Mouse Anti-GZMB Recombinant Antibody (clone 2G12G7)
CAT#: VS7-0425-WR7
This product is a recombinant mouse IgG2a antibody that binds human GZMB. 2G12G7 recognizes the specific AA domains 21-247 on GZMB. The antibody can be used for IHC assay.
Gene Expression
Normal Tissue
Figure 1 Bone marrow
Hematopoietic cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3418/10586_B_4_4.jpg
Normal Tissue
Figure 2 Lymph node
Non-germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3418/10586_A_7_8.jpg
RNA Expression
Figure 3 RNA cell line category: Cell line enriched (HMC-1)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000100453-GZMB
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Specifications
- Immunogen
- Recombinant human GZMB (AA: 21-247) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG2a
- Specificity
- Human GZMB
- Species Reactivity
- Human
- Clone
- 2G12G7
- Molecule Class
- Enzyme
- Applications
- IHC
- Conjugate
- Unconjugated
- Related Disease
- Cancer; Autoimmune diseases; Infections; Inflammatory diseases
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunohistochemistry-Paraffin: 1:200-1:1000
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- C11; HLP; CCPI; CGL1; CSPB; SECT; CGL-1; CSP-B; CTLA1; CTSGL1
- Gene ID
- 3002
- UniProt ID
- P10144
- Long Name
- Granzyme B
- Cellular Localization
- Lysosome; Secreted
- Post Translation Modifications
- Disulfide bond; Glycoprotein; Zymogen
- Protein Refseq
- NP_001332940.1
- Function
- Abundant protease in the cytosolic granules of cytotoxic T-cells and NK-cells which activates caspase-independent pyroptosis when delivered into the target cell through the immunological synapse (PubMed:1985927, PubMed:3262682, PubMed:3263427).; It cleaves after Asp (PubMed:1985927, PubMed:8258716).; Once delivered into the target cell, acts by catalyzing cleavage of gasdermin-E (GSDME), releasing the pore-forming moiety of GSDME, thereby triggering pyroptosis and target cell death (PubMed:31953257, PubMed:32188940).; Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -9 and -10 (CASP3, CASP9 and CASP10, respectively) to give rise to active enzymes mediating apoptosis (PubMed:9852092).; Cleaves and activates CASP7 in response to bacterial infection, promoting plasma membrane repair (By similarity)
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Related Diseases
Allograft Rejection
Autoimmune Thyroid Disease
Graft-versus-Host Disease
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
Reconstitution Buffer
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
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