Rabbit Anti-JUN Recombinant Antibody (clone 4A11) (CAT#: ZG-0782J)
This product is a rabbit antibody that recognizes JUN.
Specific Inquiry
Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
WB
Figure 1 Rabbit Anti-JUN Antibody (ZG-0782J) in WB
Western Blot
Positive WB detected in: Hela whole cell lysate, A549 whole cell lysate(treated with Calyculin A or EGF)
All lanes: Phospho-JUN antibody at 0.95μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 48 KDa
Observed band size: 48 KDa
IHC
Figure 2 Rabbit Anti-JUN Antibody (ZG-0782J) in IHC
IHC image of this product diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IF
Figure 3 Rabbit Anti-JUN Antibody (ZG-0782J) in IF
Immunofluorescence staining of A549 cells with this product at 1:100,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L).
Specifications
- Immunogen
- A synthesized peptide derived from human Phospho-JUN (S63)
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human JUN
- Species Reactivity
- Human
- Clone
- 4A11
- Applications
- ELISA, WB, IHC, IF
- Conjugate
- Uconjugated
- Related Disease
- Tumor
Product Property
- Purification
- Affinity purified
- Format
- Liquid
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry, Immunofluorescence.
WB:1:500-1:5000, IHC:1:50-1:200, IF:1:20-1:200
Target
- Alternative Names
- AP1; p39; AP-1; cJUN; c-Jun; transcription factor AP-1; Jun activation domain binding protein; activator protein 1; enhancer-binding protein AP1; jun oncogene; proto-oncogene c-Jun; proto-oncogene cJun; v-jun avian sarcoma virus 17 oncogene homolog; v-jun sarcoma virus 17 oncogene homolog
- Gene ID
- 3725
- UniProt ID
- P05412
Related Resources
BCR Signaling Pathway
cAMP Signaling Pathway
NLR Signaling Pathway
TNF Signaling Pathway
Bladder Cancer
Endometrial Cancer
Non-small Cell Lung Cancer
Amphetamine Addiction
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "JUN"
Select a product category from the dropdown menu below to view related products.
Please select product type
IgG Antibody Products
Chicken IgY Antibody Products
Rabbit Monoclonal Antibody Products
Antibody Magnetic Beads
Non-Human Primate (NHP) Antibody Products
IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1499z | Mouse Anti-JUN Recombinant Antibody (clone 23G9) | WB, ELISA, ICC, IF, IHC | Mouse IgG1 |
| MRO-0365-CN | Recombinant Rabbit Anti-JUN Monoclonal Antibody (CBACN-148) | WB, IF, IHC, IP | Rabbit IgG |
| ZG-0293F | Mouse Anti-JUN Recombinant Antibody (ZG-0293F) | WB | Mouse IgG |
| VS7-0425-WR171 | Mouse Anti-JUN Monoclonal Antibody (VS7-0425-WR171) | WB, IHC-P, IF, ICC, ELISA | Mouse IgG |
| VS7-0425-WR172 | Mouse Anti-JUN Monoclonal Antibody (VS7-0425-WR172) | WB, IHC-P, IF, ICC, FC, ELISA | Mouse IgG |
Customer Reviews and Q&As
There are currently no Customer reviews or questions for ZG-0782J. Click the button above to contact us or submit your feedback about this product.
Popular products with customers
Application: IF, IP, Neut, FuncS, ELISA, FC, WB
Application: Neut, ELISA, IF, IP, FuncS, FC, IHC
![Figure 2 Anti-Human CD19 Recombinant Antibody Fab Fragment [TAB-1611CL-F(E)] in HPLC](https://img.creativebiolabs.net/productimages/COA-TAB-1611CL-F(E)-2.png)
Application: Depletion, FuncS
Application: WB, IF, FuncS
-YJ5.png)
Application: WB, ELISA, FC, IHC, IP
Application: ELISA, FC, WB, FuncS
Application: Neut, ELISA, FuncS
Application: ELISA, Vaccine, FuncS
For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
