Rabbit Anti-MMP9 Recombinant Antibody (clone R08-4E2) (CAT#: VS3-XY3432)
The rabbit recombinant antibody specifically recognizes MMP9 (Matrix metallopeptidase 9), which plays a role in bone osteoclastic resorption. It specifically reacts with human and mouse MMP9. It has been tested using Western Blot, Immunohistochemistry-Paraffin, Flow cytometry, Immunocytochemistry or Immunofluorescence.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1238/if_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1238/if_selected.jpg
Figure 2 IHC staining of human bone marrow
(Immunohistochemical staining of human bone marrow shows strong nuclear and cytoplasmic positivity in bone marrow poietic cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/348/ihc_selected.jpg
(Immunohistochemical staining of human bone marrow shows strong nuclear and cytoplasmic positivity in bone marrow poietic cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/348/ihc_selected.jpg
Figure 3 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1238/30_H1_1_blue_red_green.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1238/30_H1_1_blue_red_green.jpg
Figure 4 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1238/31_H1_1_blue_red_green.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1238/31_H1_1_blue_red_green.jpg
Figure 5 Testis
(Preleptotene spermatocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Spermatogonia cells
Staining: Medium
Intensity: Moderate
Quantity:>75%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/63909/152375_A_6_6.jpg
(Preleptotene spermatocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Spermatogonia cells
Staining: Medium
Intensity: Moderate
Quantity:>75%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/63909/152375_A_6_6.jpg
Figure 6 Lymph node
(Non-germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/63909/152375_A_8_8.jpg
(Non-germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/63909/152375_A_8_8.jpg
Figure 7 RNA cell line category: Cell line enriched (NB-4)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000100985-MMP9
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000100985-MMP9
Figure 7 RNA cell line category: Cell line enriched (NB-4)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000100985-MMP9
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000100985-MMP9
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Rat MMP9
- Species Reactivity
- Human, Rat
- Clone
- R08-4E2
- Applications
- WB, IHC-P, ICC, IF, FC
- Related Disease
- Metaphyseal Anadysplasia 2; Metaphyseal Anadysplasia
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IHC: 1/50-1/100
IF: 1/50-1/200
FC: 1/50-1/100
Target
- Alternative Names
- GELB; CLG4B; MMP-9; MANDP2
- Gene ID
- 4318
- UniProt ID
- P14780
- Long Name
- Matrix metallopeptidase 9
- Cellular Localization
- Secreted
- Post Translation Modifications
- Processing of the precursor yields different active forms of 64, 67 and 82 kDa.
Sequentially processing by MMP3 yields the 82 kDa matrix metalloproteinase-9.
(P14780-MMP9_HUMAN)
N- and O-glycosylated. (P14780-MMP9_HUMAN)
Glycosylation at Asn38, Asn120, and Asn127 (NX_P14780 [NX_P14780-1])
Modification sites at PhosphoSitePlus (P14780)
Glycosylation from GlyConnect MMP9_HUMAN (6)
Glycosylation from GlyGen (P14780) 4 sites, 1 N-linked glycan (1 site), 29 O-linked glycans (1 site)
- Protein Refseq
- NP_004985.2
- Function
- Matrix metalloproteinase that plays an essential role in local proteolysis of the extracellular matrix and in leukocyte migration (PubMed:2551898, 1480034, 12879005).
Could play a role in bone osteoclastic resorption (By similarity).
Cleaves KiSS1 at a Gly-|-Leu bond (PubMed:12879005).
Cleaves NINJ1 to generate the Secreted ninjurin-1 form (PubMed:32883094).
Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments (PubMed:1480034).
Degrades fibronectin but not laminin or Pz-peptide.
Recommended Products
Isotype Control
Recommended Dilution Buffer
Secondary Antibody
Related Resources
TNF Signaling Pathway
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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ScFv-Fc Chimera Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-1048 | Recombinant Mouse Anti-Human MMP9 Antibody Fab Fragment | LMNX, ELISA, WB, IF | Fab |
| PFBC-174 | Mouse Anti-MMP9 Recombinant Antibody Fab Fragment (PFBC-174) | ELISA, Neut | Mouse Fab |
| HPAB-0199-FY-F(E) | Human Anti-MMP9 Recombinant Antibody (clone DX-2802); Fab Fragment | ELISA, FC | Human Fab, λ |
| HPAB-0200-FY-F(E) | Human Anti-MMP9 Recombinant Antibody (clone M0279-A03); Fab Fragment | ELISA, FC | Human Fab |
| HPAB-M0311-YC-F(E) | Mouse Anti-MMP9 Recombinant Antibody (clone AB0046); Fab Fragment | ELISA | Mouse Fab |
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