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    Mouse Anti-mTOR Recombinant Antibody (clone 8H5A5) (CAT#: VS7-0425-WR434)

    This product is a mouse IgG2a antibody that binds to human mTOR. The antibody 8H5A5 recognizes specific amino acid domain of human mTOR (AA: 2311-2529). It can be used for IHC assay.

    Specific Inquiry
    • Size:

    • Conjugation:

    • Endotoxin:

    • Purity:

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    • Fc Engineering:

    • Modalities:

    Custom Production

    We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

    Sequences:
    Isotype :
    Purity :
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    Other Requirements:
    We require custom production
    • Gene Expression
    • Datasheet
    • MSDS
    • COA
    Subcellular Location
    Figure 1 IF staining of human cell line HEK 293
    Figure 1 IF staining of human cell line HEK 293
    (Immunofluorescent staining of human cell line HEK 293 shows localization to vesicles.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/71227/1564_C3_1_selected.jpg
    Normal Tissue
    Figure 2 IHC staining of human testis
    Figure 2 IHC staining of human testis
    (Immunohistochemical staining of human testis shows cytoplasmic positivity in cells in seminiferous ducts and Leydig cells.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/69425/153895_A_6_6_selected.jpg
    Figure 3 Cerebral cortex
    Figure 3 Cerebral cortex
    (Glial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/69425/153895_B_9_5.jpg
    Figure 4 Colon
    Figure 4 Colon
    (Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/69425/153895_A_9_3.jpg
    Figure 5 Liver
    Figure 5 Liver
    (Hepatocytes Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/69425/153895_A_8_4.jpg
    Figure 6 Kidney
    Figure 6 Kidney
    (Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/69425/153895_A_7_5.jpg
    Figure 7 Testis
    Figure 7 Testis
    (Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/69425/153895_A_4_6.jpg
    RNA Expression
    Figure 8 RNA cell line category: Low cell line specificity
    Figure 8 RNA cell line category: Low cell line specificity
    (Cell lines ordered by descending RNA expression order.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000198793-MTOR

    Specifications

    • Immunogen
    • Recombinant human mTOR (AA: 2311-2529) fragment expressed in E. Coli.
    • Host Species
    • Mouse
    • Type
    • Mouse IgG2a
    • Specificity
    • Human mTOR
    • Species Reactivity
    • Human
    • Clone
    • 8H5A5
    • Molecule Class
    • Serine/Threonine Kinase
    • Applications
    • IHC
    • Conjugate
    • Unconjugated
    • Related Disease
    • Smith-Kingsmore syndrome (SKS); Focal cortical dysplasia 2 (FCORD2)

    Product Property

    • Clonality
    • Monoclonal
    • Purity
    • >95% as determined by SDS-PAGE and SEC-HPLC analysis
    • Format
    • Liquid
    • Concentration
    • Please refer to the vial label for the specific concentration.
    • Buffer
    • PBS
    • Preservative
    • 0.05% sodium azide
    • Storage
    • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
    • Shipping
    • Ice packs

    Applications

    • Application Notes
    • Western Blot: 1:500 - 1:2000
      Immunohistochemistry-Paraffin: 1:200 - 1:1000
      Enzyme-linked Immunosorbent Assay: 1:10000

    Target

    • Alternative Names
    • MTOR; Rapamycin Associated Protein FRAP2; Rapamycin And FKBP12 Target 1; DJ576K7.1 (FK506 Binding Protein 12-Rapamycin Associated Protein 1); FRAP1; MTOR; FRAP; FKBP12-Rapamycin Complex-Associated Protein 1; Rapamycin Target Protein 1; FKBP-Rapamycin Asso
    • Long Name
    • Mechanistic Target Of Rapamycin Kinase
    • Cellular Localization
    • Cell membrane; Cytoplasm; Cytoplasmic vesicle; Endoplasmic reticulum; Golgi apparatus; Lysosome; Membrane; Microsome; Mitochondrion; Mitochondrion outer membrane; Nucleus
    • Post Translation Modifications
    • Acetylation; Isopeptide bond; Phosphoprotein; Ubl conjugation
    • Function
    • Kinase; Serine/threonine-protein kinase; Transferase

    Recommended Products

    Isotype Control

    Secondary Antibody

    Reconstitution Buffer

    Recommended Dilution Buffer

    Related Resources

    • Related Signaling Pathways
    • Related Diseases

    Product Notes

    This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

    • Increased sensitivity
    • Confirmed specificity
    • High repeatability
    • Excellent batch-to-batch consistency
    • Sustainable supply
    • Animal-free production

    See more details about Hi-Affi™ recombinant antibody benefits.

    Downloads

    Download resources about recombinant antibody development and antibody engineering to boost your research.

    See other products for "MTOR"

    Select a product category from the dropdown menu below to view related products.
    Please select product type
    Rabbit Monoclonal Antibody Products IgG Antibody Products IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
    CAT Product Name Application Type
    MOR-2318 Hi-Affi™ Recombinant Rabbit Anti-MTOR Monoclonal Antibody (DS2318AB) ICC/IF, IHC-P, WB IgG
    MOR-4652 Hi-Affi™ Recombinant Rabbit Anti-MTOR Monoclonal Antibody (TH165DS) WB, IF, ICC, FC IgG

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