Human Anti-HCV Recombinant Antibody (clone B12.F8) (CAT#: FAMAB-0024WJ)

This product is a human antibody hepatitis C virus core. This antibody can be used in applications, such as ELISA, IF, and IHC.


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IF

Figure 1 Detection of Hepatitis C Virus Core protein in BHK-21 cells by indirect immunofluorescence.

Figure 1 Detection of Hepatitis C Virus Core protein in BHK-21 cells by indirect immunofluorescence.

Cells were fixed and incubated with human monoclonal anti-HCV Core antibody B12.F8 (provided by Dr. Mario Mondelli, University of Pavia) and visualized by indirect immunofluorescence (Axiovert 135 fluorescence Microscope, objective 40x). These micrographs show representative fields from the experiments.

Navas, M. C., Stoll-Keller, F., & Pavlovic, J. (2019). Lack of expression of HCV Core Protein in human Monocyte-Derived Dendritic Cells using recombinant Semliki Forest Virus. Acta Biológica Colombiana, 24(3).

WB

Figure 2 Hepatitis C Virus Core protein expression in BHK-21 cells after infection using recombinant SFV.

Figure 2 Hepatitis C Virus Core protein expression in BHK-21 cells after infection using recombinant SFV.

Cells lysates were separated in 10 % SDS-PAGE, then transferred onto a nitrocellulose membrane and immunostained with human antibody anti-HCV Core B12.F8 (provided by Dr. Mario Mondelli, University of Pavia).

Navas, M. C., Stoll-Keller, F., & Pavlovic, J. (2019). Lack of expression of HCV Core Protein in human Monocyte-Derived Dendritic Cells using recombinant Semliki Forest Virus. Acta Biológica Colombiana, 24(3).


Specifications

  • Immunogen
  • HCV protein
  • Host Species
  • Human
  • Derivation
  • Phage display library
  • Type
  • Human IgG
  • Specificity
  • HCV core
  • Species Reactivity
  • HCV
  • Clone
  • B12.F8
  • Applications
  • ELISA, IF, IHC, WB
  • Related Disease
  • HCV infection

Product Property

  • Purity
  • >95% as determined by SDS-PAGE and HPLC analysis
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Buffer
  • PBS
  • Preservative
  • No preservatives
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • The HCV antibody has been reported in applications of Enzyme-linked Immunosorbent Assay, Immunofluorescence, Immunohistochemistry, Western Blot.

Target

  • Alternative Names
  • Hepatitis C virus; HCV

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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