Human Anti-IL2 Recombinant Antibody (PABC-506) (CAT#: PABC-506)

Figure 1 Antibody F5111.2 inhibits upregulation of IL-2-induced proteins in CD8+ T cells but not Tregs in vitro.

Dose response of CD25 and FoxP3 expression on Treg, Tconv and CD8 cells following expansion of human PBMCs in the presence of hIL-2 (5 ng/ml)–F5111.2 complexes for five days. Representative results from one of five experiments are shown.

Trotta, E., Bessette, P. H., Silveria, S. L., Ely, L. K., Jude, K. M., Le, D. T., ... & Garcia, K. C. (2018). A human anti-IL-2 antibody that potentiates regulatory T cells by a structure-based mechanism. Nature medicine, 24(7), 1005.

Figure 1 Anti-IL2 Recombinant Antibody (PABC-506) in ELISA

ELISA analysis of PABC-506 was performed by coating with human IL2 protein (His tag). Then blocked with BSA and incubated with PABC-506. The HRP-conjugated goat anti-IgG was used as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.

Figure 2 Anti-IL2 Recombinant Antibody (PABC-506) in WB

Western blot analysis of PABC-506 was performed by loading human IL2 protein onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with PABC-506 and a HRP goat anti-IgG (H+L) was used as the secondary antibody (1: 3000). Chemiluminescent detection was performed.

Figure 3 Anti-IL2 Recombinant Antibody (PABC-506) in DB

Dot Blot analysis of PABC-506 was performed by coating with human IL2 protein (His tag).
PABC-506 incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-IgG (H+L) secondary antibody (1: 3000)
NC: Negative control, PC: Positive control