Rabbit Anti-IL1RAP Recombinant Antibody (clone R05-3B8)
CAT#: VS3-FY2014
The anti-IL-1 receptor accessory protein antibody detected positive in western blot analysis of IL-1RAcP in HL-60, U251, U87-MG lysates. The recombinant monoclonal antibody exhibits high specificity and affinity for human IL1RAP. It is suitable for use in a variety of assays, including WB, IP.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol & vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35293/372_B3_2_selected.jpg
Normal Tissue
Figure 2 IHC staining of human lymph node
Immunohistochemical staining of human lymph node shows strong cytoplasmic positivity in subsets of cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35293/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35293/79779_B_7_5.jpg
Normal Tissue
Figure 4 Colon
Peripheral nerve/ganglion
Staining: Not detected
Intensity: Weak
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35293/79779_A_9_3.jpg
Normal Tissue
Figure 5 Kidney
Cells in tubules
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35293/79779_A_7_5.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Leydig cells
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35293/79779_A_6_6.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enriched (SK-MEL-30)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000196083-IL1RAP
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WB
Figure 1 Anti-IL1RAP Antibody (VS3-FY2014) in WB.
The IL-1RAcP expression in HL-60, U251, U87-MG lysates were detected using the anti-IL-1 Receptor Accessory Protein by western blot assay.
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Specifications
- Immunogen
- Recombinant human IL-1RAcP protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human IL1RAP
- Species Reactivity
- Human
- Clone
- R05-3B8
- Applications
- Western Blot, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 65 kDa, while the observed molecular weight stands at 78 kDa.
- Related Disease
- Autoinflammatory diseases
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunoprecipitation: 1/20
Target
- Alternative Names
- Interleukin 1 Receptor Accessory Protein
- Gene ID
- 3556
- UniProt ID
- Q9NPH3
- Sequence Similarities
- Belongs to the interleukin-1 receptor family.
- Cellular Localization
- Cell membrane
- Post Translation Modifications
- Glycosylation at Asn57, Asn107, Asn111, Asn118, Asn196, Asn209, and Asn299 (NX_Q9NPH3 [NX_Q9NPH3-1])
Modification sites at PhosphoSitePlus (Q9NPH3)
Glycosylation from GlyConnect IL1AP_HUMAN (1425)
Glycosylation from GlyGen (Q9NPH3) 9 sites, 13 N-linked glycans (3 sites)
- Protein Refseq
- XP_016861837.1; XP_047304037.1; XP_047304038.1; XP_047304039.1; XP_047304040.1
- Function
- The cellular response is modulated through a non-signaling association with the membrane IL1R2 decoy receptor. Coreceptor for IL1RL1 in the IL-33 signaling system. Can bidirectionally induce pre- and postsynaptic differentiation of neurons by trans-synaptically binding to PTPRD (By similarity).
May play a role in IL1B-mediated costimulation of IFNG production from T-helper 1 (Th1) cells (Probable).
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
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