Rabbit Anti-GRB2 Recombinant Antibody (clone 6E1)
CAT#: ZG-0693J
This product is a rabbit antibody that recognizes GRB2.
Tested Data
Gene Expression
WB
Figure 1 Rabbit Anti-GRB2 Antibody (ZG-0693J) in WB
Western Blot
Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, Mouse Brain whole cell lysate, Rat Brain whole cell lysate
All lanes: GRB2 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 26, 21 kDa
Observed band size: 28 kDa
IHC
Figure 2 Rabbit Anti-GRB2 Antibody (ZG-0693J) in IHC
IHC image of this product diluted at 1:100 and staining in paraffin-embedded human ovarian cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
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Subcellular Location
Figure 1 IF staining of human cell line PC-3
Immunofluorescent staining of human cell line PC-3 shows localization to nucleoplasm & nucleoli.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/30749/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line PC-3
Immunofluorescent staining of human cell line PC-3 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/30750/if_selected.jpg
Normal Tissue
Figure 3 IHC staining of human tonsil
Immunohistochemical staining of human tonsil shows cytoplasmic and nuclear positivity in germinal and non germinal center .
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2589/7330_A_4_8_selected.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2589/7330_A_7_3.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2589/7330_A_8_5.jpg
Normal Tissue
Figure 6 Testis
Leydig cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Spermatogonia cells Staining: Medium Intensity: Moderate
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2589/7330_A_4_6.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2589/7330_A_8_8.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000177885-GRB2
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Specifications
- Immunogen
- A synthesized peptide derived from human GRB2
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human GRB2
- Species Reactivity
- Human, Mouse, Rat
- Clone
- 6E1
- Applications
- ELISA, WB, IHC
- Conjugate
- Uconjugated
- Related Disease
- Cancer
Product Property
- Purification
- Affinity purified
- Format
- Liquid
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry.
WB:1:500-1:5000, IHC:1:50-1:200
Target
- Alternative Names
- ASH; Grb3-3; MST084; NCKAP2; MSTP084; EGFRBP-GRB2; growth factor receptor-bound protein 2; HT027; SH2/SH3 adapter GRB2; abundant SRC homology; epidermal growth factor receptor-binding protein GRB2; epididymis secretory sperm binding protein; growth factor receptor-bound protein 3; protein Ash
- Gene ID
- 2885
- UniProt ID
- P62993
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Related Diseases
Breast Cancer
Colorectal Cancer
Endometrial Cancer
Gastric Cancer
Non-small Cell Lung Cancer
Prostate Cancer
Throid Cancer
Insulin Resistance
Alcoholism
Related Signaling Pathways
ErbB Signaling Pathway
FcεR1 Signaling Pathway
MAPK Signaling Pathway
mTOR Signaling Pathway
PPAR Signaling Pathway
Ras Signaling Pathway
TCR Signaling Pathway
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "GRB2"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1410MZ | Recombinant Mouse Anti-Human GRB2 Antibody (clone S22) | WB | Mouse antibody |
| MRO-0692-CN | Mouse Anti-GRB2 Recombinant Antibody (clone 11-B11) | WB, IHC, IF | Mouse IgG2b |
| FAMAB-1154CQ | Mouse Anti-GRB2 Recombinant Antibody (clone SAIC-19D-1F4) | Immuno-MRM, WB | Mouse IgG1 |
| VS3-QX475 | Mouse Anti-GRB2 Recombinant Antibody (clone 5G3-E7-F11) | WB | Mouse IgG1 |
| VS4-WK202 | Mouse Anti-GRB2 Recombinant Antibody (VS4-WK202) | ELISA, WB | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0235MZ | Chicken Anti-GRB2 Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1379 | Rabbit Anti-GRB2 Recombinant Antibody (clone DS1379AB) | WB, ICC, IF, FC, IP | Rabbit IgG |
| MOR-1502 | Rabbit Anti-GRB2 Recombinant Antibody (clone DS1502AB) | WB, IP, IHC-P, ICC, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY949 | Anti-GRB2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY2906 | Anti-Mouse GRB2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY2905 | Anti-Human GRB2 Immunohistochemistry Kit | IHC |
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