Human Anti-FAS Recombinant Antibody (clone HFE7A)
CAT#: PABL-094
Recombinant Human Antibody (HFE7A) is capable of binding to Fas, expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-Fas mAb and CH1-3 region of human IgG1 and a light chain (LC) encoding VL from anti-Fas mAb and CL of human kappa light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition.
Published Data
Gene Expression
Figure 1 Binding Assay to Human Fas-AIC2.
The symbols correspond to h-HFE7A α- (◇), β- (□), γ- (〇), δ- (△), and ζ- (▼). Chimeric HFE7A (■) was used as the positive control. Human IgG1 (●) was used as the negative control. Similar results were obtained with three independent experiments.
Haruyama, H., Ito, S., Miyadai, K., Takahashi, T., Kawaida, R., Takayama, T.,... & Ichikawa, K. (2002). Humanization of the mouse anti-Fas antibody HFE7A and crystal structure of the humanized HFE7A Fab fragment. Biological and Pharmaceutical Bulletin, 25(12), 1537-1545.
Figure 2 Inhibition Assay against the Binding between m-HFE7A and Human Fas-AIC2.
The symbols correspond to h-HFE7A α- (◇), β- (□), γ- (〇), δ- (△), and ζ- (▼). Chimeric HFE7A (■) was used as the positive control. Human IgG1 (●) was used as the negative control. Similar results were obtained with three independent experiments.
Haruyama, H., Ito, S., Miyadai, K., Takahashi, T., Kawaida, R., Takayama, T.,... & Ichikawa, K. (2002). Humanization of the mouse anti-Fas antibody HFE7A and crystal structure of the humanized HFE7A Fab fragment. Biological and Pharmaceutical Bulletin, 25(12), 1537-1545.
Figure 3 Apoptosis Induction Assay against WR19L12a Cells.
The symbols correspond to h-HFE7A α- (◇), β- (□), γ- (〇), δ- (△), and ζ- (▼). Chimeric HFE7A (■) was used as the positive control. Human IgG1 (●) was used as the negative control. Similar results were obtained with three independent experiments.
Haruyama, H., Ito, S., Miyadai, K., Takahashi, T., Kawaida, R., Takayama, T.,... & Ichikawa, K. (2002). Humanization of the mouse anti-Fas antibody HFE7A and crystal structure of the humanized HFE7A Fab fragment. Biological and Pharmaceutical Bulletin, 25(12), 1537-1545.
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Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nuclear bodies, plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/27444/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nuclear bodies, plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/27444/546_F3_1_blue_red_green.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/27444/61440_A_9_3.jpg
Normal Tissue
Figure 4 Testis
Leydig cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/27444/61440_A_6_6.jpg
RNA Expression
Figure 5 RNA cell line category: Cell line enhanced (HDLM-2, Karpas-707)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000026103-FAS
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Specifications
- Immunogen
- Human Fas cell surface death receptor
- Host Species
- Human
- Type
- Human IgG
- Specificity
- Human FAS
- Species Reactivity
- Human
- Clone
- HFE7A
- Applications
- ELISA, WB, IF, FuncS
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Inhibition and Apoptosis. For details, refer to published data.
Target
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBL-094 | Human Anti-FAS Recombinant Antibody (clone HFE7A); scFv Fragment | ELISA, WB, IF, FuncS | Human scFv |
| PSBL-723 | Human Anti-FAS Recombinant Antibody (clone E09); scFv Fragment | WB, IF, FuncS | Human scFv |
| PSBL-724 | Human Anti-FAS Recombinant Antibody (clone EP6b-B01); scFv Fragment | WB, FuncS | Human scFv |
| HPAB-0450-YJ-S(P) | Rabbit Anti-FAS Recombinant Antibody; scFv Fragment (HPAB-0450-YJ-S(P)) | ELISA, FC | Rabbit scFv |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBL-094 | Human Anti-FAS Recombinant Antibody (clone HFE7A); Fab Fragment | ELISA, WB, IF, FuncS | Human Fab |
| PFBL-723 | Human Anti-FAS Recombinant Antibody (clone E09); Fab Fragment | WB, IF, FuncS | Human Fab |
| PFBL-724 | Human Anti-FAS Recombinant Antibody (clone EP6b-B01); Fab Fragment | WB, FuncS | Human Fab |
| HPAB-0035-YJ-F(E) | Human Anti-FAS Recombinant Antibody (clone h-HFE7A); Fab Fragment | ELISA, Cyt, Apop | Human Fab |
| HPAB-1575WJ-F(E) | Mouse Anti-FAS Recombinant Antibody; Fab Fragment (clone F919-9-18) | ELISA, WB | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABL-730 | Human Anti-FAS Recombinant Antibody (PABL-730) | WB, IF, FuncS | Human IgG |
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| BRD-0201MZ | Chicken Anti-FAS (ab1) Polyclonal IgY | WB | Chicken antibody |
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| CAT | Product Name | Application | Type |
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| NEUT-767CQ | Mouse Anti-FAS Recombinant Antibody (clone CBL933) | Neut, FC, WB | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-768CQ | Mouse Anti-FAS Recombinant Antibody (clone ZB4) | Block, FC, IHC, WB | Mouse IgG1 |
| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| FN-213CQ | Mouse Anti-FAS Recombinant Antibody (clone EOS9.1) | FC, FuncS | Mouse IgM, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0924-YC127 | Human Anti-FAS Recombinant Antibody Hexamer (VS-0924-YC127), CDC Enhanced | IHC, FC, ADCC, CDC | Antibody hexamer |
| VS-0924-YC128 | Mouse Anti-FAS Recombinant Antibody Hexamer (VS-0924-YC128), CDC Enhanced | ELISA, WB, CDC | Antibody hexamer |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC348 | Recombinant Anti-FAS Vesicular Antibody, EV Displayed (VS-0425-YC348) | ELISA, FC, Cell-uptake |
| CAT | Product Name | Application | Type |
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| VS-0525-XY2448 | Anti-FAS Immunohistochemistry Kit | IHC | |
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