Recombinant Llama Anti-DLL4 Single Domain Antibody (HPAB-1514-FY)

Name: Recombinant Llama Anti-DLL4 Single Domain Antibody (HPAB-1514-FY)
SKU: HPAB-1514-FY
Rating: 5 (1 reviews)

CAT#: HPAB-1514-FY

This product is a recombinant single domain antibody that recognizes DLL4. The monoclonal single domain antibody 104G01 reacts with the epitope of DLL4. DII4-mediated inhibition of HUVEC proliferation.

Published Data Gene Expression

Block

Figure 1 Purified VHH (anti-DLL4 single domain antibody (104G01)) block the hDLL4/hNotch1 -Fc interaction (ELISA).

The blocking capacity of the VHHs is evaluated in a human DII4 - human Notch1/Fc blocking ELISA. In brief, 1 μg/mL of human Notch1/Fc chimera is coated in a 96-well MaxiSorp plate. A fixed concentration of 15 nM biotinylated human DII4 is preincubated with a dilution series of the VHH for 1 hour, after which the mixture is incubated on the coated Notchi receptor for an additional hour. Residual binding of biotinylated human DII4 is detected using horseradish peroxidase (HRP) conjugated extravidin.

Block

Figure 2 Purified VHH (anti-DLL4 single domain antibody (104G01)) block the CHO-hDLL4/hNotch1-Fc and CHO-mDLL4/hNotch1 -Fc interaction (FMAT).

Inhibition by anti-DII4 VHHs of human Notch 1/Fc binding to human or mouse DII4 expressed on the CHO cells.The blocking capacity of the VHHs is evaluated in a human and mouse DII4 - human Notch 1/Fc competitive FMAT assay.

ELISA

Figure 3 Binding of purified VHH (anti-DLL4 single domain antibody (104G01)) to recombinant human and mouse DLL4 (ELISA).

Recombinant mouse DII4 is coated overnight at 4°C at 1 μg/mL in a 96-well MaxiSorp plate (Nunc, Wiesbaden, Germany). Wells are blocked with a casein solution (1 % in PBS). VHHs are applied as dilution series and binding is detected using a mouse anti-myc (Roche) and an anti-mouse-AP conjugate (Sigma, St Louis, MO, USA).

ELISA

Figure 4 Binding of purified VHH (anti-DLL4 single domain antibody (104G01)) to recombinant human and mouse DLL4 (ELISA).

Figure 5 Binding of purified VHHs to human/mouse/cynomolgus DLL4 (FACS).

Cynomolgus DII4 expressing HEK293 cells (transient or stable transfection) are used for a titration binding experiment of the VHHs. After a 30 minutes incubation on ice, all samples are washed and detection is performed by applying anti-c-myc~Alexa647 (Santa Cruz Biotechnology, Santa Cruz, CA, USA). Human and mouse DII4 overexpressing HEK293 cells are taken as reference. The mean MCF value is determined on the FACS Array and used for calculation of the EC50 value.

Figure 6 Binding of purified VHHs to human/mouse/cynomolgus DLL4 (FACS).

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Normal Tissue Figure 1 IHC staining of human small intestine

Immunohistochemical staining of human small intestine shows strong membranous positivity in glandular cells.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/23392/51479_A_4_2_selected.jpg

Normal Tissue Figure 2 Colon

Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/23392/51479_A_9_3.jpg

Normal Tissue Figure 3 Liver

Cholangiocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/23392/51479_A_8_4.jpg

Normal Tissue Figure 4 Kidney

Cells in tubules Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/23392/51479_A_8_5.jpg

Normal Tissue Figure 5 Testis

Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/23392/51479_A_4_6.jpg

RNA Expression Figure 6 RNA cell line category: Group enriched (BJ hTERT+, HUVEC TERT2, SCLC-21H)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000128917-DLL4

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Specifications

Host Species
Llama

Type
Llama VHH

Antibody Isotype
VHH

Specificity
Human DLL4

Species Reactivity
Human, Mouse

Clone
HPAB-1514-FY

Applications
Block, ELISA, FC

Product Property

Purity
>95% as determined by analysis by SDS-PAGE

Storage
Store at -20°C for long-term storage. Avoid freeze/thaw cycles.

Applications

Application Notes
The DLL4 antibody has been reported in applications of Blocking, Enzyme-linked Immunosorbent Assay, Flow Cytometry.

Target

Alternative Names
Delta Like Canonical Notch Ligand 4; Drosophila Delta Homolog 4; Delta4; Delta-Like 4 Homolog (Drosophila); Delta-Like 4 (Drosophila); Delta-Like 4 Homolog; Delta-Like 4 Protein; Delta-Like Protein 4

Gene ID
54567

UniProt ID
Q9NR61

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - HPAB-1514-FY.pdf

MSDS

MSDS - HPAB-1514-FY.pdf

COA

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