This product is a recombinant Human antibody that can recognize IL2.
Figure 1 Anti-IL2 Recombinant Antibody (PABC-506) in ELISA
ELISA analysis of PABC-506 was performed by coating with human IL2 protein (His tag). Then blocked with BSA and incubated with PABC-506. The HRP-conjugated goat anti-IgG was used as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Figure 2 Anti-IL2 Recombinant Antibody (PABC-506) in WB
Western blot analysis of PABC-506 was performed by loading human IL2 protein onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with PABC-506 and a HRP goat anti-IgG (H+L) was used as the secondary antibody (1: 3000). Chemiluminescent detection was performed.
Figure 3 Anti-IL2 Recombinant Antibody (PABC-506) in DB
Dot Blot analysis of PABC-506 was performed by coating with human IL2 protein (His tag).
PABC-506 incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-IgG (H+L) secondary antibody (1: 3000)
NC: Negative control, PC: Positive control
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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PABL-609 | Mouse Anti-Il2 Recombinant Antibody (clone JES6-1) | FC, FuncS, IHC, ELISA | Mouse IgG |
PABL-610 | Mouse Anti-Il2 Recombinant Antibody (clone S4B6) | ELISA, FC, FuncS | Mouse IgG |
FAMAB-0179YC | Human Anti-IL2 Recombinant Antibody (clone C5) | ELISA, WB | Human IgG |
VS-0723-WK216 | Rabbit Anti-IL2 Recombinant Antibody (VS-0723-WK216) | ELISA, FC | Rabbit IgG |
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To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# PABC-506, RRID: AB_3111595)
For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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