Recombinant Llama Anti-DLL4 Single Domain Antibody (HPAB-1512-FY)
CAT#: HPAB-1512-FY
This product is a recombinant single domain antibody that recognizes DLL4. The monoclonal single domain antibody 56A09 reacts with the epitope of DLL4. DII4-mediated inhibition of HUVEC proliferation.
Published Data
Gene Expression
Block
Figure 1 Purified VHH (anti-DLL4 single domain antibody (56A09)) block the hDLL4/hNotch1 -Fc interaction (ELISA).
The blocking capacity of the VHHs is evaluated in a human DII4 - human Notch1/Fc blocking ELISA. In brief, 1 μg/mL of human Notch1/Fc chimera is coated in a 96-well MaxiSorp plate. A fixed concentration of 15 nM biotinylated human DII4 is preincubated with a dilution series of the VHH for 1 hour, after which the mixture is incubated on the coated Notchi receptor for an additional hour. Residual binding of biotinylated human DII4 is detected using horseradish peroxidase (HRP) conjugated extravidin.
Block
Figure 2 Purified VHH (anti-DLL4 single domain antibody (56A09)) block the hDLL4/hNotch1 -Fc interaction (ELISA).
The blocking capacity of the VHHs is evaluated in a human DII4 - human Notch1/Fc blocking ELISA. In brief, 1 μg/mL of human Notch1/Fc chimera is coated in a 96-well MaxiSorp plate. A fixed concentration of 15 nM biotinylated human DII4 is preincubated with a dilution series of the VHH for 1 hour, after which the mixture is incubated on the coated Notchi receptor for an additional hour. Residual binding of biotinylated human DII4 is detected using horseradish peroxidase (HRP) conjugated extravidin.
ELISA
Figure 3 Binding of purified VHH (anti-DLL4 single domain antibody (56A09)) to recombinant human and mouse DLL4 (ELISA).
Recombinant mouse DII4 is coated overnight at 4°C at 1 μg/mL in a 96-well MaxiSorp plate (Nunc, Wiesbaden, Germany). Wells are blocked with a casein solution (1 % in PBS). VHHs are applied as dilution series and binding is detected using a mouse anti-myc (Roche) and an anti-mouse-AP conjugate (Sigma, St Louis, MO, USA).
ELISA
Figure 4 Binding of purified VHH (anti-DLL4 single domain antibody (56A09)) to recombinant human and mouse DLL4 (ELISA).
Recombinant mouse DII4 is coated overnight at 4°C at 1 μg/mL in a 96-well MaxiSorp plate (Nunc, Wiesbaden, Germany). Wells are blocked with a casein solution (1 % in PBS). VHHs are applied as dilution series and binding is detected using a mouse anti-myc (Roche) and an anti-mouse-AP conjugate (Sigma, St Louis, MO, USA).
FC
Figure 5 Binding of purified VHHs to human/mouse/cynomolgus DLL4 (FACS).
Cynomolgus DII4 expressing HEK293 cells (transient or stable transfection) are used for a titration binding experiment of the VHHs. After a 30 minutes incubation on ice, all samples are washed and detection is performed by applying anti-c-myc~Alexa647 (Santa Cruz Biotechnology, Santa Cruz, CA, USA). Human and mouse DII4 overexpressing HEK293 cells are taken as reference. The mean MCF value is determined on the FACS Array and used for calculation of the EC50 value.
FC
Figure 6 Binding of purified VHHs to human/mouse/cynomolgus DLL4 (FACS).
Cynomolgus DII4 expressing HEK293 cells (transient or stable transfection) are used for a titration binding experiment of the VHHs. After a 30 minutes incubation on ice, all samples are washed and detection is performed by applying anti-c-myc~Alexa647 (Santa Cruz Biotechnology, Santa Cruz, CA, USA). Human and mouse DII4 overexpressing HEK293 cells are taken as reference. The mean MCF value is determined on the FACS Array and used for calculation of the EC50 value.
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Normal Tissue
Figure 1 IHC staining of human small intestine
Immunohistochemical staining of human small intestine shows strong membranous positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/23392/51479_A_4_2_selected.jpg
Normal Tissue
Figure 2 Colon
Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/23392/51479_A_9_3.jpg
Normal Tissue
Figure 3 Liver
Cholangiocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/23392/51479_A_8_4.jpg
Normal Tissue
Figure 4 Kidney
Cells in tubules Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/23392/51479_A_8_5.jpg
Normal Tissue
Figure 5 Testis
Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/23392/51479_A_4_6.jpg
RNA Expression
Figure 6 RNA cell line category: Group enriched (BJ hTERT+, HUVEC TERT2, SCLC-21H)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000128917-DLL4
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Specifications
- Host Species
- Llama
- Type
- Llama VHH
- Specificity
- Human DLL4
- Species Reactivity
- Human, Mouse
- Clone
- HPAB-1512-FY
- Applications
- Block, ELISA, FC
Product Property
- Purity
- >95% as determined by analysis by SDS-PAGE
- Storage
- Store at -20°C for long-term storage. Avoid freeze/thaw cycles.
Applications
- Application Notes
- The DLL4 antibody has been reported in applications of Blocking, Enzyme-linked Immunosorbent Assay, Flow Cytometry.
Target
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Properly Packed
The antibody was packaged safely, arrived undamaged, and included all necessary information. When I arrived, the condition was excellent.
Verifying Band Designs
Western blots using this antibody provide clear, consistent band patterns, which are confirmatory. It does simplify the process of interpreting the data.
Effective when using secondary antibodies
This antibody produces strong and targeted signals in conjugated studies because of its excellent compatibility with a wide range of secondary antibodies.
Q&As
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How should I store this antibody for best results?
A: You should store the antibody between -20°C and -80°C. Repetitive freeze-thaw cycles should be avoided since they can weaken the antibody and decrease its effectiveness in tests.
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What's the concentration of this antibody solution?
A: Generally, the antibody concentration is 1 mg/mL; however, for exact information specific to the lot you are using, it is always preferable to consult the product datasheet.
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Are there any studies that have used this antibody?
A: Yes, this antibody has been used in a number of peer-reviewed articles. For a detailed list, visit our website's product page.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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| HPAB-0204-CN-S(P) | Human Anti-DLL4 Recombinant Antibody; scFv Fragment (HPAB-0204-CN-S(P)) | ELISA, WB, FC, IP | Human scFv |
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| HPAB-0206-CN-S(P) | Mouse Anti-DLL4 Recombinant Antibody; scFv Fragment (HPAB-0206-CN-S(P)) | ELISA, WB, FC, IHC, IF, ICC | Mouse scFv |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
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| HPAB-0207-CN-F(E) | Human Anti-DLL4 Recombinant Antibody; Fab Fragment (HPAB-0207-CN-F(E)) | ELISA, WB, FC, IHC, IF, ICC | Chimeric (mouse/human) Fab |
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