhibitory effects of human single chain Fv antibodies. Clinical cancer research, 10(16), 5630-5639.
Specifications
- Host Species
- Human
- Type
- Human IgG
- Specificity
- Human CXCR4
- Species Reactivity
- Human
- Clone
- Ab124
- Applications
- ELISA, FC, Inhib
- Related Disease
- Breast cancer
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The CXCR4 antibody has been reported in applications of Enzyme-linked Immunosorbent Assay, Flow Cytometry, Inhibition.
FC: 10 μg/ml.
Inhibition: 10 μg/ml.
Target
- Alternative Names
- C-X-C Motif Chemokine Receptor 4; Leukocyte-Derived Seven Transmembrane Domain Receptor; Lipopolysaccharide-Associated Protein 3; Stromal Cell-Derived Factor 1 Receptor; Chemokine (C-X-C Motif) Receptor 4; LPS-Associated Protein 3; SDF-1 Receptor; CD184 Antigen; Fusin; LAP-3; LESTR; NPYRL; FB22; HM89; LCR1; Seven-Transmembrane-Segment Receptor, Spleen; Chemokine (C-X-C Motif), Receptor 4 (Fusin)
- Gene ID
- 7852
- UniProt ID
- P61073
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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Mouse Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0314CL | Mouse Anti-CXCR4 Recombinant Antibody (TAB-0314CL) | FC | Mouse IgG |
| TAB-0314CL-F(E) | Mouse Anti-CXCR4 Recombinant Antibody; Fab Fragment (TAB-0314CL-F(E)) | FC | Mouse Fab |
| TAB-143MZ-S(P) | Mouse Anti-CXCR4 Recombinant Antibody; scFv Fragment (TAB-143MZ-S(P)) | cAMP Assay | Mouse scFv |
| TAB-144MZ-S(P) | Mouse Anti-CXCR4 Recombinant Antibody; scFv Fragment (TAB-144MZ-S(P)) | cAMP Assay | Mouse scFv |
| TAB-145MZ-S(P) | Mouse Anti-CXCR4 Recombinant Antibody; scFv Fragment (TAB-145MZ-S(P)) | cAMP Assay | Mouse scFv |
Humanized Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-146MZ | Human Anti-CXCR4 Recombinant Antibody (TAB-146MZ) | Cyt, FuncS, Inhib | Humanized IgG1 |
| TAB-147MZ | Human Anti-CXCR4 Recombinant Antibody (TAB-147MZ) | Cyt, FuncS, Inhib | Humanized IgG1 |
| TAB-146MZ-S(P) | Human Anti-CXCR4 Recombinant Antibody; scFv Fragment (TAB-146MZ-S(P)) | cAMP Assay | Humanized scFv |
| TAB-147MZ-S(P) | Human Anti-CXCR4 Recombinant Antibody; scFv Fragment (TAB-147MZ-S(P)) | cAMP Assay | Humanized scFv |
| TAB-148MZ-S(P) | Human Anti-CXCR4 Recombinant Antibody; scFv Fragment (TAB-148MZ-S(P)) | cAMP Assay | Humanized scFv |
Human Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-150MZ-F(E) | Anti-Human CXCR4 Recombinant Antibody Fab Fragment (CF172) | FC | Human antibody |
| TAB-151MZ-F(E) | Anti-Human CXCR4 Recombinant Antibody Fab Fragment (5C9) | WB, ELISA | Human antibody |
| TAB-152MZ-F(E) | Anti-Human CXCR4 Recombinant Antibody Fab Fragment (5E1) | WB, ELISA | Human antibody |
| TAB-153MZ-F(E) | Anti-Human CXCR4 Recombinant Antibody Fab Fragment (7C8) | WB, ELISA | Human antibody |
| TAB-154MZ-F(E) | Anti-Human CXCR4 Recombinant Antibody Fab Fragment (6C7) | WB, ELISA | Human antibody |
Chicken IgY Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0152MZ | Chicken Anti-CXCR4 Polyclonal IgY | WB | Chicken antibody |
Neutralizing Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-684CQ | Mouse Anti-CXCR4 Recombinant Antibody (clone 12G5) | FC, CyTOF®, Neut | Mouse IgG2a |
| NEUT-685CQ | Mouse Anti-CXCR4 Recombinant Antibody (clone 12G5R) | FC, Neut | Mouse IgG2a |
| NEUT-687CQ | Mouse Anti-CXCR4 Recombinant Antibody (clone CBL046) | FC, IHC, Neut | Mouse IgG2 |
| NEUT-688CQ | Mouse Anti-CXCR4 Recombinant Antibody (clone CBL470) | FC, CyTOF®, Neut | Mouse IgG2b |
| NEUT-689CQ | Mouse Anti-CXCR4 Recombinant Antibody (clone CBL783) | Neut | Mouse IgG2a |
Blocking Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-686CQ | Mouse Anti-CXCR4 Recombinant Antibody (clone 44716.111) | FC, ICC, IF, IHC-P, Inhib | Mouse IgG2b |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0889 | Hi-Affi™ Rabbit Anti-CXCR4 Recombinant Antibody (clone DS889AB) | WB, IHC | Rabbit IgG |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0044-YC-S(P) | Human Anti-CXCR4 Recombinant Antibody (clone hz515H7VH1D76NVL2); scFv Fragment | Block, Cyt, FC | Humanized scFv |
| HPAB-0045-YC-S(P) | Human Anti-CXCR4 Recombinant Antibody; scFv Fragment (HPAB-0045-YC-S(P)) | FC, Cyt | Humanized scFv |
| HPAB-1843-FY-F(E) | Human Anti-CXCR4 Recombinant Antibody (clone Ab125); scFv Fragment | Inhib | Human scFv |
| HPAB-2066-FY-S(P) | Human Anti-CXCR4 Recombinant Antibody; scFv Fragment (HPAB-2066-FY-S(P)) | FC, ELISA | Human scFv |
| HPAB-2067-FY-S(P) | Human Anti-CXCR4 Recombinant Antibody; scFv Fragment (HPAB-2067-FY-S(P)) | FC, ELISA | Human scFv |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1796-CN | Rabbit Anti-CXCR4 Polyclonal Antibody (MRO-1796-CN) | WB, IF, IHC, FC | Rabbit IgG |
| FN-163CQ | Rat Anti-Cxcr4 Recombinant Antibody (clone CBL-1324) | FC, FuncS | Rat IgG2b, κ |
| HPAB-1504-FY | Human Anti-CXCR4 Recombinant Antibody (clone C-9P21) | ELISA, Inhib | Human IgG1 |
| HPAB-1505-FY | Human Anti-CXCR4 Recombinant Antibody (clone B-1M2) | ELISA, Inhib | Human IgG1 |
| HPAB-1506-FY | Human Anti-CXCR4 Recombinant Antibody (clone C-1I24) | ELISA, Inhib | Human IgG1 |
ADCC Enhanced Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-H72 | Afuco™ Anti-CXCR4 ADCC Recombinant Antibody (Ulocuplumab), ADCC Enhanced | IP, IF, FuncS, FC, Neut, ELISA | ADCC enhanced antibody |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0329-WJ-F(E) | Mouse Anti-CXCR4 Recombinant Antibody; Fab Fragment (HPAB-0329-WJ-F(E)) | ELISA, FC, IHC | Mouse Fab |
| HPAB-0334-WJ-F(E) | Mouse Anti-CXCR4 Recombinant Antibody (clone 4C1); Fab Fragment | ELISA, WB | Mouse Fab |
| HPAB-0335-WJ-F(E) | Mouse Anti-CXCR4 Recombinant Antibody (clone 6C7); Fab Fragment | ELISA, WB | Mouse Fab |
| HPAB-0336-WJ-F(E) | Mouse Anti-CXCR4 Recombinant Antibody (clone 2A4); Fab Fragment | ELISA, WB | Mouse Fab |
| HPAB-0337-WJ-F(E) | Mouse Anti-CXCR4 Recombinant Antibody (clone 5C9); Fab Fragment | ELISA, WB | Mouse Fab |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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