Anti-FAS Antibody Prodrug, Protease Activated (h-HFE7A)

Name: Anti-FAS Antibody Prodrug, Protease Activated (h-HFE7A)
SKU: VS-1025-YC68
Rating: 5 (1 reviews)

CAT#: VS-1025-YC68

The Anti-FAS Antibody Prodrug, Protease Activated (h-HFE7A) is an antibody prodrug comprised of a recombinant human antibody targeting human FAS, linked to a masking peptide that conceals the active antigen-binding site via a protease-cleavable linker. The mask peptide is recombinantly attached to the amino terminus of either the light or heavy chain of the antibody, designed to block access to the antigen-binding site and physically prevent the antibody from binding to FAS protein. Once the masking peptide is cleaved by matrix metalloproteinases (MMPs), the prodrug is activated, allowing the unmasked monoclonal antibody component to bind to FAS effectively. FAS is a death receptor on the cell surface that, when activated by its ligand (FASL), initiates the extrinsic pathway of apoptosis. Many tumor cells develop mechanisms to inactivate or shed FAS, thus rendering them resistant to T-cell-mediated killing and other death signals. Maintaining this resistance is a key survival mechanism for the tumor.

Gene Expression

Subcellular Location Figure 1 IF staining of human cell line A-431

Immunofluorescent staining of human cell line A-431 shows localization to nuclear bodies, plasma membrane & cytosol.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/27444/if_selected.jpg

Subcellular Location Figure 2 IF staining of human cell line U-251 MG

Immunofluorescent staining of human cell line U-251 MG shows localization to nuclear bodies, plasma membrane & cytosol.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/27444/546_F3_1_blue_red_green.jpg

Normal Tissue Figure 3 Colon

Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/27444/61440_A_9_3.jpg

Normal Tissue Figure 4 Testis

Leydig cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/27444/61440_A_6_6.jpg

RNA Expression Figure 5 RNA cell line category: Cell line enhanced (HDLM-2, Karpas-707)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000026103-FAS

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Specifications

Host Animal
Human

Applications
In situ zymography (ISZ), Cytotoxicity assay (Cyt), Functional assay

Specificity
Human FAS

Target
FAS

Species Reactivity
Human

Isotype
IgG1, kappa

Clone
h-HFE7A

Masking Approach
Affinity mask

Mask
A synthetic peptide

Protease Cleavable Linker
MMP-cleavable linker

Protease Substrate
MMPs

Purification
Protein A purified

Buffer
PBS, pH 7.4

Preservative
No preservatives

Storage
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Target

Introduction
Fas cell surface death receptor is a member of the TNF-receptor superfamily. It contains a death domain. It has been shown to play a crucial role in the physiological regulation of programmed cell death and has been implicated in the pathogenesis of various malignancies and diseases of the immune system. The interaction of FAS with its ligand allows the formation of a death-inducing signaling complex that includes Fas-associated death domain protein (FADD), caspase 8, and caspase 10.

Alternative Names
CD95 Antigen; TNFRSF6; APT1; FAS1; R-125224; h-HFE7A

Gene ID
355

UniProt ID
P25445

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Related Signaling Pathways

TNF Signaling Pathway

Related Diseases

Allograft Rejection

Autoimmune Thyroid Disease

Graft-versus-Host Disease

Maturity Onset Diabetes of the Young

Downloadable Resources

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Datasheet

Datasheet - VS-1025-YC68.pdf

MSDS

MSDS - VS-1025-YC68.pdf

COA

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