Rabbit Anti-IL3RA Antibody (VS-0924-YC48) - Cancer Stem Cell Marker

Name: Rabbit Anti-IL3RA Antibody (VS-0924-YC48) - Cancer Stem Cell Marker
SKU: VS-0924-YC48
Rating: 5 (1 reviews)

CAT#: VS-0924-YC48

This product is an anti-IL3RA antibody (VS-0924-YC48) that targets the human IL3RA protein, a marker of cancer stem cells (CSCs). It can be utilized for detecting IL3RA in CSC tissues or cells by immunohistochemistry at dilutions of 1/50 to 1/200.

Gene Expression

Normal Tissue Figure 1 IHC staining of human small intestine

Immunohistochemical staining of human small intestine shows cytoplasmic and membranous positivity in glandular cells.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3539/ihc_selected.jpg

Normal Tissue Figure 2 Cerebral cortex

Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Glial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Neuronal cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3539/11801_B_8_5.jpg

Normal Tissue Figure 3 Colon

Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3539/11801_A_9_3.jpg

Normal Tissue Figure 4 Kidney

Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3539/11801_A_8_5.jpg

Normal Tissue Figure 5 Testis

Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3539/11801_A_5_6.jpg

Normal Tissue Figure 6 Lymph node

Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3539/11801_A_9_8.jpg

RNA Expression Figure 7 RNA cell line category: Cell line enhanced (HDLM-2, NB-4, RPMI-8226)

Cell lines ordered by descending RNA expression order

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000185291-IL3RA

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Specifications

Host Species
Rabbit

Type
Rabbit IgG

Specificity
Human IL3RA

Species Reactivity
Human

Applications
IHC

Product Property

Clonality
Polyclonal

Purification
Affinity purified using the PrEST antigen as affinity ligand

Concentration
0.05 mg/mL

Buffer
PBS, pH 7.2, with 40% glycerol

Preservative
0.02% Sodium azide

Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

Application Notes
Immunohistochemistry (IHC): 1/50-1/200

Target

Alternative Names
CD123

Gene ID
3563

UniProt ID
P26951

Long Name
Interleukin 3 receptor, alpha (low affinity)

Cellular Localization
#Membrane

Post Translation Modifications
#Disulfide bond
#Glycoprotein
#Ubl conjugation

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Downloadable Resources

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Datasheet

Datasheet - VS-0924-YC48.pdf

MSDS

MSDS - VS-0924-YC48.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

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Protocol & Troubleshooting

Immunohistochemistry (IHC)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

Isotype Control

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Product Name

Secondary Antibody

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Product Name

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