Rabbit Anti-MMP9 Recombinant Antibody (clone R08-4E2) (CAT#: VS3-XY3432)
The rabbit recombinant antibody specifically recognizes MMP9 (Matrix metallopeptidase 9), which plays a role in bone osteoclastic resorption. It specifically reacts with human and mouse MMP9. It has been tested using Western Blot, Immunohistochemistry-Paraffin, Flow cytometry, Immunocytochemistry or Immunofluorescence.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1238/if_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1238/if_selected.jpg
Figure 2 IHC staining of human bone marrow
(Immunohistochemical staining of human bone marrow shows strong nuclear and cytoplasmic positivity in bone marrow poietic cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/348/ihc_selected.jpg
(Immunohistochemical staining of human bone marrow shows strong nuclear and cytoplasmic positivity in bone marrow poietic cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/348/ihc_selected.jpg
Figure 3 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1238/30_H1_1_blue_red_green.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1238/30_H1_1_blue_red_green.jpg
Figure 4 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1238/31_H1_1_blue_red_green.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1238/31_H1_1_blue_red_green.jpg
Figure 5 Testis
(Preleptotene spermatocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Spermatogonia cells
Staining: Medium
Intensity: Moderate
Quantity:>75%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/63909/152375_A_6_6.jpg
(Preleptotene spermatocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Spermatogonia cells
Staining: Medium
Intensity: Moderate
Quantity:>75%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/63909/152375_A_6_6.jpg
Figure 6 Lymph node
(Non-germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/63909/152375_A_8_8.jpg
(Non-germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/63909/152375_A_8_8.jpg
Figure 7 RNA cell line category: Cell line enriched (NB-4)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000100985-MMP9
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000100985-MMP9
Figure 7 RNA cell line category: Cell line enriched (NB-4)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000100985-MMP9
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000100985-MMP9
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Rat MMP9
- Species Reactivity
- Human, Rat
- Clone
- R08-4E2
- Applications
- WB, IHC-P, ICC, IF, FC
- Related Disease
- Metaphyseal Anadysplasia 2; Metaphyseal Anadysplasia
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IHC: 1/50-1/100
IF: 1/50-1/200
FC: 1/50-1/100
Target
- Alternative Names
- GELB; CLG4B; MMP-9; MANDP2
- Gene ID
- 4318
- UniProt ID
- P14780
- Long Name
- Matrix metallopeptidase 9
- Cellular Localization
- Secreted
- Post Translation Modifications
- Processing of the precursor yields different active forms of 64, 67 and 82 kDa.
Sequentially processing by MMP3 yields the 82 kDa matrix metalloproteinase-9.
(P14780-MMP9_HUMAN)
N- and O-glycosylated. (P14780-MMP9_HUMAN)
Glycosylation at Asn38, Asn120, and Asn127 (NX_P14780 [NX_P14780-1])
Modification sites at PhosphoSitePlus (P14780)
Glycosylation from GlyConnect MMP9_HUMAN (6)
Glycosylation from GlyGen (P14780) 4 sites, 1 N-linked glycan (1 site), 29 O-linked glycans (1 site)
- Protein Refseq
- NP_004985.2
- Function
- Matrix metalloproteinase that plays an essential role in local proteolysis of the extracellular matrix and in leukocyte migration (PubMed:2551898, 1480034, 12879005).
Could play a role in bone osteoclastic resorption (By similarity).
Cleaves KiSS1 at a Gly-|-Leu bond (PubMed:12879005).
Cleaves NINJ1 to generate the Secreted ninjurin-1 form (PubMed:32883094).
Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments (PubMed:1480034).
Degrades fibronectin but not laminin or Pz-peptide.
Recommended Products
Isotype Control
Recommended Dilution Buffer
Secondary Antibody
Related Resources
TNF Signaling Pathway
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "MMP9"
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-1048 | Recombinant Mouse Anti-Human MMP9 Antibody Fab Fragment | LMNX, ELISA, WB, IF | Fab |
| HPAB-0027-YC-F(E) | Mouse Anti-MMP9 Recombinant Antibody; Fab Fragment (HPAB-0027-YC-F(E)) | FC | Mouse Fab |
| HPAB-0028-YC-F(E) | Human Anti-MMP9 Recombinant Antibody (clone G03-VH-GL-V1/ G06-VL-GL1); Fab Fragment | Neut | Human Fab |
| HPAB-0199-FY-F(E) | Human Anti-MMP9 Recombinant Antibody (clone DX-2802); Fab Fragment | ELISA, FC | Human Fab, λ |
| HPAB-0200-FY-F(E) | Human Anti-MMP9 Recombinant Antibody (clone M0279-A03); Fab Fragment | ELISA, FC | Human Fab |
Humanized Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-533CL | Anti-Human MMP9 Recombinant Antibody (GS-5745) | WB, Inhib | Antibody |
Human Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0824CL | Human Anti-MMP9 Recombinant Antibody (TAB-0824CL) | ELISA | Human IgG4 |
| TAB-0825CL | Human Anti-MMP9 Recombinant Antibody (TAB-0825CL) | ELISA | Human IgG4 |
| TAB-0824CL-S(P) | Human Anti-MMP9 Recombinant Antibody; scFv Fragment (TAB-0824CL-S(P)) | ELISA | Human scFv |
| TAB-0825CL-S(P) | Human Anti-MMP9 Recombinant Antibody; scFv Fragment (TAB-0825CL-S(P)) | ELISA | Human scFv |
| TAB-0824CL-F(E) | Human Anti-MMP9 Recombinant Antibody; Fab Fragment (TAB-0824CL-F(E)) | ELISA | Human Fab |
Mouse Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0826CL | Mouse Anti-MMP9 Recombinant Antibody (TAB-0826CL) | Inhib, ELISA, IHC | Mouse IgG |
| TAB-0827CL | Mouse Anti-MMP9 Recombinant Antibody (TAB-0827CL) | ELISA | Mouse IgG |
| TAB-0826CL-S(P) | Mouse Anti-MMP9 Recombinant Antibody; scFv Fragment (TAB-0826CL-S(P)) | ELISA | Mouse scFv |
| TAB-0827CL-S(P) | Mouse Anti-MMP9 Recombinant Antibody; scFv Fragment (TAB-0827CL-S(P)) | ELISA | Mouse scFv |
| TAB-0826CL-F(E) | Mouse Anti-MMP9 Recombinant Antibody; Fab Fragment (TAB-0826CL-F(E)) | ELISA | Mouse Fab |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBC-174 | Mouse Anti-MMP9 Recombinant Antibody (clone SDS3); scFv Fragment | ELISA, Neut | Mouse scFv |
| HPAB-0027-YC-S(P) | Mouse Anti-MMP9 Recombinant Antibody; scFv Fragment (HPAB-0027-YC-S(P)) | FC | Mouse scFv |
| HPAB-0028-YC-S(P) | Human Anti-MMP9 Recombinant Antibody (clone G03-VH-GL-V1/ G06-VL-GL1); scFv Fragment | Neut | Human scFv |
| HPAB-0199-FY-S(P) | Human Anti-MMP9 Recombinant Antibody (clone DX-2802); scFv Fragment | ELISA, FC | Human scFv |
| HPAB-M0311-YC-S(P) | Mouse Anti-MMP9 Recombinant Antibody (clone AB0046); scFv Fragment | ELISA | Mouse scFv |
Chicken IgY Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0717MZ | Chicken Anti-MMP9 Polyclonal IgY | WB | Chicken antibody |
Blocking Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-1748CQ | Recombinant Mouse Anti-MMP9 Antibody (VMC204) | Inhib, IHC-Fr, IP, IHC-P, WB, IHC, ICC | IgG1 |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2263 | Hi-Affi™ Recombinant Rabbit Anti-MMP9 Monoclonal Antibody (DS2263AB) | FC, IHC-P, WB | IgG |
| MOR-4331 | Hi-Affi™ Recombinant Rabbit Anti-MMP-9 Monoclonal Antibody (SI420DS) | ELISA | IgG |
| MOR-4332 | Hi-Affi™ Recombinant Rabbit Anti-MMP-9 Monoclonal Antibody (SI421DS) | ELISA | IgG |
Chimeric Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-002ML | Anti-Human MMP9 Recombinant Antibody (Andecaliximab) | ELISA, IHC, FC, IP, IF, FuncS | IgG4, κ |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0027-YC | Mouse Anti-MMP9 Recombinant Antibody (HPAB-0027-YC) | FC | Mouse IgG2b, κ |
| HPAB-0028-YC | Human Anti-MMP9 Recombinant Antibody (clone G03-VH-GL-V1/ G06-VL-GL1) | Neut | Human IgG4 |
| HPAB-0513LY | Mouse Anti-MMP9 Recombinant Antibody (clone M4) | FC | Mouse IgG |
| HPAB-0514LY | Mouse Anti-MMP9 Recombinant Antibody (clone M12) | FC | Mouse IgG |
| MRO-1014-CN | Recombinant Rabbit Anti-MMP9 Monoclonal Antibody (JA80-73) | WB, IF, IHC, FC | Rabbit IgG |
ADCC Enhanced Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-002ML | Afuco™ Anti-MMP9 ADCC Recombinant Antibody (Andecaliximab), ADCC Enhanced | ELISA, IHC, FC, IP, IF, FuncS | ADCC enhanced antibody |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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