Rabbit Anti-MET (pY1349) Recombinant Antibody (clone R05-3D6)
CAT#: VS3-WK2033
This product is a rabbit recombinant antibody that is specific to phosphorylated MET. The target of this antibody is phospho-MET (Tyr1349). The clone R05-3D6 can be used in WB analysis at a dilution of 1/500-1/1000 and IP analysis at a dilution of 1/20. The molecular weight of MET (pTyr1349) observed in WB experiments using this antibody is 140-170 kDa.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/55607/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5282/if_selected.jpg
Subcellular Location
Figure 3 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18577/if_selected.jpg
Normal Tissue
Figure 4 Kidney
Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5282/15802_A_7_5.jpg
Normal Tissue
Figure 5 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5282/15802_A_6_6.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (A-431, CAPAN-2)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000105976-MET
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WB
Figure 1 Rabbit Anti-MET (pTyr1349) Recombinant Antibody (clone R05-3D6) in WB.
Western blot analysis of phospho-Met (c-Met) (Tyr1349) in Hela, A549 lysates using anti-MET (pTyr1349) antibody, Cat# VS3-WK2033.
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Specifications
- Immunogen
- A synthetic phosphopeptide corresponding to residues Tyr1349 of human Met
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Phospho-Tyr1349 of human MET
- Species Reactivity
- Human
- Clone
- R05-3D6
- Applications
- WB, IP
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IP: 1/20
Target
- Alternative Names
- MET Proto-Oncogene, Receptor Tyrosine Kinase; Hepatocyte Growth Factor Receptor; Tyrosine-Protein Kinase Met; Scatter Factor Receptor; Proto-Oncogene C-Met; HGF/SF Receptor; HGF Receptor; SF Receptor; EC 2.7.10.1
- Gene ID
- 4233
- UniProt ID
- P08581
- Long Name
- MET Proto-Oncogene, Receptor Tyrosine Kinase
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Related Diseases
EGFR Tyrosine Kinase Inhibitor Resistance
Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-002MZ | Anti-Human MET Recombinant Antibody (TAB-002MZ) | IHC, PET imaging | Chimeric antibody (mouse/human) |
| TAB-002MZ-S(P) | Anti-Human MET Recombinant Antibody scFv Fragment (TAB-002MZ-S(P)) | IHC, PET imaging | Chimeric antibody (mouse/human) |
| TAB-002MZ-F(E) | Anti-Human MET Recombinant Antibody Fab Fragment (TAB-002MZ-F(E)) | IHC, PET imaging | Chimeric antibody (mouse/human) |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0071-YC-S(P) | Mouse Anti-MET Recombinant Antibody; scFv Fragment (HPAB-0071-YC-S(P)) | ELISA | Mouse scFv |
| HPAB-0072-YC-S(P) | Human Anti-MET Recombinant Antibody; scFv Fragment (HPAB-0072-YC-S(P)) | ELISA | Humanized scFv |
| HPAB-0073-YC-S(P) | Human Anti-MET Recombinant Antibody; scFv Fragment (HPAB-0073-YC-S(P)) | ELISA | Human scFv |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-1298LY | Human Anti-MET Recombinant Antibody (HPAB-1298LY) | ELISA, IP | Human IgG2 |
| HPAB-1299LY | Human Anti-MET Recombinant Antibody (HPAB-1299LY) | ELISA, IP | Human IgG2 |
| HPAB-1300LY | Human Anti-MET Recombinant Antibody (HPAB-1300LY) | ELISA, IP | Human IgG2 |
| HPAB-1301LY | Human Anti-MET Recombinant Antibody (HPAB-1301LY) | ELISA, IP | Human IgG2 |
| HPAB-1302LY | Human Anti-MET Recombinant Antibody (HPAB-1302LY) | ELISA, IP | Human IgG2 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-1298LY-F(E) | Human Anti-MET Recombinant Antibody; Fab Fragment (HPAB-1298LY-F(E)) | ELISA, IP | Human Fab |
| HPAB-1299LY-F(E) | Human Anti-MET Recombinant Antibody; Fab Fragment (HPAB-1299LY-F(E)) | ELISA, IP | Human Fab |
| HPAB-1300LY-F(E) | Human Anti-MET Recombinant Antibody; Fab Fragment (HPAB-1300LY-F(E)) | ELISA, IP | Human Fab |
| HPAB-1301LY-F(E) | Human Anti-MET Recombinant Antibody; Fab Fragment (HPAB-1301LY-F(E)) | ELISA, IP | Human Fab |
| HPAB-1302LY-F(E) | Human Anti-MET Recombinant Antibody; Fab Fragment (HPAB-1302LY-F(E)) | ELISA, IP | Human Fab |
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