Mouse Anti-FAS Recombinant Antibody (clone CH11)
CAT#: MOB-103LC
This product is a mouse anti-human FAS antibody with agonistic activity. The clone CH11 is a recombinant antibody that binds to human FAS and can be potentially used in the study of diseases that are therapeutically benificial from FAS-activation.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nuclear bodies, plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/27444/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nuclear bodies, plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/27444/546_F3_1_blue_red_green.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/27444/61440_A_9_3.jpg
Normal Tissue
Figure 4 Testis
Leydig cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/27444/61440_A_6_6.jpg
RNA Expression
Figure 5 RNA cell line category: Cell line enhanced (HDLM-2, Karpas-707)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000026103-FAS
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Specifications
- Immunogen
- Human diploid fibroblast cell line
- Host Species
- Mouse
- Specificity
- Human FAS
- Species Reactivity
- Human
- Clone
- CH11
- Applications
- FC, ICC, WB
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
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High Specificity
I'm really impressed with the specificity of this antibody. It consistently shows clear, specific bands in my Western blots without any background noise. Great product for protein analysis!
Ideal for Flow Cytometry
The antibody works perfectly in flow cytometry assays. It provides clear and specific signals without much optimization. Definitely a reliable reagent for cell analysis!
Easy-to-use
The antibody is easy to handle and prepare. The product arrived with detailed usage instructions and didn't need any additional modifications.
Q&As
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What benefits might recombinant antibodies offer?
A: Recombinant antibodies are more consistent between batches and have higher specificity and purity than traditional monoclonal or polyclonal antibodies.
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What actions should I take if the anticipated outcomes from my antibodies don't materialize?
A: To troubleshoot, go over the protocol's steps again, adjust antibody concentrations, ensure that the reagents are kept correctly, and make sure each reagent is functioning as it should. Consult technical support if issues persist.
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How can the stability of the antibody be best ensured throughout time?
A: Aliquoting the antibody, minimizing freeze-thaw cycles, and preserving optimal storage conditions can all help to improve stability. Follow the manufacturer's recommendation to use stabilizing agents.
View the frequently asked questions answered by Creative Biolabs Support.
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Related Signaling Pathways
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
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