Mouse Anti-IL1RAP Antibody (1F5), CHO Stable Cell Pool
CAT#: VS-1025-QX112
This CHO Stable Cell Pool represents a genetically engineered Chinese Hamster Ovary (CHO) cell population specifically designed for the robust and consistent expression of recombinant 1F5. 1F5 is a Mouse monoclonal antibody that targets IL1RAP. This cell pool was generated through the stable integration of genes encoding the heavy and light chains of 1F5 into the CHO genome, followed by selection and enrichment for high-expressing cells. This cell pool provides rapid production of research-grade antibodies for non-GLP preclinical studies.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol & vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35293/372_B3_2_selected.jpg
Normal Tissue
Figure 2 IHC staining of human lymph node
Immunohistochemical staining of human lymph node shows strong cytoplasmic positivity in subsets of cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35293/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35293/79779_B_7_5.jpg
Normal Tissue
Figure 4 Colon
Peripheral nerve/ganglion
Staining: Not detected
Intensity: Weak
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35293/79779_A_9_3.jpg
Normal Tissue
Figure 5 Kidney
Cells in tubules
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35293/79779_A_7_5.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Leydig cells
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35293/79779_A_6_6.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enriched (SK-MEL-30)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000196083-IL1RAP
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Specifications
- Host Cell
- CHO
- Product Type
- Stable Cell Pool
- Cell Viability
- >90%
- Mycoplasma Detection
- Negative
- Bacteria Detection
- Negative
- Growth Properties
- Suspension
- Culture Medium
- VersaCHO™ Medium (VS-1025-QX582)
- Complete Growth Medium
- VersaCHO™ Medium (VS-1025-QX582), VersaCHO™ Feed Medium (VS-1025-QX583), VersaCHO™ Feed Supplement (VS-1025-QX584)
- Freeze Medium
- 90% VersaCHO™ Medium, 10% DMSO
- Growth Conditions
- 37°C, 5% CO₂
- Size
- Each vial contains at least 1×10^6 cells in 1 mL
- Shipping
- Dry ice
- Storage
- Store in liquid nitrogen immediately upon receipt. Avoid multiple freeze/thaw cycles. Utilize cells within one year of the delivery date.
Antibody Overview
- Host Species
- Mouse
- Antibody Type
- Mouse IgG2a, κ
- Clone
- 1F5
- Target
- IL1RAP
- Species Reactivity
- Human
Target
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Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
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- CAT
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| HPAB-M0109-YC-S(P) | Human Anti-IL1RAP Recombinant Antibody; scFv Fragment (HPAB-M0109-YC-S(P)) | ELISA | Human scFv |
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| HPAB-M0110-YC-S(P) | Human Anti-IL1RAP Recombinant Antibody; scFv Fragment (HPAB-M0110-YC-S(P)) | ELISA | Human scFv |
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| HPAB-M0111-YC-S(P) | Human Anti-IL1RAP Recombinant Antibody; scFv Fragment (HPAB-M0111-YC-S(P)) | ELISA | Human scFv |
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| HPAB-M0110-YC-F(E) | Human Anti-IL1RAP Recombinant Antibody; Fab Fragment (HPAB-M0110-YC-F(E)) | ELISA | Humanized Fab |
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| HPAB-M0111-YC-F(E) | Human Anti-IL1RAP Recombinant Antibody; Fab Fragment (HPAB-M0111-YC-F(E)) | ELISA | Humanized Fab |
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