Mouse Anti-GAPDH Recombinant Antibody (clone 14C2F11)
CAT#: ZG-0290U
Mouse anti-GAPDH antibody (clone 14C2F11) displayed high specificity against the GAPDH protein of Human, Rat and Rabbit.
Tested Data
Gene Expression
WB
Figure 1 Mouse Anti-GAPDH Antibody (ZG-0290U) in WB
Positive WB detected in: U87 whole cell lysate, PC-3 whole cell lysate, 293 whole cell lysate, U251 whole cell lysate, A549 whole cell lysate, A375 whole cell lysate, MG-63 whole cell lysate, SH-SY5Y whole cell lysate, All lanes GAPDH antibody at 1: 5000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 36 KDa
Observed band size: 36 KDa
Exposure time: 10s
IHC
Figure 2 Mouse Anti-GAPDH Antibody (ZG-0290U) in IHC
IHC image of ZG-0290U diluted at 1:500 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
IF
Figure 3 Mouse Anti-GAPDH Antibody (ZG-0290U) in IF
Immunofluorescence staining of Hela cells with(ZG-0290U)at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Mouse IgG (H+L).
IP
Figure 4 Mouse Anti-GAPDH Antibody (ZG-0290U) in IP
Immunoprecipitating GAPDH in Hela whole cell lysate. Lane 1: Mouse control IgG in Hela whole cell lysate. Lane 2: ZG-0290U (5µl) + Hela whole cell lysate (500µg)
Lane 3: Hela whole cell lysate (10µg)
For western blotting, the blot was detected with ZG-0290U at 1:5000, and a HRP-conjugated Protein G antibody was used as the secondary antibody at 1:2000
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Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/40067/752_B3_1_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear membrane, plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/61280/if_selected.jpg
Normal Tissue
Figure 3 IHC staining of human stomach
Immunohistochemical staining of human stomach, lower shows strong nuclear and cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/40067/ihc_selected.jpg
Normal Tissue
Figure 4 IHC staining of human skin
Immunohistochemical staining of human skin shows strong nuclear positivity in keratinocytes.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/61280/169039_B_8_1_selected.jpg
Normal Tissue
Figure 5 IHC staining of human kidney
Immunohistochemical staining of human kidney shows cytoplasmic and nuclear positivity in cells in tubules.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5197/ihc_selected.jpg
Normal Tissue
Figure 6 IHC staining of human skeletal muscle
Immunohistochemical staining of human skeletal muscle shows strong nuclear positivity in myocytes.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/79968/170623_B_3_6_selected.jpg
Subcellular Location
Figure 7 IF staining of human cell line A549
Immunofluorescent staining of human cell line A549 shows localization to plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/40067/1874_F7_61_blue_red_green.jpg
Normal Tissue
Figure 8 Colon
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/61280/169039_A_8_3.jpg
Normal Tissue
Figure 9 Liver
Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/61280/169039_A_8_4.jpg
Normal Tissue
Figure 10 Kidney
Cells in glomeruli
Staining: Medium
Intensity: Strong
Quantity: <25%
Collecting ducts
Staining: High
Intensity: Strong
Quantity: 75%-25%
Distal tubules
Staining: High
Intensity: Strong
Quantity: 75%-25%
Proximal tubules (cell body)
Staining: High
Intensity: Strong
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/61280/169039_A_7_5.jpg
Normal Tissue
Figure 11 Testis
Cells in seminiferous ducts
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/61280/169039_A_4_6.jpg
Normal Tissue
Figure 12 Lymph node
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/61280/169039_A_7_8.jpg
RNA Expression
Figure 13 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000111640-GAPDH
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Specifications
- Immunogen
- Recombinant Human GAPDH protein (3-335AA)
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- GAPDH
- Species Reactivity
- Human, Rat, Rabbit
- Clone
- 14C2F11
- Applications
- ELISA, WB, IHC, IP, IF, FC
- Conjugate
- Unconjugated
- Related Disease
- Microcephaly 21, Primary, Autosomal Recessive and Aging.
Product Property
- Purification
- Protein G purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol.
- Preservative
- 0.03% Proclin 300
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry, Immunoprecipitation, Immunofluorescence, Flow Cytometry. Specific conditions should be optimized by the end user.
WB:1:5000-1:1600000, IHC:1:50-1:500, IF:1:50-1:200, IP:1µl-2µl, FC:1:100-1:300
Target
- Alternative Names
- G3PD; GAPD; HEL-S-162eP; glyceraldehyde-3-phosphate dehydrogenase; OCAS, p38 component; Oct1 coactivator in S phase, 38 Kd component; aging-associated gene 9 protein; epididymis secretory sperm binding protein Li 162eP; peptidyl-cysteine S-nitrosylase GAPDH
- Gene ID
- 2597
- UniProt ID
- P04406
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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