Rabbit Anti-CXCR4 Recombinant Antibody (clone R08-8J4)
CAT#: VS3-XY3329
This antibody is a recombinant rabbit antibody targeting the CXCR4 (C-X-C motif chemokine receptor 4), which involves in hematopoiesis and in cardiac ventricular septum formation. It specifically reacts with human CXCR4. It has been reported for use in Western Blot, Immunohistochemistry-Paraffin, Immunocytochemistry or Immunofluorescence. The recommended dilutions of Western Blot, Immunohistochemistry-Paraffin and Immunofluorescence is 1/500-1/1000, 1/50-1/100 and 1/50-1/200, respectively.
Gene Expression
RNA Expression
Figure 1 RNA cell line category: Cell line enhanced (Daudi, JURKAT, Karpas-707, MOLT-4, REH, U-266/70, U-698)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000121966-CXCR4
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Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Mouse CXCR4
- Species Reactivity
- Human, Mouse
- Clone
- R08-8J4
- Applications
- WB, IHC-P, ICC, IF
- Related Disease
- Whim Syndrome 1; Macroglobulinemia
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IHC: 1/50-1/100
IF: 1/50-1/200
Target
- Alternative Names
- FB22; HM89; LAP3; LCR1; NPYR; WHIM; CD184; LAP-3; LESTR; NPY3R; NPYRL; WHIMS; HSY3RR; NPYY3R; WHIMS1; D2S201E
- Gene ID
- 7852
- UniProt ID
- P61073
- Long Name
- C-X-C motif chemokine receptor 4
- Cellular Localization
- Cell membrane, Cell junction
- Post Translation Modifications
- Glycosylation at Asn11, Ser18, and Asn176 (NX_P61073 [NX_P61073-1])
Ubiquitination at Lys310, Lys327, and Lys331 (NX_P61073 [NX_P61073-1])
Modification sites at PhosphoSitePlus (P61073)
Glycosylation from GlyGen (P61073) 3 sites
- Protein Refseq
- XP_047301758.1; NP_001008540.1
- Function
- Involved in the AKT signaling cascade. Plays a role in regulation of cell migration, e.g.during wound healing. Acts as a receptor for extracellular ubiquitin; leading to enhanced intracellular calcium ions and reduced cellular cAMP levels.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
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