Rabbit Anti-GRB2 Recombinant Antibody (clone 6E1) (CAT#: ZG-0693J)
This product is a rabbit antibody that recognizes GRB2.
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Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
WB
Figure 1 Rabbit Anti-GRB2 Antibody (ZG-0693J) in WB
Western Blot
Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, Mouse Brain whole cell lysate, Rat Brain whole cell lysate
All lanes: GRB2 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 26, 21 kDa
Observed band size: 28 kDa
IHC
Figure 2 Rabbit Anti-GRB2 Antibody (ZG-0693J) in IHC
IHC image of this product diluted at 1:100 and staining in paraffin-embedded human ovarian cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Specifications
- Immunogen
- A synthesized peptide derived from human GRB2
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human GRB2
- Species Reactivity
- Human, Mouse, Rat
- Clone
- 6E1
- Applications
- ELISA, WB, IHC
- Conjugate
- Uconjugated
- Related Disease
- Cancer
Product Property
- Purification
- Affinity purified
- Format
- Liquid
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry.
WB:1:500-1:5000, IHC:1:50-1:200
Target
- Alternative Names
- ASH; Grb3-3; MST084; NCKAP2; MSTP084; EGFRBP-GRB2; growth factor receptor-bound protein 2; HT027; SH2/SH3 adapter GRB2; abundant SRC homology; epidermal growth factor receptor-binding protein GRB2; epididymis secretory sperm binding protein; growth factor receptor-bound protein 3; protein Ash
- Gene ID
- 2885
- UniProt ID
- P62993
Related Resources
Breast Cancer
Colorectal Cancer
Endometrial Cancer
Gastric Cancer
Non-small Cell Lung Cancer
Prostate Cancer
Throid Cancer
Insulin Resistance
Alcoholism
ErbB Signaling Pathway
FcεR1 Signaling Pathway
MAPK Signaling Pathway
mTOR Signaling Pathway
PPAR Signaling Pathway
Ras Signaling Pathway
TCR Signaling Pathway
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0235MZ | Chicken Anti-GRB2 Polyclonal IgY | WB | Chicken antibody |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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