Mouse Anti-mTOR Recombinant Antibody (clone 8H5A5)
CAT#: VS7-0425-WR434
This product is a mouse IgG2a antibody that binds to human mTOR. The antibody 8H5A5 recognizes specific amino acid domain of human mTOR (AA: 2311-2529). It can be used for IHC assay.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line HEK 293
Immunofluorescent staining of human cell line HEK 293 shows localization to vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/71227/1564_C3_1_selected.jpg
Normal Tissue
Figure 2 IHC staining of human testis
Immunohistochemical staining of human testis shows cytoplasmic positivity in cells in seminiferous ducts and Leydig cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/69425/153895_A_6_6_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Glial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/69425/153895_B_9_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/69425/153895_A_9_3.jpg
Normal Tissue
Figure 5 Liver
Hepatocytes Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/69425/153895_A_8_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/69425/153895_A_7_5.jpg
Normal Tissue
Figure 7 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/69425/153895_A_4_6.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000198793-MTOR
❮
❯
Specifications
- Immunogen
- Recombinant human mTOR (AA: 2311-2529) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG2a
- Specificity
- Human mTOR
- Species Reactivity
- Human
- Clone
- 8H5A5
- Molecule Class
- Serine/Threonine Kinase
- Applications
- IHC
- Conjugate
- Unconjugated
- Related Disease
- Smith-Kingsmore syndrome (SKS); Focal cortical dysplasia 2 (FCORD2)
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunohistochemistry-Paraffin: 1:200 - 1:1000
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- MTOR; Rapamycin Associated Protein FRAP2; Rapamycin And FKBP12 Target 1; DJ576K7.1 (FK506 Binding Protein 12-Rapamycin Associated Protein 1); FRAP1; MTOR; FRAP; FKBP12-Rapamycin Complex-Associated Protein 1; Rapamycin Target Protein 1; FKBP-Rapamycin Asso
- Gene ID
- 2475
- UniProt ID
- P42345
- Long Name
- Mechanistic Target Of Rapamycin Kinase
- Cellular Localization
- Cell membrane; Cytoplasm; Cytoplasmic vesicle; Endoplasmic reticulum; Golgi apparatus; Lysosome; Membrane; Microsome; Mitochondrion; Mitochondrion outer membrane; Nucleus
- Post Translation Modifications
- Acetylation; Isopeptide bond; Phosphoprotein; Ubl conjugation
- Protein Refseq
- NP_001373429.1
- Function
- Kinase; Serine/threonine-protein kinase; Transferase
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for VS7-0425-WR434. Click the button above to contact us or submit your feedback about this product.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Related Signaling Pathways
BCR Signaling Pathway
ErbB Signaling Pathway
Insulin Signaling Pathway
mTOR Signaling Pathway
PI3K-Akt Signaling Pathway
Related Diseases
Breast Cancer
Colorectal Cancer
EGFR Tyrosine Kinase Inhibitor Resistance
Gastric Cancer
Non-small Cell Lung Cancer
Hepatocellular Carcinoma
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
Reconstitution Buffer
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
See other products for "MTOR"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2318 | Hi-Affi™ Recombinant Rabbit Anti-MTOR Monoclonal Antibody (DS2318AB) | ICC, IF, IHC-P, WB | IgG |
| MOR-4652 | Hi-Affi™ Recombinant Rabbit Anti-MTOR Monoclonal Antibody (TH165DS) | WB, IF, ICC, FC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-2048-CN | Rabbit Anti-MTOR Polyclonal Antibody (MRO-2048-CN) | IF, IHC, WB | Rabbit IgG |
| ZG-0473U | Rabbit Anti-Phospho-MTOR (S2481) Recombinant Antibody (clone 3H11) | ELISA, WB, IF | Rabbit IgG |
| ZG-0474U | Rabbit Anti-Phospho-MTOR (S2448) Recombinant Antibody (clone 1G6) | ELISA, WB, IHC, IF | Rabbit IgG |
| VS3-FY973 | Recombinant Rabbit Anti-MTOR (phospho Ser2448) Antibody (clone R08-7G7) | WB, IHC-P | Rabbit IgG |
| VS3-WK2060 | Rabbit Anti-MTOR (pS2448) Recombinant Antibody (clone R13-9D-7) | WB, IHC-P | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1390 | Anti-MTOR Immunohistochemistry Kit | IHC | |
| VS-0525-XY4569 | Anti-Mouse MTOR Immunohistochemistry Kit | IHC | |
| VS-0525-XY4568 | Anti-Human MTOR Immunohistochemistry Kit | IHC |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: IP, IF, FuncS, FC, Neut, ELISA, ICC
Application: FuncS, IF, Neut, ELISA, FC, IP, IHC
Application: Neut, ELISA, IF, IP, FuncS, FC, ICC
Application: FC, IP, ELISA, Neut, FuncS, IF, WB
Application: WB, FC, IP, ELISA, Neut, FuncS, IF
Application: ELISA, FC, IP, FuncS, IF, Neut, WB
Application: ELISA, SPR, Inhib, FuncS
-3.png)
Application: ELISA, FC, Block, SPR
Application: ELISA, WB, FC, IHC, IP
Application: Neut, ELISA, IF, IP, FuncS, FC
Application: ELISA, FuncS
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
